Albumin and hemoglobin adducts as biomarkers of exposure to styrene in fiberglass-reinforced-plastics workers

Fustinoni, Silvia; Colosio, Claudio; Colombi, A; Lastrucci, Lorenzo; Yeowell-O'Connell, Karen; Sm, Rappaport

doi:10.1007/s004200050247

Cited by 31 publications

(23 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, no effects were observed for the corresponding adducts of Hb, and none of the protein adducts was significantly associated with individual exposure to Sty or StyOX among exposed workers (Table V). Furthermore, we observed high levels of background protein adducts as previously reported in other studies , Fustinoni et al 1998. Such background adducts tends to obscure exposureÁadduct relationships unless large numbers of subjects are investigated.…”

Section: Discussionsupporting

confidence: 66%

“…Cysteinyl adducts of StyOX with Alb and Hb were assayed as described previously , Fustinoni et al 1998). These adducts were detected as 1-phenylethanol (1PE) and 2-phenylethanol (2PE), representing substitution at the a-and b-carbons of StyOX, respectively, and are designated as 1PE-Alb, 2PE-Alb, 1PE-Hb and 2PE-Hb.…”

Section: Analysis Of Air and Biological Samplesmentioning

confidence: 99%

“…In one study, levels of StyOX-Alb were significantly correlated with exposures to both Sty and StyOX, as well as with StyOX adducts of DNA, and sister chromatid exchanges . However, the presence of significant background levels of StyOX-Alb and StyOX-Hb (in subjects without occupational exposures) has limited the utility of these adducts as biomarkers of exposure , Fustinoni, et al 1998). …”

Section: Introductionmentioning

confidence: 98%

“…The few attempts at biomonitoring of StyOX exposures have focused upon direct measurements of StyOX in blood (Korn et al 1994, Tornero-Velez et al 2001b, Serdar et al 2006 or measurements of adducts of StyOX with the blood proteins haemoglobin (Hb) and Alb , Fustinoni et al 1998. Of these options, measurements of StyOX-Hb or StyOX-Alb are generally preferred because they are stable and accumulate in humans over 1 or 2 months (Granath et al 1992), whereas StyOX is unstable in blood (Yeowell-O'Connell et al 1997).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

An integrated approach to biomonitoring exposure to styrene and styrene-(7,8)-oxide using a repeated measurements sampling design

et al. 2008

Self Cite

View full text Add to dashboard Cite

The aim of this work was to investigate urinary analytes and haemoglobin and albumin adducts as biomarkers of exposure to airborne styrene (Sty) and styrene-(7,8)-oxide (StyOX) and to evaluate the influence of smoking habit and genetic polymorphism of metabolic enzymes GSTM1 and GSTT1 on these biomarkers. We obtained three or four air and urine samples from each exposed worker (eight reinforced plastics workers and 13 varnish workers), one air and urine samples from 22 control workers (automobile mechanics) and one blood sample from all subjects. Median levels of exposure to Sty and StyOX, respectively, were 18.2 mg m(-3) and 133 microg m(-3) for reinforced plastics workers, 3.4 mg m(-3) and 12 microg m(-3) for varnish workers, and <0.3 mg m(-3) and <5 microg m(-3) for controls. Urinary levels of styrene, mandelic acid, phenylglyoxylic acid, phenylglycine (PHG), 4-vinylphenol (VP) and mercapturic acids (M1+M2), as well as cysteinyl adducts of serum albumin (but not those of haemoglobin) were significantly associated with exposure status (controls

show abstract

Section: Discussionsupporting

confidence: 66%

Section: Analysis Of Air and Biological Samplesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

An integrated approach to biomonitoring exposure to styrene and styrene-(7,8)-oxide using a repeated measurements sampling design

et al. 2008

Self Cite

View full text Add to dashboard Cite

show abstract

“…In fact, measurable levels of adducts of butadiene (18-60 nmol/g globin) (Bagemann et al, 2001); nmol/g globin of THB-Val (Fustinoni et al, 2002) and of styrene (sum of 1-and 2-PE adducts: 2-9 nmol/g globin; 1.5-6 nmol/g albumin) (Fustinoni et al, 1998) have been measured even in some non-smoking, occupationally unexposed control subjects. However, in those cases no comparably reliable study or reasonably grounded hypothesis is currently available on possible biological sources in humans not entailing exposure to the parent chemicals.…”

