Albumin‐based hydrogels for regenerative engineering and cell transplantation

Ong, John; Zhao, Junzhe; Justin, Alexander W.; Markaki, Athina E.

doi:10.1002/bit.27167

Cited by 75 publications

(59 citation statements)

References 51 publications

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“…Nominal stresses (σ) and strains (ε) were plotted, and a line was least square fitted to the data points, which gave an apparent E in compression. 4 www.nature.com/scientificreports/ inactivated 10% FBS (Invitrogen, 10108-157), 1% Antibiotic-Antimycotic (Gibco, 15240062), 50 mg/ml L-Ascorbic Acid Phosphate and Magnesium Salt (Fujifilm Wako Chemical corporation, 013-19641). As a comparison for immuno-staining, clinical grade human embryonic stem cell (hESC) line KCL-033, a kind gift from Dr Tamir Rashid (King's College London), was used.…”

Section: Methodsmentioning

confidence: 99%

“…These desirable attributes have led to extensive research into albumin as a protein conjugate for drug delivery and pharmacotherapy 1 – 3 . However, the use of albumin-based hydrogels in biomedical research is comparatively under-studied 4 .…”

Section: Introductionmentioning

confidence: 99%

“…gelation precipitated by strongly acidic or strongly alkali pH produces transparent albumin hydrogels whereas heat induced gelation produces an opaque hydrogel with larger pore sizes and a longer biodegradation time 6,10 . The most commonly used method is chemical crosslinking, often with glutaraldehyde or conjugated with functionalised polyethylene glycol (PEG) 4 . However these processes are laborious, costly and the resulting scaffolds can be immunogenic 4,11 .…”

mentioning

confidence: 99%

“…The most commonly used method is chemical crosslinking, often with glutaraldehyde or conjugated with functionalised polyethylene glycol (PEG) 4 . However these processes are laborious, costly and the resulting scaffolds can be immunogenic 4,11 . Heat-induced gelation is by far the easiest method to synthesize albumin hydrogels, however the opacity of resulting hydrogels precludes normal microscopy often required for cell and tissue culture.…”

mentioning

confidence: 99%

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Functionalisation of a heat-derived and bio-inert albumin hydrogel with extracellular matrix by air plasma treatment

Ong

Zhao

Levy

et al. 2020

Sci Rep

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Albumin-based hydrogels are increasingly attractive in tissue engineering because they provide a xeno-free, biocompatible and potentially patient-specific platform for tissue engineering and drug delivery. The majority of research on albumin hydrogels has focused on bovine serum albumin (BSA), leaving human serum albumin (HSA) comparatively understudied. Different gelation methods are usually employed for HSA and BSA, and variations in the amino acid sequences of HSA and BSA exist; these account for differences in the hydrogel properties. Heat-induced gelation of aqueous HSA is the easiest method of synthesizing HSA hydrogels however hydrogel opacity and poor cell attachment limit their usefulness in downstream applications. Here, a solution to this problem is presented. Stable and translucent HSA hydrogels were created by controlled thermal gelation and the addition of sodium chloride. the resulting bio-inert hydrogel was then subjected to air plasma treatment which functionalised its surface, enabling the attachment of basement membrane matrix (Geltrex). In vitro survival and proliferation studies of foetal human osteoblasts subsequently demonstrated good biocompatibility of functionalised albumin hydrogels compared to untreated samples. Thus, air plasma treatment enables functionalisation of inert heat-derived HSA hydrogels with extracellular matrix proteins and these may be used as a xeno-free platform for biomedical research or cell therapy. Albumin is an abundant non-glycosylated, 66.4 kDa protein in human serum that has a physiological half-life of approximately 19 days. It is synthesized predominantly by hepatocytes and poorly excreted through the renal glomerulus 1. Being poorly metabolised and weakly immunogenic, it is stable in vivo. Albumin is also versatile, acting as a weak pH buffer, and as a stabiliser to important proteins, hormones, metal ions, nanoparticles and drugs, making it an attractive biomaterial. These desirable attributes have led to extensive research into albumin as a protein conjugate for drug delivery and pharmacotherapy 1-3. However, the use of albumin-based hydrogels in biomedical research is comparatively under-studied 4. Recently, we demonstrated that human serum albumin (HSA) embedded in a fibrin hydrogel significantly promoted osteoblast differentiation and vascular self-organisation of human endothelial cells 5. However, high cell numbers (10 6 cells/ml or greater) degrade these fibrin-based hydrogels within 8-10 days making it unsuitable for studying long term bone development and implantation into animal models. Furthermore, it was anticipated that the implantation of fibrin-based scaffolds into bone would accelerate its degradation in vivo because of the presence of serum fibrinolytics and movement of bone during locomotion. Therefore, albumin-based hydrogels were explored as an alternative material for scaffolds. Heat-derived bovine serum albumin (BSA) hydrogels (20% w/v) exhibit high Young's modulus (~ 55 kPa) and longer degradation periods (~ 4 to 8 weeks) in vitro and...

