Airway hyperreactivity follows anaphylactic microshock in anaesthetized guinea-pigs

Daffonchio, L.; Payne, A.N.; Lees, Ian W.; Whittle, Brendan J.R.

doi:10.1016/0014-2999(89)90835-2

Cited by 26 publications

(13 citation statements)

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“…The present study has confirmed that acute allergic bronchospasm in the guinea pig is not necessarily associat ed with airway hyperreactivity (10) and that minimal aller gic reactions are associated with expression of airway hyperreactivity (11). The inability of acute allergic bron chospasm to lead to expression of airway hyperreactivity cannot be attributed to suppression of airway responses by endogenous hormones (such as adrenaline or glucocor ticosteroids), since there was no impairment of responsiv ity to histamine in these animals; moreover, comparable acute airway obstruction following infusion of PAF was accompanied by unequivocal airway hyperreactivity.…”

Section: Discussionsupporting

confidence: 80%

“…It might be pre sumed that hyperreactivity to allergic mediators contrib utes to allergic bronchospasm; however, allergic hyper reactivity remains poorly defined in the guinea pig, even though this phenomenon is well documented for irritants (1)(2)(3), drugs (4-7) and inflammatory mediators (8,9). Thus, no increased responsivity to acetylcholine or histamine was detected in actively sensitized guinea pigs (10), yet hyperreactivity of the airways to inhaled hista mine was reported to follow anaphylactic microshock (11).…”

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confidence: 99%

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Allergic Bronchospasm and Airway Hyperreactivity in the Guinea Pig

Hoshiko

Morley

1993

Japanese Journal of Pharmacology

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ABSTRACT-In passively sensitized guinea pigs, slow infusion of an amount of ovalbumin insufficient to evoke airway obstruction induces hyperreactivity of the airways. A wide range of changed responsivity was observed for different test spasmogens, with leukotriene C4 > histamine > prostaglandin Fl, > bradykinin > leukotriene E4> serotonin > acetylcholine. Injection of ovalbumin as a bolus produced pronounced airway obstruction without hyperreactivity. Airway obstruction due to vascular engorgement (dextran infusion) or edema (histamine infusion) did not result in hyperreactivity. Infusion of PAF induced pronounced airway obstruction together with hyperreactivity, but with a rank order of histamine>leukotriene C4> serotonin > bradykinin > leukotriene E4 > acetylcholine. It can be concluded that allergic airway hyper reactivity in the guinea pig is spasmogen-selective and largely independent of airway obstruction. These observations question the presumption of non-selective hyperreactivity in allergic asthma and cast doubt upon the proposal that airway hyperreactivity is secondary to airway obstruction.

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Section: Discussionsupporting

confidence: 80%

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confidence: 99%

Allergic Bronchospasm and Airway Hyperreactivity in the Guinea Pig

Hoshiko

Morley

1993

Japanese Journal of Pharmacology

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“…Our results clearly show that in anaesthetized guinea-pigs, anaphylactic mediators released during an immediate type antigen-induced bronchoconstriction can indeed cause acute bronchial hyperresponsiveness as indicated by potentiation of the bronchoconstrictor effect of 5-HT. We have additionally shown in a preliminary report, that airway hyperreactivity induced in guinea-pigs by a challenge with antigen aerosol is not specific to 5-HT but extends also to acetylcholine (Daffonchio et al, 1987b). Furthermore, as this phenomena occurs after an 'immediate type' response, this shows that in this species a late 'asthmatic' response is not required for development of airway hyperreactivity.…”

Section: Discussionmentioning

confidence: 76%

“…This method has recently been characterized pharmacologically (Daffonchio et al, 1987a). Some of these results have been communciated to the British Pharmacological Society (Daffonchio et al, 1987b with an aqueous aerosol of OA, 10mg ml-' in saline, generated by a DeVilbiss 'Pulmosonic' Ultrasonic nebuliser (Payne & Nucci, 1987). As the resulting anaphylactic bronchoconstriction was to a large degree not spontaneously reversible, isoprenaline (Iso, 1pgkg-1min-1, i.v.)…”

Section: Introductionmentioning

confidence: 99%

Immediate anaphylactic bronchoconstriction induces airway hyperreactivity in anaesthetized guinea‐pigs

