Albumin and Globulin Proteins of Wheat Flour: Immunological and N-terminal Sequence Characterisation

Singh, Jaswinder; Blundell, Malcolm; Tanner, Gregory J.; Skerritt, John H.

doi:10.1006/jcrs.2001.0380

Cited by 36 publications

(28 citation statements)

References 30 publications

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“…Spot 3 corresponds to the cleavage of proglobulin-3A at cleavage site 3, with predicted M r 28.6 kDa and pI 5.76. The processing of Spot 4 is supported by N-terminal sequence data that matches the previously documented cleavage site 2 (XXHGDSRR) [26]. Spot 4 corresponds to cleavage of proglobulin-3A at cleavage sites 2 and 4, with predicted M r 35.1 kDa and pI 9.14.…”

Section: Resultssupporting

confidence: 73%

“…These findings suggest that these ~30 kDa globulin-3-related polypeptides could arise from different processing events of the same precursor, as was observed for barley Beg1 [9,35]. Curiously, there were no proteins identified in the immunoblotting experiments that corresponded to the product of processing at cleavage site 4, as was documented in other studies that sequenced globulin-3 (Figure 3 and [19,20,26]). Potentially, as the anti‐globulin-3A antibodies are polyclonal and were prepared by the co-immunization of rabbits with a mixture of two separate peptides [45], it is possible that the antibodies recognize only one of the two peptides.…”

Section: Discussionsupporting

confidence: 68%

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Seed storage proteins of the globulin family are cleaved post-translationally in wheat embryos

et al. 2012

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BackgroundThe 7S globulins are plant seed storage proteins that have been associated with the development of a number of human diseases, including peanut allergy. Immune reactivity to the wheat seed storage protein globulin-3 (Glo-3) has been associated with the development of the autoimmune disease type 1 diabetes in diabetes-prone rats and mice, as well as in a subset of human patients.FindingsThe present study characterized native wheat Glo-3 in salt-soluble wheat seed protein extracts. Glo-3-like peptides were observed primarily in the wheat embryo. Glo-3-like proteins varied significantly in their molecular masses and isoelectric points, as determined by two dimensional electrophoresis and immunoblotting with anti-Glo-3A antibodies. Five major polypeptide spots were identified by mass spectrometry and N-terminal sequencing as belonging to the Glo-3 family.ConclusionsThese results in combination with our previous findings have allowed for the development of a hypothetical model of the post-translational events contributing to the wheat 7S globulin profile in mature wheat kernels.

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Section: Resultssupporting

confidence: 73%

Section: Discussionsupporting

confidence: 68%

Seed storage proteins of the globulin family are cleaved post-translationally in wheat embryos

et al. 2012

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“…Analysis of sequence coverage indicated that these spots were produced by cleavage of the N-termini of these proteins. These results are consistent with previous investigations indicating that globulin-3 family proteins can be processed at internal cleavage sites to yield polypeptides with a large range of molecular weights and pI values (Singh et al 2001;Dupont et al 2011;Koziol et al 2012). Interestingly, the spots corresponding to full-length globulin were downaccumulated under e[CO 2 ], whereas those corresponding to endo-proteolytic events accumulated or appeared in samples grown under these conditions.…”

Section: Changes In Seed Proteome Profilessupporting

confidence: 92%

Elevated field atmospheric CO2 concentrations affect the characteristics of winter wheat (cv. Bologna) grains

Verrillo

Badeck²,

Terzi³

et al. 2017

Crop Pasture Sci.

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Abstract. The aim of this study was to investigate the impact of elevated concentration of carbon dioxide (CO 2 ), as expected over coming decades, on yield and quality of winter bread wheat (Triticum aestivum L.). Plants (cv. Bologna) were grown by using the free-air CO 2 enrichment (FACE) system at Fiorenzuola d'Arda under ambient (control) and elevated (570 ppm, e[CO 2 ]) CO 2 concentrations for two growing seasons. We addressed whether there would be a response of wheat grains to elevated CO 2 concentration in terms of the contents of nitrogen (N), micro-and macronutrients, proteins and free amino acids. Under e[CO 2 ], total wheat biomass and grain yield increased in both years of the study. Grain N percentage was reduced under e[CO 2 ], but grain N yield (kg ha -1 ) was increased. Among macro-and micronutrients, a decrease in zinc concentration was observed. The proteome pattern was significantly different in grains grown at the two different CO 2 levels, but the observed changes were highly dependent on interactions with prevailing environmental conditions. Finally, a negative trend was observed in the early germination rates of seeds from plants grown under e[CO 2 ] compared with the controls. The results suggest that the expected increase in CO 2 levels and their interactive effects with environmental variables may influence agronomic performance by increasing yield and negatively affecting quality.

