Aktivitas Enzimatis Isolat Bakteri Asam Laktat dari Saluran Pencernaan Kepiting Bakau (Scylla spp.) Sebagai Kandidat Probiotik   &lt;br&gt;&lt;i&gt;[Activity Enzymatic of Isolate Lactic Acid Bacteria from the Digestive Tract of Mud Crab (Scylla spp.) as a Candidate Probiotics]&lt;i&gt;

Tjahjaningsih, Wahju; Masithah, Endang Dewi; Pramono, Heru; Suciati, Pipin

doi:10.20473/jipk.v8i2.11182

Cited by 13 publications

(12 citation statements)

References 4 publications

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“…The reshuffle of starch structure occurs due to the activity of amylase which is marked by a clear zone around the amylolytic bacteria. The media that does not show a clear zone and is blue when after dripping lugol indicates that the bacteria do not produce amylase enzyme [11]. The bacteria isolates that did not show a clear zone around is isolate A4 (Fig.…”

Section: Amylolytic Testmentioning

confidence: 99%

Pesticide Degrading Ability of Indigenous-Bacteria from Contaminated Cropland in Kersana, Brebes Regency, Indonesia

Rohayati¹,

Yuana²,

Yuningsih³

et al. 2023

Advances in Biological Sciences Research

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Kersana is one of the central shallot-producing districts in Brebes Regency. Synthetic pesticides are widely and usually used in this cropland, and it has caused pollution of soil and water. Bioremediation can be one of the solutions to overcome this problem by using indigenous-bacteria from contaminated soil and water as biological agents. This research was purposed to isolate and identify pesticide degrading bacteria from shallot fields in Kersana, Brebes regency. Twenty-three (23) isolates were obtained in the isolation process. Those isolates were tested for biochemical characterizations which are potassium hydroxide test, proteolytic test, amylolytic test, and catalase test and potential test which are hypersensitive reaction test and pesticide degradation ability test. Based on the biochemical test and pesticide degradation ability test, all isolates have an ability to live under a pesticide contaminated environment and thirteen (13) isolates were suspected to be safe for application in the field. These results suggest that indigenous bacteria can help reduce pollution due to the use of pesticides in an environmentally friendly way.

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Section: Amylolytic Testmentioning

confidence: 99%

Pesticide Degrading Ability of Indigenous-Bacteria from Contaminated Cropland in Kersana, Brebes Regency, Indonesia

Rohayati¹,

Yuana²,

Yuningsih³

et al. 2023

Advances in Biological Sciences Research

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“…In addition, carbohydrate retention, fat retention, and protein retention will also increase due to the absorption of feed nutrients [26]. Microbes contained in probiotics are usually gram-positive bacteria such as the genus Lactobacillus and Bifidobacterium [27].…”

Section: Treatmentmentioning

confidence: 99%

Probiotics-herbs of rhizome origin as feed additives and utilization in fish

Haetami¹,

Abun²,

Mulyani³

et al. 2023

Int. J. Front. Chem. Pharm. Res.

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Fish growth and survival depend on feed. The main factor in determining the quality of feed depends on its nutritional content. Physically and biologically extracted herbal ingredients are the characteristics of herbal probiotics. Herbal probiotics are made by adding microorganisms, enzymes, acidity regulators, minerals, vitamins, and other ingredients depending on the intended use and how to use them. This paper provides an explanation by way of a review method regarding herbal probiotics by adding microorganisms through a herbal fermentation process which and can then be used as a feed additive that has benefits for growth and increases the resistance or immunity of fish.

