Aktivitas Nitrat Reduktase (ANR) Tanaman Jagung pada Pola Tumpangsari yang Diberi Serasah Jagung-Kedelai serta Biochar di Lahan Suboptimal Sidondo Sulawesi Tengah

Abstract

The aim of the study was to examine the relationship between nitrate reductase activity and N uptake, tissue N content, N uptake, and chlorophyll content of corn plants in intercropping patterns which are given corn-soybean litter and biochar. The use of plant litter and biochar derived from crop residues as a source of organic matter in different intercropping patterns affected nitrate reductase activity, tissue N content, N uptake, and chlorophyll content of maize. This experiment was carried out on sub-optimal Sidondo land, Central Sulawesi with the main limiting factors being the availability of water and alkaline soil pH (7.21) which were arranged in a divided plot design with five litter treatments as subplots and five cropping patterns as the main plot with three replicates. Parameters observed were N levels, plant tissue N uptake, chlorophyll, and nitrate reductase activity. Collected data were analyzed using Anova and Duncan's multiple random tests. The results showed that corn litter, soybean mixed corn + soybean litter and their biochar in different intercropping patterns affected nitrate reductase activity, nitrogen uptake, chlorophyll levels very significantly (P˂0.01), increased nitrate reductase activity by 9710.86 mol NO2- -1g-1hour-1 at the age of 55 DAP, while N uptake (2237 mg.kg-1), tissue N content (4.33%), and total chlorophyll content (10.61 mg.l-1) were the highest in the litter treatment, corn and soybeans as well as biochar in corn and soybean intercropping (1:2), and (2:4). Thus, the provision of corn and soybean litter and biochar in intercropping systems can increase nitrate reductase activity, N uptake, tissue N levels, and corn chlorophyll levels in the corn-soybean intercropping system.
 
 Keywords: chlorophyll, N content of corn plant tissue, nitrate reductase activity, N uptake

Pengukuran Aktivitas Nitrat Reduktase (ANR) Pada Tanaman Poaceae Secara In vivo

“…Salah satu enzim yang dapat diukur aktivitasnya secara in vivo adalah enzim nitrat reduktase. Pengukuran aktivitas enzim nitrat reduktase merupakan salah satu upaya untuk mengetahui bagaimana metabolisme pada tumbuhan (Ende et al, 2022).…”

“…Ketersediaan N dipengaruhi oleh penambahan bahan organik pada lahan pertanian dengan faktor pembatas utama adalah air, kandungan c-organik yang rendah, dan pH alkalis. Ketersediaan N juga dipengaruhi oleh sistem pertanaman, terutama pada sistem tumpangsari (Ende et al, 2022). Sebagai unsur hara utama bagi pertumbuhan tanaman, nitrogen pada umumnya sangat diperlukan untuk pembentukan atau pertumbuhan bagian vegetatif tanaman, seperti daun, batang, dan akar sehingga sangat membatasi pertumbuhan tanaman (Adhikari et al, 2021).…”