Aktivitas Antibakteri Ekstrak Etanol Daun Gamal (Gliricidia sepium) pada Berbagai Konsentrasi terhadap Pertumbuhan Bakteri  Streptococcus mutans secara In-Vitro

Artaningsih, Ni Luh Budi; Habibah, Nur; Mastra, Nyoman

doi:10.26630/jk.v9i3.967

Cited by 7 publications

(15 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The mechanism of flavonoids in inhibiting nucleic acids is carried out through ring B on flavonoids which have an important role in the intercalation process or hydrogen bonding by accumulating nucleic acid bases that inhibit DNA and RNA synthesis which results in the destruction of lysosomes and bacterial microsomes. 16,17 This study is supported by a study by Pertiwi et al (2019) about the extract of the leaves of the kelakai can inhibit the growth of gram-positive bacteria, namely Streptococcus sanguinis. Grampositive bacteria have a cell wall structure consisting of less lipids, more peptidoglycan and contain polysaccharides (teichotic acid).…”

Section: Discussionsupporting

confidence: 65%

“…The first active substances that will work against Streptococcus mutans bacteria are alkaloids, tannins and saponins which will disrupt the outermost layer of bacteria, namely the peptidoglycan wall. 16 Peptidoglycan is a component of the cell wall of Streptococcus mutans bacteria so that the presence of this disorder will result in the cell wall layer being not fully formed and causing bacterial cell death. 17 The mechanism of action of tannins as antibacterial against Streptococcus mutans bacteria is by interfering with the synthesis of peptidoglycan compounds in bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…In Streptococcus mutans bacteria the molecule in the cytoplasm of the peptidoglycan cell wall serves to protect bacteria from high internal osmotic pressure, in the presence of tannin compounds, the formation of bacterial cell walls is inhibited or incomplete and in the presence of osmotic or physical pressure, bacterial cells will lyse. 16,18,19 Tannins also inhibit the enzyme reverse transcriptase and DNA topoisomerase so that bacterial cells cannot be formed. 20 The mechanism of action of saponins as antibacterial is by reducing the surface tension of the bacterial cell wall and increasing membrane permeability.…”

Section: Discussionmentioning

confidence: 99%

“…21,22 When the bacterial wall is not perfectly shaped and is damaged, the next secondary active substance, namely flavonoids, will easily enter into the cell, the cellular part of the bacteria and damage the bacterial nucleus. 16 The mechanism of action of flavonoids as antibacterial agents against Streptococcus mutans bacteria works by inhibiting the function of bacterial cell membranes, namely binding to bacterial cell membranes and forming complex compound bonds with soluble extracellular proteins so that the integrity of bacterial cell membranes is disrupted followed by the release of intracellular compounds. Disturbances in cell membrane permeability will result in impaired ATP synthesis, membrane transport and bacterial movement.…”

Section: Discussionmentioning

confidence: 99%

“…This is in accordance with the theory that the higher the concentration used, the more active compounds contained so that the antibacterial activity will be greater. 16 Chlorhexidine gluconate 0.2% was used as a positive control in this study. Chlorhexidine gluconate 0.2% is a broad-spectrum antibacterial agent that is strong against gram-positive and gram-negative bacteria.…”

Section: Discussionmentioning

confidence: 99%

See 4 more Smart Citations

ANTIBACTERIAL EFFECTIVENESS OF Stenochlaena palustris LEAVES EXTRACT AGAINST THE GROWTH OF Streptococcus mutans

Novita¹,

Firdaus²,

Taufiqurrahman³

2022

Dentino

View full text Add to dashboard Cite

Background: Dental caries is a common chronic infectious disease of dental hard tissue resulting from the interaction between tooth structure, the microbial biofilm formed on the tooth surface, dietary as well as salivary influences. Streptococcus mutans are considered to be the main bacteria that play role in the formation of cariogenic biofilms. Chlorhexidine gluconate 0,2% is a commonly used mouthwash and considered as the gold standard. The long-term use of chlorhexidine gluconate 0,2% may cause side effects so an alternative mouthwash with natural ingredients is needed. Kelakai leaves extract contains bioactive compounds which has antimicrobial effects such as flavonoids, alkaloids, tannins and saponins which can be used as an alternative mouthwash for chlorhexidine gluconate 0.2%. Objective: The purpose of this study was to observe the antibacterial effectiveness of kelakai leaves extract with concentrations of 3.125%, 6.25%, 12.5%, 25%, 50%, 75% and 100% with chlorhexidine gluconate 0.2% on growth of Streptococcus mutans bacteria. Methods: This study conducted through true experimental laboratories with a post-test only research design with control group design. This study used 9 treatments with 5 repetitions so that there were a total of 45 samples. The treatment group was kelakai leaves extract with concentrations of 3.125%, 6.25%, 12.5%, 25%, 50%, 75% and 100% with two control groups namely chlorhexidine gluconate 0.2% and distilled water against Streptococcus mutans. Results: The MIC of kelakai leaves extract was set at a concentration of 12.5% and the MBC at a concentration of 50%. Conclusion: Kelakai leaves extract concentrations 50%, 75% and 100% has antibacterial effectiveness which equivalent to chlorhexidine gluconate 0,2% against Streptococcus mutans bacteria.Keywords: Antibacterial, Stenochlaena palustris leaves extract, Streptococcus mutans

show abstract

Section: Discussionsupporting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

