Serum Amino Acids Profile and the Beneficial Effects of L-Arginine or L-Glutamine Supplementation in Dextran Sulfate Sodium Colitis

Ren, Wenkai; Yin, Jie; Wu, Miaomiao; Liu, Gang; Yang, Guan; Xion, Yan; Su, Dingding; Wu, Li; Li, Tiejun; Chen, Shuai; Duan, Jielin; Yin, Yulong; Wu, Guoyao

doi:10.1371/journal.pone.0088335

Cited by 140 publications

(133 citation statements)

References 37 publications

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“…The mice in groups (i) and (ii) were given a basic diet and ready access to clean drinking water for 7 days, whereas the mice in groups (iii) and (iv) received a basic diet with drinking water containing 30 mg/ml IRW or IWQ for the same period of time. Commencing on day 7, all of the mice, except for those in group (i), were given ready access to drinking water containing 5% DSS solution for a further 7 days [17]. This drinking water was prepared by dissolving DSS (molecular weight 5, 000, supplied by Kayon Biological Technology Co. Ltd.) in tap water to obtain a 5% (wt/vol) DSS solution.…”

Section: Experimental Designmentioning

confidence: 99%

“…This drinking water was prepared by dissolving DSS (molecular weight 5, 000, supplied by Kayon Biological Technology Co. Ltd.) in tap water to obtain a 5% (wt/vol) DSS solution. On the morning of day 15 (the end of the experimental period), each mouse was weighed to assess the average daily weight gain and then each mouse was sacrificed and colon length and weight were measured as detailed in our previous publication [17]. Colon tissues and contents from each mouse were also collected, immediately frozen in liquid nitrogen and retained at −70 °C for further analysis.…”

Section: Experimental Designmentioning

confidence: 99%

“…In addition to this anti-inflammatory effect, the observed advantages of these peptides have included antioxidant effects [16]. The capacity for dietary constituents to function as modulators for intestinal inflammatory disease has been demonstrated by previous work examining nutritional regulation by amino acids [17][18][19]. This study examines the impacts of the presence of dietary IRW or IQW on oxidative stress and colon microbiota in dextran sodium sulfate (DSS) induced colitis.…”

Section: Introductionmentioning

confidence: 99%

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Effects of IRW and IQW on Oxidative Stress and Gut Microbiota in Dextran Sodium Sulfate-Induced Colitis

Liu

Yan

Ding

et al. 2018

Cell Physiol Biochem

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Background/Aims: There are known links between inflammatory bowel disease (IBD) and changes in the microbiota of the gut and inflammation and oxidative stress. In this study, a colitis model induced by dextran sodium sulfate (DSS) in mice is used to evaluate whether the presence of bioactive peptides IRW (Ile-Arg-Trp) and IQW (Ile-Gln-Trp) peptides is advantageous. Methods: The mice were arbitrarily assigned to the following four groups: (i) control (untreated), (ii) dextran sodium sulfate (DSS) treated, (iii) IRW-DSS treated, and (iv) IQW-DSS treated. For 7 days, the control group subjects had unrestricted access to untreated drinking water, whereas the drinking water supplied to the subjects in the DSS, IRW-DSS, and IQW-DSS groups during this period consisted of 5% DSS solution. The colonic lesions were scored after hematoxylin and eosin staining. Serum antioxidant capacity was analyzed by 2,2’-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS) radical cation decolorization test and the microbiota in the colonic contents were sequenced by HiSeq2500 PE250. Results: The presence of DSS reduced daily weight gain, enhanced histopathology scores, and inhibited antioxidant enzyme expression. Superoxide dismutase, catalase, and glutathione peroxidase activities in the DSS-induced colitis model were significantly enhanced (P < 0.05) in the presence of dietary IRW and IQW. Furthermore, the Simpson index was significantly increased (P < 0.05) in the presence of dietary IRW and IQW compared to the control group. IRW and IQW increased the abundance of Coprococcus_1, Ruminococcaceae_UCG-014, and Desulfovibrio compared to the control group and DSS group. Furthermore, IQW decreased the abundance of Bacteroides in relation to the control group, but increased Parabacteroides. In addition, IRW increased the level of Anaerotruncus, Oscillibacter, and Ruminiclostridium_9 compared to the control group. Conclusion: This study concludes that the presence of IRW or IQW can mitigate DSS-induced oxidative stress by improving the activities of antioxidant enzymes, increasing intestinal microbial diversity and enhancing the abundance of gut microbiota, which may help maintain the homeostasis of host health and microenvironment in a DSS-induced mouse model, thus providing a potential further treatment for IBD patients.