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“…Variation of subunits between gliadin and glutenin proteins in mealy/vitreous kernels can affect the Gli-to-Glu ratio, which is an important indicator of grain technological/baking quality. In our study it varied between cultivar samples from 1.08 to 1.31, and was similar to that presented by Singh et al (2001), Shewry and Halford (2002), Konopka et al (2007), and Gil-Humanes et al (2012). According to Samson et al (2005), the Gli-to-Glu ratio of vitreous endosperm in durum wheat was higher than 0.85 in more than 98% of the samples.…”

Section: Discussionsupporting

confidence: 48%

Differences of some chemicals and physical properties of winter wheat grain of mealy and vitreous appearance

Konopka

Tańska

Konopka

2015

Cereal Research Communications

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Many wheat species and cultivars, independent of genetic markers of hardness, can produce grain with a vitreous, mealy or mixed appearance. This study analyzed selected chemical and physical differences between kernels with a vitreous and mealy appearance, hand-picked from grain of four winter wheat cultivars cultivated in Poland. Separated fractions were examined for protein content and composition, friabilin presence, carotenoids and total phenolic compounds content, specific kernel density, hardness, as well as kernel surface color. It was found that the ratio of vitreous kernels in the cultivars ranged from 39.18% to 76.28%. Vitreous kernels were darker, slightly heavier and harder than mealy kernels. Additionally, these kernels were more abundant in proteins (an average increase of 2.13%, with variation among cultivars from 0.71% to 2.89%). This type of kernels was also richer in phenolic compounds (on average by 4.02%) and less abundant in carotenoids (on average by 4.53%). Mealy (softer) kernels fractured to a finer flour.Keywords: endosperm vitreousness, mealiness, gluten proteins, kernel density, hardness IntroductionWheat cultivars produce grain in which fully vitreous and mealy, or kernels with mixed endosperm structure exist (Horrobin et al. 2003). So-called "piebald" or "yellow berry" kernels can occur in both durum and hexaploid wheat, with mealy and vitreous zones in a sharply-defined area (Dexter et al. 1989). Evers and Bechtel (1988) explained mealy (starchy, chalky, opaque, soft) endosperm as a result of light scattering at the air-starch and air-protein interfaces. Later studies showed that vitreousness is related to the endosperm microstructure (Greffeuille et al. 2006) as well as to kernel color and its mechanical properties (Peterson et al. 2001;Konopka et al. 2005). Soft endosperm is usually linked with a chalky-opaque appearance, and hard endosperm is linked with a glassy-vitreous appearance. Although the vitreousness of endosperm tissue is more typical of hard wheat, 0133-3720/$20.00 © 2015 Akadémiai Kiadó, Budapest * Corresponding author; E-mail: m.tanska@uwm.edu.pl Cereal Research Communications 43(3), pp. 470-480 (2015) DOI: 10.1556/CRC.2014.0048 First published online 21 April 2015 soft wheat cultivars may also produce cohesive hard sections. Morris et al. (2008) found large variations in endosperm microstructure from kernel-to-kernel within pure cultivars of soft, hard and durum wheats. Such variability can also be affected by the vegetation conditions, especially by fertilization (Samson et al. 2005) and climate (Evers and Bechtel 1988). For example, when a soft cultivar of wheat is grown under optimum conditions, it produces vitreous kernels and, in contrast, many hard types of wheat produce opaque/mealy kernels (Pasha et al. 2010). Kernel vitreousness has been found to generally increase with nitrogen fertilization supply (Farm Facts 1997) and can be declined by damp conditions before grain harvest (Sandhu et al. 2009).It was initially thought that starchy and vitreous zone...

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Albumin and Globulin Proteins of Wheat Flour: Immunological and N-terminal Sequence Characterisation

Cited by 36 publications

References 30 publications

Seed storage proteins of the globulin family are cleaved post-translationally in wheat embryos

Seed storage proteins of the globulin family are cleaved post-translationally in wheat embryos

Elevated field atmospheric CO2 concentrations affect the characteristics of winter wheat (cv. Bologna) grains

Differences of some chemicals and physical properties of winter wheat grain of mealy and vitreous appearance

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