The research about analysis of cadmium (Cd) and lead (Pb) metal content on sediment inner part of  Ambon Bay has been done. Sampling point located at Dermaga Ferry Poka, PLTD Poka, Kate-kate, Waiheru, Lateri III, Passo  dan PLTD Galala. The results of analyzed of sediment particle size inside the Ambon Bay showed 3 components were sand, gravel, and sludge, but the sediments was dominated by sludge sand. Analysis of Cd content showed that at 7 stations in Ambon Bay detected Cd metal with the highest concentration at 4th station of Waiheru village was 0,6640 mg/Kg and the lowest concentration at 3th station of Kate-kate village was 0,0980 mg/Kg. Meanwhile, the highest Pb content was 20,075 mg/kg at 4th station and the lowest was 5,755 mg/kg at 2nd station of PLN Poka. The content of elements composition of sediment are SiO2, K2O, P2O5, Fe2, BaO, NiO and Ga2O3 but Cd and Pb metal was not detected.

Research has been carried out to test the inhibitory activity of α-amylase and glucoamylase enzymes in the ethanolic extract of kirinyuh (Chromolaena odorata L.) leaves. The use of kirinyuh leaf ethanol extract as a diabetes drug in vivo has been carried out, but its mechanism of action have never been researched before, so further research is needed on the specific mechanism of ethanolic extract of kirinyuh leaves in reducing blood sugar levels, especially through inhibition of α-amylase and glucoamylase enzymes in vitro. The purpose of this study was to test the antidiabetic activity of the ethanol extract of kirinyuh leaves in vitro using α-amylase and glucoamylase inhibition methods. This research uses DNS spectrophotometric method. The results showed that kirinyuh leaf ethanol extract proved to have inhibitory activity against α-amylase and glucoamylase enzymes with IC50 values of 3730.15 ± 28.91 ppm or 3730.15 ± 28.91 µg/mL and 2510.78. ± 383.37 ppm or 2510.78 ± 383.37 µg/mL. From the results, it can be concluded that the ethanolic extract of kirinyuh leaves has inhibitory activity against α-amylase and glucoamylase enzymes.   Telah dilakukan penelitian uji aktivitas penghambatan enzim α-amilase dan glukoamilase ekstrak etanol daun kirinyuh (Chromolaena odorata L.). Penggunaan ekstrak etanol daun kirinyuh sebagai obat diabetes secara in vivo telah dilakukan,tetapi belum diketahui mekanisme kerjanya, sehingga perlu dilakukan penelitian lebih lanjut mengenai mekanisme spesifik ekstrak etanol daun kirinyuh pada penurunana kadar gula darah terutama melalui aktivitas penghambatan terhadap enzim α-amilase dan glukoamilase secara in-vitro. Tujuan penelitian ini untuk untuk menguji aktivitas antidiabetes ekstrak etanol daun kirinyuh secara in vitro menggunakan metode penghambatan enzim α-amilase dan glukoamilase. Penelitian ini menggunakan metode DNS secara spektrofotometri. Hasil penelitian menunjukkan ekstral etanol daun kirinyuh terbukti memiliki aktivitas penghambatan terhadap enzim α-amilase dan glukoamilase dengan nilai IC50 masing-masing, yaitu sebesar 3730,15 ± 28,91 ppm atau 3730,15 ± 28,91 μg/mL dan 2510,78 ± 383,37 ppm atau 2510,78 ± 383,37 μg/mL. Dari hasil dapat disimpulkan bahwa ekstrak etanol daun kirinyuh memiliki aktivitas penghambatan terhadap enzim α-amilase dan glukoamilase.

Banyak kandungan senyawa kimia bermanfaat dalam makroalga yang bernilai ekonomi tinggi tetapi belum diteliti secara luas, seperti pigmen fotosintesis. Peningkatan  kebutuhan pigmen fotosintesis pada dunia industri menyebabkan pencarian sumber alternatif pigmen fotosintesis masih terus dilakukan diantaranya dari makroalga. Penelitian ini bertujuan untuk mengetahuipengaruh lama penyimpanan ekstrak kasar makroalga Ulva lactuca terhadap kestabilan pigmen fotosintesis. Tahapan penelitian meliputi, isolasi pigmen fotosintesis, pemurnian menggunakan kromatografi kolom silika, karakterisasi pigmen fotosintesis dengan KLT dan spektrofotometer UV–Vis. Hasil KLT dari ekstrak kasar Ulva lactuca menunjukkan perbedaan jumlah dan jenis pigmen fotosintesis yang mencolok antara ekstrak kasar Ulva lactuca yang lansung diekstrak dan yang dibiarkan kurang lebih 3 hari. Pada hasil kromarogram menunjukkan ada 7 fraksi pigmen fotosintesis dan memiliki warna yang sangat mencolok yang diekstrak secara langsung sedangkan ekstrak kasar yang dibiarkan kurang lebih 3 hari menunjukkan hanya 5 fraksi pigmen fotosintesis dengan warna yang pucat. Fraksinasi dengan kromatografi kolom silika gel menunjukkan adanya pigmen fotosintesis sebanyak 29 fraksi. Setelah diindentifikasi dengan spektofotometer UV–Vis ada 4 fraksi utama yaitu ß-karoten, fukoxantin, klorofil a,dan feofitin a. Lama penyimpanan terhadap ekstrak kasar Ulva lactuca mempengaruhi pigmen fotosintesis secara kualitatif dan kuantitatif.

