Alanyl-glutamine administration suppresses Th17 and reduces inflammatory reaction in dextran sulfate sodium-induced acute colitis

Hou, Yu-Chih; Liu, Jun Jen; Pai, Man Hui; Tsou, Sing-Hua; Yeh, Sung Ling

doi:10.1016/j.intimp.2013.05.004

Cited by 21 publications

(23 citation statements)

References 47 publications

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“…This observation is much difference from the previous reports that arginine or glutamine supplementation significantly lowers these cytokines production [8], [9], [23]. The explanation for this difference remains unknown; perhaps it is related to the high dose of arginine (1.5% in diet) or glutamine (2.0% in diet) supplementation.…”

Section: Discussioncontrasting

confidence: 71%

“…Previous study reported that DSS causes weight loss in mice [8]. Thus, this study also calculated the average weight gain (AWG) from day 1 to 12.…”

Section: Resultsmentioning

confidence: 99%

“…Besides that, compelling investigations have indicated that arginine or glutamine might be a good candidate for IBD treatment with low risk [6], [7]. For instance, post-treatment with alanyl-glutamine attenuates inflammatory response in dextran sulfate sodium (DSS)-induced colitis by suppressing Th17-associated cytokine expressions, reducing macrophage infiltration into the peritoneal cavity, and decreasing pro-inflammatory cytokine production in the colon [8]. Meanwhile, arginine supplementation improves the clinical parameters, including mortality, body weight and colon weight, and lowers the colonic permeability and the number of myeloperoxidase-positive neutrophils in colitis induced by DSS [9].…”

Section: Introductionmentioning

confidence: 99%

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Serum Amino Acids Profile and the Beneficial Effects of L-Arginine or L-Glutamine Supplementation in Dextran Sulfate Sodium Colitis

et al. 2014

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This study was conducted to investigate serum amino acids profile in dextran sulfate sodium (DSS)-induced colitis, and impacts of graded dose of arginine or glutamine supplementation on the colitis. Using DSS-induced colitis model, which is similar to human ulcerative colitis, we determined serum profile of amino acids at day 3, 7, 10 and 12 (5 days post DSS treatment). Meanwhile, effects of graded dose of arginine (0.4%, 0.8%, and 1.5%) or glutamine (0.5%, 1.0% and 2.0%) supplementation on clinical parameters, serum amino acids, colonic tight junction proteins, colonic anti-oxidative indicators [catalase, total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px)], colonic pro-inflammatory cytokines [interleukin-1 beta (IL-1β), IL-6, IL-17 and tumor necrosis factor alpha (TNF-α)] in DSS-induced colitis were fully analyzed at day 7 and 12. Additionally, the activation of signal transduction pathways, including nuclear factor kappa B (NF-κB), mitogen-activated protein kinases (MAPK), phosphoinositide-3-kinases (PI3K)/PI3K-protein kinase B (Akt), and myosin light chain kinase (MLCK)- myosin light chain (MLC20), were analyzed using immunoblotting. Serum amino acids analysis showed that DSS treatment changed the serum contents of amino acids, such as Trp, Glu, and Gln (P<0.05). Dietary arginine or glutamine supplementation had significant (P<0.05) influence on the clinical and biochemical parameters (T-SOD, IL-17 and TNF-α) in colitis model. These results were associated with colonic NF-κB, PI3K-Akt and MLCK signaling pathways. In conclusion, arginine or glutamine could be a potential therapy for intestinal inflammatory diseases.

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Section: Discussioncontrasting

confidence: 71%

“…Previous study reported that DSS causes weight loss in mice [8]. Thus, this study also calculated the average weight gain (AWG) from day 1 to 12.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Serum Amino Acids Profile and the Beneficial Effects of L-Arginine or L-Glutamine Supplementation in Dextran Sulfate Sodium Colitis

et al. 2014

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“…Therefore bioactive peptides have the potential to be used as an alternative therapy for IBD and other chronic inflammatory disorders. According to Hou et al[ 106 ], treatment with alanyl-glutamine in a mouse model of colitis suppressed Th-17 cytokines and macrophage migration to the peritoneal cavity, indicating a reduction in the inflammatory response. Propionyl-L-carnitine, an essential factor of transporting fatty acids in mitochondria reduced mucosal inflammation through antioxidative effects in TNBS-induced colitis[ 107 ].…”

Section: Functional Foods and Nutraceuticals For Ibdmentioning

confidence: 99%

Diets, functional foods, and nutraceuticals as alternative therapies for inflammatory bowel disease: Present status and future trends

