Airborne bacterial community and antibiotic resistome in the swine farming environment: Metagenomic insights into livestock relevance, pathogen hosts and public risks

Gao, Fangliang; He, Liang-Ying; Bai, Hong; He, Lu-Xi; Zhang, Min; Chen, Ziyin; Liu, You-Sheng; Ying, Guang‐Guo

doi:10.1016/j.envint.2023.107751

Cited by 27 publications

(12 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At the phylum level, ARG hosts consistently belong to Proteobacteria , Firmicutes , and Actinobacteria , regardless of the sampling site. Previous studies have reported that these phyla not only constitute the major bacterial taxa in various atmospheric environments (e.g., urban, rural, mountainous, and coastal areas) , but also regularly act as dominant ARG hosts in many other airborne ARG hotspots, such as hospitals, WWTPs, and livestock farms. ,, Such ecological niche preferences may be attributed to the intense UV resistance and low nutrient requirements of bacteria from these phyla. , At the species level, over 50% of ARG hosts were identified as pathogenic bacteria (TC:27/49; MC:32/60) with A. johnsonii predominating across different sampling sites. Especially, the culture-based study has demonstrated the presence of multidrug-resistant A. johnsonii in both FWTP-air environments (Figure S10).…”

Section: Discussionmentioning

confidence: 99%

“…Driven by the “One Health” perspective, the spread of antibiotic resistance genes (ARGs) between humans and various environmental reservoirs has gained widespread attention. , Compared with the hydrosphere and pedosphere, ARGs in the atmosphere are more closely and directly implicated in human health . In particular, airborne fine particulate matter (PM 2.5 )-related ARGs aggravate this health concern, as PM 2.5 can penetrate the body, reaching lung cavities and even entering the circulation. , Nonetheless, PM-associated resistome in atmospheric settings has only recently been explored, , with a particular focus on heavily antibiotic-polluted environments like livestock farms, hospitals, wastewater treatment plants (WWTP), landfills, and animal manure composting. − These studies imply that airborne ARGs play a critical role in disseminating environmental AMR to humans and that their exposure profiles are closely related to emission sources.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pathogenic Bacteria Are the Primary Determinants Shaping PM_2.5-Borne Resistomes in the Municipal Food Waste Treatment System

Zhang,

Wang,

et al. 2023

Environ. Sci. Technol.

View full text Add to dashboard Cite

Bioaerosol pollution poses a substantial threat to human health during municipal food waste (FW) recycling. However, bioaerosol-borne antibiotic-resistant genes (ARGs) have received little attention. Herein, 48 metagenomic data were applied to study the prevalence of PM2.5-borne ARGs in and around full-scale food waste treatment plants (FWTPs). Overall, FWTP PM2.5 (2.82 ± 1.47 copies/16S rRNA gene) harbored comparable total abundance of ARGs to that of municipal wastewater treatment plant PM2.5 (WWTP), but was significantly enriched with the multidrug type (e.g., AdeC/I/J; p < 0.05), especially the abundant multidrug ARGs could serve as effective indicators to define resistome profiles of FWTPs (Random Forest accuracy >92%). FWTP PM2.5 exhibited a decreasing enrichment of total ARGs along the FWTP–downwind–boundary gradient, eventually reaching levels comparable to urban PM2.5 (1.46 ± 0.21 copies/16S rRNA gene, N = 12). The combined analysis of source-tracking, metagenome-assembled genomes (MAGs), and culture-based testing provides strong evidence that Acinetobacter johnsonii-dominated pathogens contributed significantly to shaping and disseminating multidrug ARGs, while abiotic factors (i.e., SO4 2–) indirectly participated in these processes, which deserves more attention in developing strategies to mitigate airborne ARGs. In addition, the exposure level of FWTP PM2.5-borne resistant pathogens was about 5–11 times higher than those in urban PM2.5, and could be more severe than hospital PM2.5 in certain scenarios (<41.53%). This work highlights the importance of FWTP in disseminating airborne multidrug ARGs and the need for re-evaluating the air pollution induced by municipal FWTP in public health terms.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pathogenic Bacteria Are the Primary Determinants Shaping PM_2.5-Borne Resistomes in the Municipal Food Waste Treatment System

Zhang,

Wang,

et al. 2023

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…Then, the wastes were used for agricultural fertilization or transported to treatment plants for centralized treatment. To this end, the water systems, soil and air had been contaminated by farming activities [7,10,32,33].…”

Section: Study Area and Sample Collectionmentioning

confidence: 99%

Swine farm groundwater is a hidden hotspot for antibiotic-resistant pathogenic Acinetobacter