ANTIBACTERIAL EFFECTIVENESS OF Stenochlaena palustris LEAVES EXTRACT AGAINST THE GROWTH OF Streptococcus mutans

Novita¹,

Firdaus²,

Taufiqurrahman³

2022

Dentino

View full text Add to dashboard Cite

show abstract

The Effect of The Gamal Leaf Ethanol Extract on The Growth of Escherichia coli Isolated from Semi Natural Sea Turtle Nest in Boom Beach, Banyuwangi

Hutahaean¹,

Meles²,

Praja³

et al. 2022

J. Med. Vet.

View full text Add to dashboard Cite

The aim of this study was to identify the antibacterial activity potention of gamal leaf (Gliricidia sepium) ethanol extract against Escherichia coli growth which isolated from Olive Ridley Sea Turtle (Lepidochelys olivacea) semi-natural nest in Boom beach, Banyuwangi. The antibacterial test used disk diffusion method. Oxytetracycline 30 µg (Oxoid CT0041B) was used as the Positive Control (K+). CMC Na 0,5% was used as the Negative Control (K-). The treatment (P1, P2, P3, P4) used various concentrations of gamal leaves ethanol extract, which is 40%, 50%, 60%, and 70%. The study assigned six treatments and four replication. The obtained data were analyzed by ANOVA, then continued with the DUNCAN test if the results showed significant differences (p<0,05). The inhibition result of positive control (K+) was 24,70c ± 7.04; and the negative control was 0,00a ± 0,00. All extract concentration result different inhibition, concentration 1 (P1) was 10,41 b ± 3,10; concentration 2 (P2) 8,14 b ± 0,45; concentration 3 (P3) 8,08 b ± 0,47; and concentration 4 (P4) 8,01b ± 0,29. These results indicated that the gamal leaves ethanol extract has antibacterial activity to inhibit the growth of E. coli isolated from Olive Ridley semi-natural nest in Boom beach, Banyuwangi.

show abstract

Efektivitas Antibakteri Ekstrak Biji Alpukat (Persea americana Mill.) terhadap Pertumbuhan Bakteri Streptococcus mutans

Sholekhah,

Hidayati,

Kustantiningtyastuti

2022

Andalas Dent. J.

View full text Add to dashboard Cite

Background : Streptococcus mutans bacteria are the main microorganisms that play an important role in the etiology of caries. Prevention of dental caries can be done by reducing the formation of plaque on the surface of the teeth, one of which is through the use of antibacterial compounds contained in avocado seed extract. Purpose : This study is to determine the antibacterial effectiveness of avocado seed extract in inhibiting the growth of Streptococcus mutans bacteria. Methods : This research method is an experimental laboratory with a post-test control group design. The avocado seed extract is made with a maceration technique with the result of a concentration of avocado seed extract of 100% then diluted using aquadest so that avocado seed extract concentrations of 10%, 15%, 20%, and 25% are obtained. The inhibitory test was done by Kirby-Bauer disk diffusion on Mueller Hinton Agar. The inhibition zone formed around the disc is measured using a caliper. The data analysis used was the One Way Anova test and continued with the Post Hoc LSD test. Results : The results showed that avocado seed extract at all concentrations can inhibit the growth of Streptococcus mutans bacteria. The largest inhibitory zone diameter at a concentration of 25% with a diameter of 12.25 mm and the diameter of the smallest inhibitory zone at a concentration of 10% at 10.48mm. the results of statistical analysis showed that there were significant differences between the inhibition zones of each avocado seed extract concentration (p<0.05).Conclusions : there is an effect of avocado seed extract (Persea americana Mill.) in inhibiting the growth of Streptococcus mutans bacteria with extract concentrations of 10%, 15%, 20%, and 25%.

show abstract

Aktivitas Antibakteri Ekstrak Etanol Daun Gamal (Gliricidia sepium) pada Berbagai Konsentrasi terhadap Pertumbuhan Bakteri Streptococcus mutans secara In-Vitro

Cited by 7 publications

References 4 publications

ANTIBACTERIAL EFFECTIVENESS OF Stenochlaena palustris LEAVES EXTRACT AGAINST THE GROWTH OF Streptococcus mutans

ANTIBACTERIAL EFFECTIVENESS OF Stenochlaena palustris LEAVES EXTRACT AGAINST THE GROWTH OF Streptococcus mutans

The Effect of The Gamal Leaf Ethanol Extract on The Growth of Escherichia coli Isolated from Semi Natural Sea Turtle Nest in Boom Beach, Banyuwangi

Efektivitas Antibakteri Ekstrak Biji Alpukat (Persea americana Mill.) terhadap Pertumbuhan Bakteri Streptococcus mutans

Contact Info

Product

Resources

About