In silico studies on interactions between the human pancreatic α-amylase (HPA) enzyme with α, β, and γ-mangostin ligands has been carried out using the molecular docking method. Ligands α, β, and γ-mangostin interact through the formation of hydrogen and van der waals bonds with residues on the enzyme active side. The α-mangostin ligands form seven hydrogen and six van der waals bonds with residues involved were Trp59, Gln63, Trp96, Thr163, Thr164, Ala198, His201, Glu233, and Asp300; β-mangostin forms five hydrogen and eight van der waals bonds with residues involved were Gln63, Trp96, Thr163, Thr164, Arg195, Asp197, His201, Glu233, Asp300, and His305; while γ-mangostin forms nine hydrogen and five van der waals bonds with residues involved were Trp59, Gln63, Trp96, Thr163, Asp197, Ala198, His201, Glu233, and Asp300. The binding afinity of α, β, and γ-mangostin to the HPA obtained were -7.0; -6.6; and -7.4 kcal/mol with RMSD value were 1,850; 1,956; and 1,811 Å, respectively. The number of hydrogen bonds that can be formed was responsible to the binding affinity. Ligand γ-mangostin has potential activity as an inhibitor of HPA enzyme due to the stable complexes formation with lower binding affinity (validated with RMSD value) when compared to α and β-mangostin.

Sagu tumbuh dan berkembang sangat banyak di daerah Maluku. Sagu memiliki potensi yang paling besar untuk digunakan sebagai pengganti beras. Keuntungan sagu dibandingkan dengan sumber karbohidrat lainnya adalah tanaman sagu atau hutan sagu sudah siap dipanen bila diinginkan dan memiliki nilai kandungan gizi dan ekonomis yang tinggi namun belum dikelola secara baik sesuai permintaan pasar. Oleh karena itu, melalui Program Produk Teknologi yang Diseminasikan ke Masyarakat diharapkan ada tranformasi learning yang diberikan untuk mengembangkan masyarakat khususnya di Desa Porto, Kecamatan Saparua, Kabupaten Maluku Tengah yang mempunyai usaha yang belum sesuai standar pasar. Metode pelaksanaan dilakukan melalui sosialisasi atau penyuluhan kepada mitra, memberikan keterampilan teknis pembuatan peralatan pengolahan sagu, dan pelatihan partisipatif. Sosialisasi dilakukan secara berkelompok dan penggalian solusi masalah dengan teknik Focus Group Discussion. Hasil yang didapat berupa penerapan teknologi tepat guna oleh mitra diantaranya pembuatan alat pemarut batang sagu termodifikasi yang mampu menghasilkan empulur sagu yang lebih halus, alat ekstraksi pati sagu termodifikasi yang dapat memaksimalkan proses ekstraksi sehingga sagu mentah yang dihasilkan lebih banyak, dan alat pemanggang pati sagu untuk pembuatan “Sagu Merah-Putih”. Masyarakat berharap kegiatan ini tidak berhenti sampai disini, tetapi ada bantuan untuk memfasilitasi pengadaan peralatan atau teknologi ini ke depan. Kata kunci: Desa Porto, Sagu, Teknologi, Produk Merah-Putih ABSTRACT Sago grows and develops well in Maluku region. Sago has the greatest potential as a rice substituted. Sago has more benefit if compared to other carbohydrate sources because it ready to harvested if we want, has high nutritional and economic value, but has not been properly managed according to the market demand. Therefore, through The Technology Product Program that is Disseminated to the Community, hopely that there will be a learning transformation provided to develop the community, especially in Porto Village, Saparua District, Central Moluccas Regency. Socialization was carried out in groups and the exploration of problem solving using the Focus Groups Discussion technique. The results obtained were application of appropriate technology by partners including production of the modified sago stem gratertoolthat capable to produced finer sago pith, a modified sago starch extraction tool that maximizes the extraction process so that more raw sago is produced, and a sago starch roaster tool for production of "SaguMerah-Putih" product. The community hopes that activity doesn’t stop here, but there is acollaborated to facilitated the procurement of this equipments or technologies in the future. Keywords: Porto Village, Sago, Technology, Merah-Putih Products