Air monitoring of aldehydes by use of hydrazine reagents with a triazine backbone

Abstract

Two hydrazine reagents, 4- N, N-dimethylamino-6-(4'-methoxy-1'-naphthyl)-1,3,5-triazine-2-hydrazine (DMNTH) and N-methyl-4- N', N'-dimethylamino-6-(4'-methoxy-1'-naphthyl)-1,3,5-triazine-2-hydrazine (MDMNTH) have been synthesized and used for the determination of aldehydes in air samples. Test tubes with the reagents coated on silica gel were prepared and used for monitoring of carbonyls in air. After elution with acetonitrile the hydrazones formed were separated by reversed-phase liquid chromatography. Detection was performed by UV-visible and fluorescence spectroscopy. The results were validated by use of standard atmospheres of the carbonyls and of nitrogen dioxide and ozone, as potential interferents. In comparison with established hydrazine reagents, e.g. 2,4-dinitrophenylhydrazine (DNPH), the results from use of MDMNTH correlate well; lower recoveries were obtained by use of DMNTH. The limits of detection for the new reagents are superior to those for DNPH, because of the possibility of fluorescence detection.

Experimental choices for the determination of carbonyl compounds in air

“…Low limits of detection and high derivatization yields [13] ( Table 3). Electrochromatography offers the advantages of high separation efficiency to deal with liquid samples similar to HPLC [18].…”

“…Humidity can be a serious problem for carbonyl measurement by the HPLC method [13,37]. Ferrari et al [4] reported that saturated aliphatic (or aromatic aldehyde) hydrazones are not affected by water, whilst acetone hydrazone is strongly hydrolyzed in the presence of up to 10% water (in trapping acetonitrile solution in the impinger technique).…”

“…Aromatic hydrazines are the most popular derivatizing agents for carbonyl analysis, because they react to form the respective stable crystalline solid hydrazones in the presence of acidic catalysts [13]. In view of the above, the nitroaromatic hydrazines have been established as the most important derivatizing agents, while 4-nitrophenyl hydrazine (pNPH) became the traditional representative of this class [16].…”

The use of hydrazine-based derivatization reagents for improved sensitivity and detection of carbonyl containing compounds using MALDI-MSI

“…Test tubes coated with DMNTH have been used for the determination of aldehydes in air samples followed by chromatographic separation and detection by UV-Vis and fluorescence spectroscopy, the results were found to be comparable to the established method using the derivatization reagent DNPH [13]. The most recent application of DMNTH reported [9], is for the derivatization and analysis of a range of carbonyl containing compounds by laser desorption ionisation-mass spectrometry (LDI-MS).…”

Determination of triazines in hemodialysis saline solutions by adsorptive stripping voltammetry after extraction in acetonitrile

“…Although there is a number of methods employed to assay pesticides using different detectors as fluorimetric, [11][12][13][14][15][16][17] photometric [18][19][20] and electrochemical, [21][22][23] some problems still arise associated with the complexity of the matrices, particularly in the case of HS solutions where the ionic strength is so high that the common methods frequently fail. To overcome this difficulty, large dilution factors are often used but with prejudice to the sensitivity of the methods.…”