Daffonchio

Payne

Lees

et al. 1988

British J Pharmacology

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1 The possible acute occurrence of airway hyperreactivity after immediate-type bronchial anaphylaxis has been investigated in anaesthetized guinea-pigs actively sensitized to ovalbumin (OA). 2 Aerosol challenge (OA 10 mgml-1, 5s) provoked immediate bronchoconstriction which was substantially, although incompletely, reversed by isoprenaline (Iso) infusion (1 ,igkg1 min 1) for 10 min). 3 Bronchoconstrictor responses to 5-hydroxytryptamine (5-HT) were enhanced in challenged animals when compared to those in non-challenged animals that had also received Iso. This was seen as a leftward shift in the location of the dose-response curve for the bronchoconstrictor effect of 5-HT (dose-ratio 2.45, 95% confidence limits 1.77-3.38; P < 0.01). This phenomenon was associated with pulmonary infiltration of polymorphonuclear leukocytes, which was not modified by Iso treatment.4 Iso infusion alone caused a slight enhancement of airway reactivity seen as a small leftward shift of the dose-response curve for the bronchoconstrictor effect of 5-HT (dose-ratio 1.51, 95% confidence limits 1.07-2.13; P < 0.05). 5 These results support a causal relationship between acute pulmonary inflammation and airway hyperreactivity in an animal model of human allergic asthma.

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“…The guinea-pig is well suited for such studies since airway hyperreactivity and eosinophilia can readily be demonstrated in this species (Kallos & Kallos, 1984;Dunn et al, 1988;Hutson et al, 1988a;Aoki et al, 1988;Daffonchio et al, 1989). Exposure of sensitized guineapigs to aerosols containing a small dose of allergen induced an allergic reaction without anaphylactic bronchospasm.…”

Section: Discussionmentioning

confidence: 99%

Antigen challenge induces pulmonary airway eosinophil accumulation and airway hyperreactivity in sensitized guinea‐pigs: the effect of anti‐asthma drugs

Sanjar

Kristersson

et al. 1990

British J Pharmacology

188

114

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Guinea‐pigs were sensitized with 3 injections of ovalbumin (OA) (1 or 10 μg per animal) using Al(OH)3 and pertussis vaccine as adjuvants at two week intervals. Sensitized guinea‐pigs were challenged with an aerosol of OA (0.1%) over a one hour period and both airway reactivity and cellular content of bronchoalveolar lavage (BAL) fluid were assessed at intervals for up to 7 days. Guinea‐pigs sensitized with 1 μg of ovalbumin responded to an aerosol of OA with increased pulmonary airway eosinophilia, which was evident 1 day after challenge and was present for up to 7 days. Airway hyperreactivity was not detectable in these animals. Guinea‐pigs sensitized with 10 μg of ovalbumin responded to an aerosol of OA with increased pulmonary airway neutrophilia and eosinophilia and with increased airway reactivity which was maximal between 8 and 24 h after exposure to OA. Depletion of circulating platelets or neutrophils, by use of selective antisera, did not alter either the magnitude of eosinophilia or the intensity of airway reactivity in sensitized guinea‐pigs (10 μg) exposed to an aerosol of OA. Pretreatment of sensitized guinea‐pigs (10 μg) for 6 days with AH 21–132, aminophylline, dexamethasone or ketotifen inhibited pulmonary airway eosinophilia, but did not diminish airway hyperreactivity. Neither eosinophil accumulation nor development of airway hyperreactivity was influenced by treatment with mepyramine or salbutamol over a 6 day period before OA inhalation. Although eosinophilia may occur in association with increased airway reactivity in this animal model, there is no evidence of a causal relationship.

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Airway hyperreactivity follows anaphylactic microshock in anaesthetized guinea-pigs

Cited by 26 publications

References 26 publications

Allergic Bronchospasm and Airway Hyperreactivity in the Guinea Pig

Allergic Bronchospasm and Airway Hyperreactivity in the Guinea Pig

Immediate anaphylactic bronchoconstriction induces airway hyperreactivity in anaesthetized guinea‐pigs

Antigen challenge induces pulmonary airway eosinophil accumulation and airway hyperreactivity in sensitized guinea‐pigs: the effect of anti‐asthma drugs

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