Gao

Chen

et al. 2023

ISME Communications

Self Cite

View full text Add to dashboard Cite

Acinetobacter is present in the livestock environment, but little is known about their antibiotic resistance and pathogenic species in the farm groundwater. Here we investigated antibiotic resistance of Acinetobacter in the swine farm groundwater (JZPG) and residential groundwater (JZG) of a swine farming village, in comparison to a nearby (3.5 km) non-farming village (WTG) using metagenomic and culture-based approaches. Results showed that the abundance of antibiotic resistome in some JZG and all JZPG (~3.4 copies/16S rRNA gene) was higher than that in WTG (~0.7 copies/16S rRNA gene), indicating the influence of farming activities on both groundwater types. Acinetobacter accounted for ~95.7% of the bacteria in JZG and JZPG, but only ~8.0% in WTG. They were potential hosts of ~95.6% of the resistome in farm affected groundwater, which includes 99 ARG subtypes against 23 antibiotic classes. These ARGs were associated with diverse intrinsic and acquired resistance mechanisms, and the predominant ARGs were tetracyclines and fluoroquinolones resistance genes. Metagenomic binning analysis elucidated that non-baumannii Acinetobacter including A. oleivorans, A. beijerinckii, A. seifertii, A. bereziniae and A. modestus might pose environmental risks because of multidrug resistance, pathogenicity and massive existence in the groundwater. Antibiotic susceptibility tests showed that the isolated strains were resistant to multiple antibiotics including sulfamethoxazole (resistance ratio: 96.2%), levofloxacin (42.5%), gatifloxacin (39.0%), ciprofloxacin (32.6%), tetracycline (32.0%), doxycycline (29.0%) and ampicillin (12.0%) as well as last-resort polymyxin B (31.7%), colistin (24.1%) and tigecycline (4.1%). The findings highlight potential prevalence of groundwater-borne antibiotic-resistant pathogenic Acinetobacter in the livestock environment.

show abstract

“…The use of other antibiotics like oxytetracycline, sulfamethoxazole, or enrofloxacin similarly induced rise of antibiotic resistance gene (ARG) levels or emergence of new antibiotic resistant bacteria (ARB) across a wide range of habitats 6 , 7 . These ARGs or ARB possess the potential to disseminate into the environment 8 – 10 , thereby heightening their risks in global public health sector 11 , 12 . Accordingly, the excessive use of antibiotics overshadows the sustainable development of the aquaculture industry, and particularly in the global context of “One Health,” novel antibacterial agents are urgently needed.…”

Section: Introductionmentioning

confidence: 99%

Bacteriophage EPP-1, a potential antibiotic alternative for controlling edwardsiellosis caused by Edwardsiella piscicida while mitigating drug-resistant gene dissemination

Han,

Huang,

Liu

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Edwardsiella piscicida causes significant economic losses to the aquaculture industry worldwide. Phage-based biocontrol methods are experiencing a renaissance because of the spread of drug-resistant genes and bacteria resulting from the heavy use of antibiotics. Here, we showed that the novel Edwardsiella phage EPP-1 could achieve comparable efficacy to florfenicol using a zebrafish model of Edwardsiella piscicida infection and could reduce the content of the floR resistance gene in zebrafish excreta. Specifically, phage EPP-1 inhibited bacterial growth in vitro and significantly improved the zebrafish survival rate in vivo (P = 0.0035), achieving an efficacy comparable to that of florfenicol (P = 0.2304). Notably, integrating the results of 16S rRNA sequencing, metagenomic sequencing, and qPCR, although the effects of phage EPP-1 converged with those of florfenicol in terms of the community composition and potential function of the zebrafish gut microbiota, it reduced the floR gene content in zebrafish excreta and aquaculture water. Overall, our study highlights the feasibility and safety of phage therapy for edwardsiellosis control, which has profound implications for the development of antibiotic alternatives to address the antibiotic crisis.

show abstract

Airborne bacterial community and antibiotic resistome in the swine farming environment: Metagenomic insights into livestock relevance, pathogen hosts and public risks

Cited by 27 publications

References 72 publications

Pathogenic Bacteria Are the Primary Determinants Shaping PM_2.5-Borne Resistomes in the Municipal Food Waste Treatment System

Pathogenic Bacteria Are the Primary Determinants Shaping PM_2.5-Borne Resistomes in the Municipal Food Waste Treatment System

Swine farm groundwater is a hidden hotspot for antibiotic-resistant pathogenic Acinetobacter

Bacteriophage EPP-1, a potential antibiotic alternative for controlling edwardsiellosis caused by Edwardsiella piscicida while mitigating drug-resistant gene dissemination

Contact Info

Product

Resources

About

Airborne bacterial community and antibiotic resistome in the swine farming environment: Metagenomic insights into livestock relevance, pathogen hosts and public risks

Cited by 27 publications

References 72 publications

Pathogenic Bacteria Are the Primary Determinants Shaping PM2.5-Borne Resistomes in the Municipal Food Waste Treatment System

Pathogenic Bacteria Are the Primary Determinants Shaping PM2.5-Borne Resistomes in the Municipal Food Waste Treatment System

Swine farm groundwater is a hidden hotspot for antibiotic-resistant pathogenic Acinetobacter

Bacteriophage EPP-1, a potential antibiotic alternative for controlling edwardsiellosis caused by Edwardsiella piscicida while mitigating drug-resistant gene dissemination

Contact Info

Product

Resources

About

Pathogenic Bacteria Are the Primary Determinants Shaping PM_2.5-Borne Resistomes in the Municipal Food Waste Treatment System

Pathogenic Bacteria Are the Primary Determinants Shaping PM_2.5-Borne Resistomes in the Municipal Food Waste Treatment System