This study aims to reveal the relationship between public service motivation and mobbing levels of public employees and the level of internalization of the mission. For this purpose, data were collected from 461 academics from different titles working in public universities operating in Türkiye. The obtained data were first subjected to Pearson correlation analysis and then hierarchical regression analysis. According to the correlation analysis findings, there is a statistically significant relationship between public service motivation and the internalization of the mission in the positive direction and between mobbing and the internalization of the mission in the adverse order. In the first model of the hierarchical regression analysis, it was observed that there was a positive significant relationship between the dimensions of public service motivation and the internalization of the mission. In the second model, mobbing dimensions were added to the analysis. According to the analysis results, the dimensions of attacks on self-expression and communication and attacks on professional status negatively predict the internalization of the mission. As a result, there is a statistically significant relationship between public service motivation, mobbing, and internalization of the mission. However, the public service motivations of academics who are mobbed for self-expression and communication, and professional status are negatively affected, and this may cause a decrease in the level of internalization of the mission.