Mijan

Lim

2018

WJG

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Inflammatory bowel disease (IBD) is a serious health concern among western societies. The disease is also on the rise in some East Asian countries and in Australia. Health professionals and dietitians around the world are facing an unprecedented challenge to prevent and control the increasing prevalence of IBD. The current therapeutic strategy that includes drugs and biological treatments is inefficient and are associated with adverse health consequences. In this context, the use of natural products is gaining worldwide attention. In vivo studies and clinical evidence suggest that well-planned dietary regimens with specific nutrients can alleviate gastrointestinal inflammation by modulating inflammatory cytokines, such as tumor necrosis factor α (TNF-α), interleukin 1 (IL-1), IL-6, IL-1β, and IL-10. Alternatively, the avoidance of high-fat and high-carbohydrate diets is regarded as an effective tool to eliminate the causes of IBD. Many functional foods and bioactive components have received attention for showing strong therapeutic effects against IBD. Both animal and human studies suggest that bioactive functional foods can ameliorate IBD by downregulating the pro-inflammatory signaling pathways, such as nuclear factor κB, STAT1, STAT6, and pro-inflammatory cytokines, including IL-1β, IL-4, IL-6, COX-2, TNF-α, and interferon γ. Therefore, functional foods and diets have the potential to alleviate IBD by modulating the underlying pathogenic mechanisms. Future comprehensive studies are needed to corroborate the potential roles of functional foods and diets in the prevention and control of IBD.

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“…In mice, IL-17 is essential for host defense against S . aureus infections of the skin [ 21 ] and inhibition of IL-17 is associated with development of acute colitis after exposure to dextran sulfate sodium [ 22 ]. In humans, reduced levels of Th17 cells lead to increased susceptibility to S .…”

Section: Discussionmentioning

confidence: 99%

Human genetic variation in GLS2 is associated with development of complicated Staphylococcus aureus bacteremia

et al. 2018

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The role of host genetic variation in the development of complicated Staphylococcus aureus bacteremia (SAB) is poorly understood. We used whole exome sequencing (WES) to examine the cumulative effect of coding variants in each gene on risk of complicated SAB in a discovery sample of 168 SAB cases (84 complicated and 84 uncomplicated, frequency matched by age, sex, and bacterial clonal complex [CC]), and then evaluated the most significantly associated genes in a replication sample of 240 SAB cases (122 complicated and 118 uncomplicated, frequency matched for age, sex, and CC) using targeted sequence capture. In the discovery sample, gene-based analysis using the SKAT-O program identified 334 genes associated with complicated SAB at p<3.5 x 10−3. These, along with eight biologically relevant candidate genes were examined in the replication sample. Gene-based analysis of the 342 genes in the replication sample using SKAT-O identified one gene, GLS2, significantly associated with complicated SAB (p = 1.2 x 10−4) after Bonferroni correction. In Firth-bias corrected logistic regression analysis of individual variants, the strongest association across all 10,931 variants in the replication sample was with rs2657878 in GLS2 (p = 5 x 10−4). This variant is strongly correlated with a missense variant (rs2657879, p = 4.4 x 10−3) in which the minor allele (associated here with complicated SAB) has been previously associated with lower plasma concentration of glutamine. In a microarray-based gene-expression analysis, individuals with SAB exhibited significantly lower expression levels of GLS2 than healthy controls. Similarly, Gls2 expression is lower in response to S. aureus exposure in mouse RAW 264.7 macrophage cells. Compared to wild-type cells, RAW 264.7 cells with Gls2 silenced by CRISPR-Cas9 genome editing have decreased IL1-β transcription and increased nitric oxide production after S. aureus exposure. GLS2 is an interesting candidate gene for complicated SAB due to its role in regulating glutamine metabolism, a key factor in leukocyte activation.

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Alanyl-glutamine administration suppresses Th17 and reduces inflammatory reaction in dextran sulfate sodium-induced acute colitis

Cited by 21 publications

References 47 publications

Serum Amino Acids Profile and the Beneficial Effects of L-Arginine or L-Glutamine Supplementation in Dextran Sulfate Sodium Colitis

Serum Amino Acids Profile and the Beneficial Effects of L-Arginine or L-Glutamine Supplementation in Dextran Sulfate Sodium Colitis

Diets, functional foods, and nutraceuticals as alternative therapies for inflammatory bowel disease: Present status and future trends

Human genetic variation in GLS2 is associated with development of complicated Staphylococcus aureus bacteremia

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