Section: Reaction With Epoxidesmentioning

confidence: 97%

Toward an “omic” physiopathology of reactive chemicals: Thirty years of mass spectrometric study of the protein adducts with endogenous and xenobiotic compounds

Rubino

Pitton

Fabio

et al. 2009

Mass Spectrometry Reviews

109

170

View full text Add to dashboard Cite

Cancer and degenerative diseases are major causes of morbidity and death, derived from the permanent modification of key biopolymers such as DNA and regulatory proteins by usually smaller, reactive molecules, present in the environment or generated from endogenous and xenobiotic components by the body's own biochemical mechanisms (molecular adducts). In particular, protein adducts with organic electrophiles have been studied for more than 30 [see, e.g., Calleman et al., 1978] years essentially for three purposes: (a) as passive monitors of the mean level of individual exposure to specific chemicals, either endogenously present in the human body or to which the subject is exposed through food or environmental contamination; (b) as quantitative indicators of the mean extent of the individual metabolic processing which converts a non-reactive chemical substance into its toxic products able to damage DNA (en route to cancer induction through genotoxic mechanisms) or key proteins (as in the case of several drugs, pesticides or otherwise biologically active substances); (c) to relate the extent of protein modification to that of biological function impairment (such as enzyme inhibition) finally causing the specific health damage. This review describes the role that contemporary mass spectrometry-based approaches employed in the qualitative and quantitative study of protein-electrophile adducts play in the discovery of the (bio)chemical mechanisms of toxic substances and highlights the future directions of research in this field. A particular emphasis is given to the measurement of often high levels of the protein adducts of several industrial and environmental pollutants in unexposed human populations, a phenomenon which highlights the possibility that a number of small organic molecules are generated in the human organism through minor metabolic processes, the imbalance of which may be the cause of "spontaneous" cases of cancer and of other degenerative diseases of still uncharacterized etiology. With all this in mind, it is foreseen that a holistic description of cellular functions will take advantage of new analytical methods based on time-integrated metabolomic measurements of a new biological compartment, the "adductome," aimed at better understanding integrated organism response to environmental and endogenous stressors.

show abstract

Epoxy Compounds—Olefin Oxides (Ethylene Oxide, Propylene Oxide, and Miscellaneous Oxides)

Klapacz

Doskey

2023

Patty's Toxicology

View full text Add to dashboard Cite

This chapter discusses the chemistry, uses, health information, and industrial hygiene handling procedures of epoxides, that is, substances containing strained, three‐membered rings with two adjacent carbons and one oxygen atom. This functionality underlies epoxides' high reactivity, physicochemical properties, and toxicity. The majority of the content is dedicated to ethylene oxide and propylene oxide, but other epoxides are also of industrial interest and are described here; their databases have evolved over the last couple of decades and this new understanding is highlighted here. These are 1,2‐butylene oxide, 2,3‐epoxybutane, butadiene diepoxide, vinylcyclohexene monoxide, vinylcyclohexene dioxide, and styrene oxide.

show abstract

Albumin and hemoglobin adducts as biomarkers of exposure to styrene in fiberglass-reinforced-plastics workers

Cited by 31 publications

References 20 publications

An integrated approach to biomonitoring exposure to styrene and styrene-(7,8)-oxide using a repeated measurements sampling design

An integrated approach to biomonitoring exposure to styrene and styrene-(7,8)-oxide using a repeated measurements sampling design

Toward an “omic” physiopathology of reactive chemicals: Thirty years of mass spectrometric study of the protein adducts with endogenous and xenobiotic compounds

Epoxy Compounds—Olefin Oxides (Ethylene Oxide, Propylene Oxide, and Miscellaneous Oxides)

Contact Info

Product

Resources

About