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Functionalisation of a heat-derived and bio-inert albumin hydrogel with extracellular matrix by air plasma treatment

Ong

Zhao

Levy

et al. 2020

Sci Rep

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“…There are several studies showing that albumin is an efficient coating for bone-related biomaterials associated with increasing seeding efficiency, cell proliferation and calcium deposition in vitro [23][24][25], and bone remodelling in vivo [24][25][26][27][28]. However, the use of albumin hydrogels in bone tissue engineering remains under-utilised [29]. Fibrin is one of the most promising biopolymers used for bone and cardiovascular tissue engineering applications due to a combination of excellent biocompatibility, biodegradability, intrinsic bioactivity, and many other unique characteristics [30,31].…”

Section: Of 17mentioning

confidence: 99%

Albumin-Enriched Fibrin Hydrogel Embedded in Active Ferromagnetic Networks Improves Osteoblast Differentiation and Vascular Self-Organisation

Levy¹,

Ong²,

Birch³

et al. 2019

Preprint

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Porous coatings on prosthetic implants encourage implant fixation. Enhanced fixation may be achieved using a magneto-active porous coating that can deform elastically in vivo on application of an external magnetic field, straining in-growing bone. Such coating, made of 444 ferritic stainless steel fibres, was previously characterised in terms of its mechanical and cellular responses. In this work, co-cultures of human osteoblasts and endothelial cells were seeded into a novel fibrin-based hydrogel embedded in a 444 ferritic stainless steel fibre network. Albumin was successfully incorporated into fibrin hydrogels improving the specific permeability and the diffusion of fluorescently-tagged dextrans without affecting their Young’s modulus. The beneficial effect of albumin was demonstrated by upregulation of osteogenic and angiogenic gene expression. Furthermore, mineralisation, extracellular matrix production and formation of vessel-like structures were enhanced in albumin-enriched fibrin hydrogels compared to fibrin hydrogels. Collectively, the results indicate that the albumin-enriched fibrin hydrogel is a promising bio-matrix for bone tissue engineering and orthopaedic applications.

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Amyloid‐Based Albumin Hydrogels

Diaz

Missirlis

2022

Adv Healthcare Materials

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Amyloid fibrils may serve as building blocks for the preparation of novel hydrogel materials from abundant, low‐cost, and biocompatible polypeptides. This work presents the formation of physically cross‐linked, self‐healing hydrogels based on bovine serum albumin at room temperature through a straightforward disulfide reduction step induced by tris (2‐carboxyethyl) phosphine hydrochloride. The structure and surface charge of the amyloid‐like fibrils is determined by the pH of the solution during self‐assembly, giving rise to hydrogels with distinct physicochemical properties. The hydrogel surface can be readily functionalized with the extracellular matrix protein fibronectin and supports cell adhesion, spreading, and long‐term culture. This study offers a simple, versatile, and inexpensive method to prepare amyloid‐based albumin hydrogels with potential applications in the biomedical field.

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Albumin‐based hydrogels for regenerative engineering and cell transplantation

Cited by 75 publications

References 51 publications

Functionalisation of a heat-derived and bio-inert albumin hydrogel with extracellular matrix by air plasma treatment

Functionalisation of a heat-derived and bio-inert albumin hydrogel with extracellular matrix by air plasma treatment

Albumin-Enriched Fibrin Hydrogel Embedded in Active Ferromagnetic Networks Improves Osteoblast Differentiation and Vascular Self-Organisation

Amyloid‐Based Albumin Hydrogels

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