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“…Isolat BAL tersebut memiliki sifat morfologi dengan bentuk koloni bulat, berwarna putih atau putih susu, permukaan cembung, tepian rata. Sifat BAL yaitu katalase negatif, non motil dan memiliki produksi gas yang dibedakan menjadi homofermentatif atau heterofermentatif, serta pewarnaan Gram berwarna ungu (positif) dengan bentuk batang atau bulat (Suciati et al 2016). non MSG (absorbansi 1,488 pada jam ke-48) dan pada medium dengan penambahan MSG 5% (absorbansi 1,631 pada jam ke-42).…”

Section: Isolat Bal Dan Karakteristiknyaunclassified

Isolasi Dan Karakterisasi Bakteri Asam Laktat Dari Pangan Fermentasi Cincalok Sebagai Penghasil Gamma-Aminobutyric Acid

Pribadhi¹,

Kusdiyantini²,

Ferniah³

2021

J Bioteknol Biosains Indones

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Isolation and Characterization of Lactic Acid Bacteria from Fermented Food Cincalok as Producer of Gamma-Aminobutyric Acid Cincalok is a fermented food originating from West Kalimantan. This study aimed to obtain lactic acid bacterial isolates (LAB) capable of producing gamma-aminobutyric acid (GABA), to characterize the LAB isolates obtained, and to obtain GABA by the Thin Layer Chromatography (TLC) method. Bacterial growth and GABA production was carried out by adding 5% MSG and without MSG, and measured spectrophotometrically. In this study, 4 LAB bacterial isolates were obtained which were coded CIN-1, CIN-2, CIN-3, and CIN-4. GABA identification of all the LAB isolates using TLC Silica Gel 60 F254 with butanol: acetic acid: distilled water (5: 3: 2) as eluent yielded Rf 0.61 and Rf MSG 0.38. The highest growth was achieved by isolate CIN-3 with an absorbance of 1.488 (at 48 hour) in non-MSG medium, while the addition of 5% MSG resulted in an absorbance of 1.631 (at 42 hour). GABA production was achieved by isolate CIN-3 with 5% MSG treatment with a concentration of 201.472 mM and without MSG with a concentration of 171.195 mM. Cincalok merupakan pangan fermentasi yang berasal dari Kalimantan Barat. Penelitian ini bertujuan untuk mendapatkan isolat bakteri asam laktat (BAL) yang mampu menghasilkan gamma-aminobutyric acid (GABA), melakukan karakterisasi isolat BAL yang diperoleh dan dapat diperoleh GABA dengan metode Kromatografi Lapis Tipis (KLT). Penumbuhan bakteri dan produksi GABA dilakukan dengan penambahan MSG 5% dan tanpa MSG, dan diukur menggunakan spektrofotometer. Dalam penelitian ini diperoleh 4 isolat bakteri BAL yang diberi kode CIN-1, CIN-2, CIN-3, dan CIN-4. Identifikasi GABA dari semua isolat BAL tersebut menggunakan KLT Silica Gel 60 F254 dengan eluen butanol: asam asetat: aquades (5: 3: 2), menghasilkan Rf 0,61 dan Rf MSG 0,38. Pertumbuhan tertinggi terjadi pada isolat CIN-3 non MSG dengan absorbansi 1,488 (jam ke-48), sedangkan dengan penambahan MSG 5% menghasilkan absorbansi 1,631 (jam ke-42). Produksi GABA dicapai isolat CIN-3 dengan perlakuan MSG 5% dengan konsentrasi 201.472 mM dan tanpa MSG dengan konsentrasi 171,195 mM.

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Aktivitas Enzimatis Isolat Bakteri Asam Laktat dari Saluran Pencernaan Kepiting Bakau (Scylla spp.) Sebagai Kandidat Probiotik <br><i>[Activity Enzymatic of Isolate Lactic Acid Bacteria from the Digestive Tract of Mud Crab (Scylla spp.) as a Candidate Probiotics]<i>

Cited by 13 publications

References 4 publications

Pesticide Degrading Ability of Indigenous-Bacteria from Contaminated Cropland in Kersana, Brebes Regency, Indonesia

Pesticide Degrading Ability of Indigenous-Bacteria from Contaminated Cropland in Kersana, Brebes Regency, Indonesia

Probiotics-herbs of rhizome origin as feed additives and utilization in fish

Isolasi Dan Karakterisasi Bakteri Asam Laktat Dari Pangan Fermentasi Cincalok Sebagai Penghasil Gamma-Aminobutyric Acid

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