2019
DOI: 10.14710/bioma.21.2.91-99
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Aktivitas Inhibitor α-Amilase Ekstrak Etanol Tanaman Brotowali (Tinospora crispa L.)

Abstract: Brotowali plant (Tinospora crispa L.) is a traditional Indonesian medicinal plant which has many benefits including for diabetes drugs. Diabetes mellitus (DM) is a metabolic abnormality caused by an increase in blood sugar levels. The purpose of this study was to determine the activity of α-amylase inhibitors of Brotowali (T. crispa L.) ethanol extract of plants. Extraction is done by maceration followed by evaporation. The extract obtained was tested for α-amylase inhibitor activity. The α-amylase inhibition … Show more

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Cited by 4 publications
(5 citation statements)
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“…About 1 mL suspension was added to 150 mL PDB media, incubated at room temperature, and shaken at 120 rpm for 7 days. The cultures were centrifuged at 4000 rpm for 15 min and the inhibitory activity of the supernatant against alpha-amylase was measured at different substrate concentrations of 0.5%, 1%, 1.5%, 2.0%, and 2.5% (Pujiyanto et al, 2019).…”
Section: Substrate-based Optimization Assaymentioning
confidence: 99%
“…About 1 mL suspension was added to 150 mL PDB media, incubated at room temperature, and shaken at 120 rpm for 7 days. The cultures were centrifuged at 4000 rpm for 15 min and the inhibitory activity of the supernatant against alpha-amylase was measured at different substrate concentrations of 0.5%, 1%, 1.5%, 2.0%, and 2.5% (Pujiyanto et al, 2019).…”
Section: Substrate-based Optimization Assaymentioning
confidence: 99%
“…Aktivitas dari enzim α-amilase dan glukoamilase yang telah dihambat oleh ekstrak sampel tidak dapat bereaksi dengan substrat pati, sehingga intensitas warna yang dihasilkan akan semakin berkurang. Berdasarkan dari nilai absorbansi yang telah diperoleh, nilai persentase (%) penghambatan terhadap aktivitas enzim α-amilase dan glukoamilase dari ekstrak etanol daun kirinyuh dapat ditentukan yang ditunjukkan pada Tabel 1. semakin besar nilai hambatan [12]. Hasil dari nilai persentase penghambatan terhadap aktivitas enzim α-amilase dan glukoamilase dari variasi konsentrasi ekstrak etanol daun kirinyuh digunakan untuk membuat persamaan regresi linier untuk menghitung nilai IC50.…”
Section: Pengujian Aktivitas Penghambatan Enzim α-Amilase Dan Glukoam...unclassified
“…The concentration of ethyl acetate extract affected the inhibitory activity of the α-amylase enzyme, the highest increase in inhibitory activity was 84.63% at a concentration of 10% and the lowest inhibitory activity was 71.12% at a concentration of 2%, which means that each addition of extract concentration increased the activity of α-amylase inhibitors. Following the research of Pujiyanto et al (2019), the increase in the dilution concentration of the extract was directly proportional to the activity of the αamylase inhibitor, which means that the greater the concentration of the extract, the higher the percentage of inhibitor. The value of the scattered inhibitory activity was 95.06% at the extract concentration of 1000 g/mL, while the lowest αamylase inhibitor activity was -31.32% at the extract concentration of 62.5 g/mL.…”
Section: An α-Amylase Extract Ethyl Acetate Inhibitor Activity Testmentioning
confidence: 99%