AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL Gracilaria sp. DAN Ulva sp. DARI PANTAI SAYANG HEULANG

Widowaty, Windy; Setiawan, Yopi; Perdana, Wildan Wibawa

doi:10.35194/agsci.v10i2.1163

Cited by 3 publications

(2 citation statements)

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“…Extract of Gracilaria sp. from Pamekasan waters reached an IC50 value of 308.19 ppm [10], from Pok Tunggal Beach waters reached an IC50 value of 188.53 ppm, from Ngandong waters reached an IC50 value of 168.76 ppm [9], and from Sayang Heulang waters reached an IC50 value of 221.76 ppm [12]. No related information has been obtained in Bintan Waters, even though this location has eight macroalgae species from 3 Divisions [13].…”

Section: Introductionmentioning

confidence: 95%

Bioactive Compounds and Antioxidant Activity of Tropical Red Algae Gracilaria sp. from Bintan Island, Indonesia

Hidayati

Karlina

Ningsih

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Macroalgae that grow in the tropics are continuously exposed to ultraviolet radiation and sunlight. Macroalgae have self-defense to avoid damage caused by ultraviolet radiation by carrying out secondary metabolism and producing several bioactive compounds. The bioactive compounds produced have the potential to be utilized to inhibit free radicals that can cause degenerative diseases in humans. This research aims to identify bioactive compounds and determine the antioxidant activity, total phenolic compound, total flavonoid compound, and pigment content of red algae Gracilaria sp. from Bintan island. This research has the novelty of the sampling location to be later developed into a sustainable resource in the pharmaceutical field. Gracilaria sp. found in Bintan island was taken, dried in indirect sunlight, and macerated using universal solvent methanol. Phytochemical testing was conducted to determine the presence of flavonoids, steroids, triterpenoids, saponins, and tannins. Antioxidant activity was determined using DPPH as a free radical at the maximum wavelength of DPPH (515 nm) with an incubation time of 55 minutes. Total phenolic and flavonoid compounds were determined using standard solutions of gallic acid and quercetin, respectively. The results showed that the methanol extract of Gracilaria sp. contains flavonoid and tannin compounds, has a chlorophyll-a content of 0.43 mg/g, a carotenoid content of 0.91 µmol/g, the total phenolic compound of 4.67 mg/GAE g, total flavonoid compound of 2.26 mg/QE g and has antioxidant activity with IC50 value of 982.25 ppm. Utilization of Gracilaria sp extract from Bintan waters as a pharmaceutical ingredient is expected to increase prospects and sustainable use in the pharmaceutical and health fields.

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Section: Introductionmentioning

confidence: 95%

Bioactive Compounds and Antioxidant Activity of Tropical Red Algae Gracilaria sp. from Bintan Island, Indonesia

Hidayati

Karlina

Ningsih

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…Beberapa penelitian tentang antioksidan ulvan menunjukkan nilai yang bervariasi. Penelitian Widowaty et al (2020) terhadap hasil ekstaksi Ulva sp. dengan campuran metanol, kloroform, dan buffer fosfat menghasilkan IC 50 sebesar 404,73 µg/ mL.…”

Section: Aktivitas Antioksidanunclassified

Potensi ulvan dari Ulva lactuca sebagai sumber antioksidan

Jacoeb,

Abdullah,

Hakimah

2024

JPHPI

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Dinding sel Ulva lactuca menghasilkan ulvan yang merupakan hidrokoloid bersulfat dan berpotensi sebagai antioksidan. Penelitian ini bertujuan untuk menentukan pengaruh lama pemanasan dalam autoklaf (15 dan 20 menit; 121℃) terhadap rendemen, kadar gula dan sulfat, serta aktivitas antioksidan ulvan. Rendemen ulvan, kandungan gula dan sulfat diukur masing-masing dengan metode gravimetri dan spektrofotometri. Ekstrak kering oven diuji gugus fungsi dan antioksidannya menggunakan FTIR dan metode DPPH. Ulvan hanya bisa dihasilkan melalui ekstraksi pada suhu 121℃ waktu 15 menit. Ekstrak kering ulvan memberikan rendemen 1,98%, kandungan gula total 46,06±0,16% dan kadar sulfat 21,53±1,16%. Spektrum FTIR menunjukkan adanya bilangan gelombang 850 dan 790 cm-1 yang merupakan ciri ulvan. Uji DPPH menghasilkan IC50 sebesar 469 ppm, yang tergolong sebagai antioksidan lemah.

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ANTIOXIDANT AND TOXICITY ACTIVITIES of Gracilaria gracilis METHANOL EXTRACT BASED ON DIFFERENT EXTRACTION METHODS

Panjaitan

Yuliana

2022

JKR

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Gracilaria gracilis (red macroalgae) was collected from Sayang Heulang Beach, Garut, West Java. It has a number of secondary metabolites that potentially serve as a source of natural antioxidants. The objective of this study was to compare the optimal extraction method in particular time variation for producing the highest percent of yield, antioxidant activity, and toxicity of G. gracilis methanol extract. This study was conducted experimentally, including macroalgae sampling and preparation, phytochemical screening, extraction of G. gracilis by maceration and UAE methods, antioxidant activity testing based on the DPPH test (2.2 diphenyl-1-picrylhydrazyl) and toxicity testing using the BSLT (Brine Shrimp Lethality) method. The results indicated that the methanol extract of G. gracilis contains flavonoids and steroids, with yield percentages of 12.93% for maceration and 12.1% for UAE. The antioxidant activity (IC50) of maceration was 86.46 ppm, whereas the UAE was 59.01 ppm. Then, the toxicity test (LC50) for maceration was 28.35 ppm whereas the UAE was 27.76 ppm. Macerated methanol extract and UAE G. gracilis have the potential to be powerful antioxidants based on their IC50 values. Then, macerated methanol extract and UAE are included in the highly toxic category.

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AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL Gracilaria sp. DAN Ulva sp. DARI PANTAI SAYANG HEULANG

Cited by 3 publications

References 0 publications

Bioactive Compounds and Antioxidant Activity of Tropical Red Algae Gracilaria sp. from Bintan Island, Indonesia

Bioactive Compounds and Antioxidant Activity of Tropical Red Algae Gracilaria sp. from Bintan Island, Indonesia

Potensi ulvan dari Ulva lactuca sebagai sumber antioksidan

ANTIOXIDANT AND TOXICITY ACTIVITIES of Gracilaria gracilis METHANOL EXTRACT BASED ON DIFFERENT EXTRACTION METHODS

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