AKTIVITAS ANTIOKSIDAN DAN PENGHAMBATAN Α-Glukosidase OLEH EKSTRAK ETANOL BAKTERI ASAM LAKTAT INDIGENUS

Abstract

Lactic acid b acteria (LAB) are one of I ndonesia's b iodiversity which can b e b eneficial for food and health purposes. Some of LAB are potential prob iotics with specific functional properties, such as antidiab etes. This study evaluated the effect of ethanol extracts of twelve indigenous LAB in inhib iting αglucosidase enzyme and their antioxidant activities. Assay for the α-glucosidase inhib ition was performed on LAB ethanol extract using spectrophotometric method at λ=410 nm, while the antioxidant activity was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH) method at λ=517 nm. The results showed that αglucosidase inhib ition was significantly different b etween the isolates (P<0.01). Lactob acillus fermentum S21209 had the highest α-glucosidase inhib ition activity, which was significantly different from Lactob acillus plantarum MB427, Lactob acillus plantarum Pi28a, Lactob acillus delb rueckii W24802 and Lactob acillus plantarum 2 W22409. Evaluation of the antioxidant activity also showed significant difference b etween the isolates (P<0.01). Lactob acillus plantarum BSL had the highest antioxidant activity (92.81±1.36%), which was not significantly different from vitamin C as a control. This preliminary study reported that twelve indigenous LAB could b e used as potential antidiab etic prob iotics, although the responsib le compounds are not known.

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