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Section: Experimental Designmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of IRW and IQW on Oxidative Stress and Gut Microbiota in Dextran Sodium Sulfate-Induced Colitis

Liu

Yan

Ding

et al. 2018

Cell Physiol Biochem

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“…Briefly, 1 mL of the plasma sample and 2.5 mL of 7.5 % trichloroacetic acid solution were mixed thoroughly and centrifuged at 12,000×g and 4 °C for 15 min (Ren et al 2014c). The supernatant fluid was collected for analysis of free AAs by an ion-exchange AA analyzer (L8800, Hitachi, Tokyo, Japan).…”

Section: Determination Of Free Amino Acids In Plasmamentioning

confidence: 99%

Co-dependence of genotype and dietary protein intake to affect expression on amino acid/peptide transporters in porcine skeletal muscle

Liu

Kong

et al. 2015

Amino Acids

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A total of 96 barrows (48 pure-bred Bama mini-pigs representing fatty genotype, and 48 Landrace pigs representing lean genotype) were randomly assigned to either a low- or adequate-protein treatment diet. The experimental period commenced at 5 weeks of age and extended to the finishing period. After euthanasia, blood and skeletal muscle samples were collected from pigs at the nursery, growing, and finishing phases. Our results indicate that the concentrations of free AAs in the plasma and muscle decreased as the age of the pigs increased. In addition, a strain × growth phase interaction (P < 0.05) was observed for the free AA pool in the plasma and muscle. The low-protein diet upregulated (P < 0.05) the mRNA levels for T1R1/T1R3 involved in glutamate binding, but downregulated (P < 0.05) the mRNA levels for PAT1, PAT2, and ASCT2, which transport neutral AAs into muscles. Bama mini-pigs had higher (P < 0.05) mRNA levels for LAT1, SNAT2, and EAAC1, but a lower (P < 0.05) mRNA level for PepT1, compared with Landrace pigs. Collectively, our findings indicate that adequate provision of dietary protein plays an important role in regulating profiles of free AA pools and expression of key AA/peptide transporters/transceptors in a genotype- and tissue-specific manner.

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“…Therefore, we measured the levels of key antioxidant enzymes, catalase, GPx1, and SOD1, which are shown to be reduced in models of IBD (Cetinkaya et al, 2006;Sakthivel and Guruvayoorappan, 2013;Ren et al, 2014). Figure 5A shows that the level of each enzyme increases maximally when Clamidine is dosed at 5 mg/ml (12 mM) in ANA-1 murine macrophages.…”

Section: Resultsmentioning

confidence: 99%

Inhibiting Protein Arginine Deiminases Has Antioxidant Consequences

Witalison

Cui

Hofseth

et al. 2015

J Pharmacol Exp Ther

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Ulcerative colitis is a dynamic, idiopathic, chronic inflammatory condition that carries a high colon cancer risk. We previously showed that Cl-amidine, a small-molecule inhibitor of the protein arginine deiminases, suppresses colitis in mice. Because colitis is defined as inflammation of the colon associated with infiltration of white blood cells that release free radicals and citrullination is an inflammationdependent process, we asked whether Cl-amidine has antioxidant properties. Here we show that colitis induced with azoxymethane via intraperitoneal injection 1 2% dextran sulfate sodium in the drinking water is suppressed by Cl-amidine (also given in the drinking water).Inducible nitric oxide synthase, an inflammatory marker, was also downregulated in macrophages by Cl-amidine. Because epithelial cell DNA damage associated with colitis is at least in part a result of an oxidative burst from overactive leukocytes, we tested the hypothesis that Cl-amidine can inhibit leukocyte activation, as well as subsequent target epithelial cell DNA damage in vitro and in vivo. Results are consistent with this hypothesis, and because DNA damage is a procancerous mechanism, our data predict that Clamidine will not only suppress colitis, but we hypothesize that it may prevent colon cancer associated with colitis.

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Serum Amino Acids Profile and the Beneficial Effects of L-Arginine or L-Glutamine Supplementation in Dextran Sulfate Sodium Colitis

Cited by 140 publications

References 37 publications

Effects of IRW and IQW on Oxidative Stress and Gut Microbiota in Dextran Sodium Sulfate-Induced Colitis

Effects of IRW and IQW on Oxidative Stress and Gut Microbiota in Dextran Sodium Sulfate-Induced Colitis

Co-dependence of genotype and dietary protein intake to affect expression on amino acid/peptide transporters in porcine skeletal muscle

Inhibiting Protein Arginine Deiminases Has Antioxidant Consequences

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