This study aimed to understand the effects of various energy-protein ratios of feed on nilem carp fish (Osteochilus hasselti) growth. This study was conducted from September to October 2017 at the Laboratory of Building 4, Faculty of Fisheries and Marine Sciences, Universitas Padjajaran. The average size of the test fish was ± 7 cm with an average weight of 3 grams. This was a Completely Randomized Design (CRD) experimental study consisting of 5 treatments and 3 repetition. The treatments given were 5 feed formulas based on different feed protein energy ratios, i.e. treatment A (31% protein, ratio 10), treatment B (31% protein, ratio 8), treatment C (28% protein, ratio 10), D (28% protein, ratio 8), and E (30% protein commercial feed/control). Feed, 5% of the test fish total weight, was provided 3 times a day. Parameters observed were daily growth rate, feed conversion, and survival. Data gained were analyzed using F-test, followed by Duncan Test if differences were found between treatments. Results showed that growth rate and feed conversion of all treatments were not different compared to control. The highest result was found in treatment A (31% protein, ratio 10) with a daily growth rate of 3.99% and feed conversion of 1.95%.

Abstract-

BLS from encapsulated of extract of shrimp waste bioconversion on feed supplement quality and its implication of metabolizable energy and digestibility at Indonesian local chicken,

Feed quality shown from level of digestibility can affect fish growth.Some of omnivorous fish have complete digestive organs as a place to live abiotic and biotic ecosystems in the form of living microflora. Growth performance can be improved through the addition of exogenous microflora as feed supplements to help produce simpler components of food substances (amino acids, fatty acids, simple sugars, organic vitamins and minerals). The microflora tested consisted of bacteria Bacillus sp. and fungi (Aspergillus niger and Saccharomycescereviseae) with optimization of its prebiotic bioprocess conditions (bioprocess temperature, inoculum dose, and bioprocess time). Furthermore, to see the quality and value of benefits of feed supplement products, measurements were taken of their digestibility.The experiment was carried out experimentally in a laboratory in two stages. The first stage, using a nested design (3×3) which was repeated three times. The second stage used a completely randomized design, consisting of six ration treatments and repeated four times. The variables observed in the first stage: nutrient content (crude protein, crude fibre, extract ether, calcium and phosphorus) of prebiotics BAS ; second stage: digestibility of dry matter and crude protein.The data were subjected to analysis of variance, and the differences between treatments were tested by Duncan's multiple range test.Conclussion: The following results were obtained the best bioprocess conditions for making Prebiotics BAS from Bacillus sp. was a dose of 2% with temperature of 45 o C, and fermentation time 2 days, while Aspergillus niger 2% at a temperature of 35 o Calong 2 days, and Saccharomyces cereviseae 2% with a temperature of 35 o C, and fermentation time 2 days. The use of a mixture of three types of microbial each a much 1.5% in the ration, resulting in the best digestibility value in fish. The dry matter and crude protein digestibility valueof Prebiotics BAS were respectively 76.07%, and 75.28%.

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