AKD-2A, B, C and D, New Antibiotics from Streptomyces sp. OCU-42815. Taxonomy, Fermentation, Isolation, Structure Elucidation and Biological Activity.

Akeda, Yukihiro; Shibata, Kozo; Xu, Ping; Tanaka, Toshio; Taniguchi, Makoto

doi:10.7164/antibiotics.48.363

Cited by 16 publications

(5 citation statements)

References 7 publications

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“…Antimicrobial activities of nucleotide analogues were assayed by the serial broth dilution method which has been conventionally used in our studies on naturally occurring antibiotics (Akeda et al, 1995 ;Ueki et al, 1996). In this method, the MIC represents the minimum concentration of nucleotide analogue which can absolutely prevent the turbidity increase due to cell growth (Baltch et al, 1998 ;Kohner et al, 1997).…”

Section: Methodsmentioning

confidence: 99%

Long-chain alkyl ester of AMP acts as an antagonist of glucose-induced signal transduction that mediates activation of plasma membrane proton pump in Saccharomyces cerevisiae

et al. 2000

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One of the long-chain alkyl esters of AMP, adenosine 5'-hexadecylphosphate (AMPC16), exhibited a cytotoxic growth inhibitory effect on cells of various yeast strains. The growth inhibitory effect of AMPC16 on Saccharomyces cerevisiae cells was observed only in medium containing Mg 2M , which accelerated cellular uptake of the nucleotide analogue. In the presence of Mg 2M , AMPC16 completely inhibited glucose-induced extracellular acidification by the intact cells and also interfered with activation of the plasma membrane ATPase, but did not directly inhibit the ATPase activity itself. AMPC16 treatment prevented cells from increasing their intracellular sn-1,2-diacylglycerol (DAG) level in response to glucose, whereas the inhibition of proton extrusion by the cells could be largely reversed by the coaddition of a membrane-permeable DAG analogue. The DAG analogue, a physiological activator of protein kinase C (PKC), was not protective against the inhibition of glucose-induced proton extrusion by staurosporine, which is capable of directly interfering with the action of PKC. These results implied that AMPC16 caused a Mg 2M -dependent cytotoxic effect on Sac. cerevisiae cells by interfering with a phosphatidylinositol type of signal that mediates activation of the plasma membrane proton pump.

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Section: Methodsmentioning

confidence: 99%

Long-chain alkyl ester of AMP acts as an antagonist of glucose-induced signal transduction that mediates activation of plasma membrane proton pump in Saccharomyces cerevisiae

et al. 2000

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“…Unless stated otherwise, S. cerevisiae (Machida et al, 1997) was used throughout. Some other yeast strains, filamentous fungi and bacteria (see Results and Discussion) were also used for FOH antimicrobial activity assays by the serial broth dilution method as described previously (Akeda et al, 1995). Growth properties of S. cerevisiae X2180-1A cells were examined at 30 "C in YPD medium, which contained 1 YO yeast extract, 2 O/O polypeptone and 2 ' / O glucose.…”

Section: Methodsmentioning

confidence: 99%

Farnesol-induced growth inhibition in Saccharomyces cerevisiae by a cell cycle mechanism

et al. 1999

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The growth of budding yeast, Saccharomyces cerevisiae, was inhibited in medium containing 25 pM farnesol (FOH). The FOH-treated cells were still viable, and were characterized by a transition from budded to unbudded phase as well as a significant loss of intracellular diacylglycerol (DAG). FOH-induced growth inhibition could be effectively prevented by the coaddition of a membrane-permeable DAG analogue which can activate yeast protein kinase C (PKC). However, yeast cell growth was not initiated upon addition of the PKC activator when the cells had been pretreated with FOH for 20 min. The failure in cell growth recovery was believed to be due to a signalling-mediated cell cycle arrest in FOH-pretreated cells. Differential display analysis demonstrated that the expression of cell cycle genes encoding DNA ligase (CDCS) and histone acety I transf era se (HA T2) was strong I y repressed in FOH-treated ce I I s.Repression of the expression of these genes was effectively cancelled when cells were grown in medium supplemented with DAG. The authors propose an interference with a phosphatidylinositol-type signalling which is involved in cell cycle progression as a cause of FOH-induced growth inhibition in yeast cells.

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“…Aggreceride B was also detected in isolate MK65 with an accurate mass of 331.2843 dalton, which is a platelet aggregation inhibitor derived before from Streptomyces sp. ( 31 ). Also, stacopin P-1 and stacopin P-2 were detected in this study, which are proteinase inhibitors produced by Staphylococcus sp.…”

Section: Discussionmentioning

confidence: 99%

Exploring clinically isolated Staphylococcus sp. bacteriocins revealed the production of amonabactin, micrococcin, and α-circulocin

Kassem¹,

Saafan²,

Bayomy³

et al. 2021

IJM

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Background and Objectives: Bacteriocins are considered alternative non-conventional antimicrobials produced by certain bacteria with activity against closely related species. The present study focuses on screening, characterization, and partial purification of bacteriocins produced by Staphylococcus sp. isolated from different clinical sources such as pus and blood. Materials and Methods: A total of 100 Staphylococcus isolates were screened for bacteriocin production using spot on lawn assay and agar diffusion method against five indicator bacteria. Bacteriocins from five selected highly active isolates were subjected to proteinase-K enzyme, different pH, and heating at different temperatures, and investigated the stabilities of their antimicrobials. Two selected isolates, MK65 and MK88, were molecularly identified by 16S rRNA gene sequencing, explored for the presence of 18 bacteriocin genes, and liquid chromatography-high resolution electrospray ionization mass spectrometry (LC-HRESIMS) was used to identify their different metabolites. Results: Twenty isolates exhibited inhibitory effect against at least one indicator bacteria. Micrococcus luteus ATCC 4698 showed the highest sensitivity to such bacteriocins. Proteinase K, acidic pH, and heating at 100°C triggered marked activity inhibition. However, amylase enzyme, alkaline pH, and heating at 80°C caused trivial effects. Four out of eighteen bacteriocin genes were detected using PCR. Fermentation, partial purification, and LC-HRESIMS of total protein extracts of two selected isolates, MK65 and MK88, revealed the production of different antimicrobial peptides. Conclusion: To the best of our knowledge, this is the first study to report the production of micrococcin and α-circulocin from Staphylococcus aureus MK65 and the production of amonabactin from Staphylococcus epidermidis MK88.

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AKD-2A, B, C and D, New Antibiotics from Streptomyces sp. OCU-42815. Taxonomy, Fermentation, Isolation, Structure Elucidation and Biological Activity.

Cited by 16 publications

References 7 publications

Long-chain alkyl ester of AMP acts as an antagonist of glucose-induced signal transduction that mediates activation of plasma membrane proton pump in Saccharomyces cerevisiae

Long-chain alkyl ester of AMP acts as an antagonist of glucose-induced signal transduction that mediates activation of plasma membrane proton pump in Saccharomyces cerevisiae

Farnesol-induced growth inhibition in Saccharomyces cerevisiae by a cell cycle mechanism

Exploring clinically isolated Staphylococcus sp. bacteriocins revealed the production of amonabactin, micrococcin, and α-circulocin

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