Albumin−CdTe Nanoparticle Bioconjugates:  Preparation, Structure, and Interunit Energy Transfer with Antenna Effect

Mamedova, Nataliya N.; Kotov, Nicholas A.; Rogach, Andrey L.; Studer, Joe

doi:10.1021/nl015519n

Cited by 418 publications

(334 citation statements)

References 43 publications

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“…Research in semiconducting nanoparticles is one of the most investigated subjects, due to their wide field of applications. Recently, the surface modification of fluorescent semiconductor nanoparticles by biomolecules and nucleic acids, has added a new dimension to nanoparticle research, with respect to their biological applications (Mamedova et al 2001). This article deals with the preparation of CdS nanoparticles using the wet chemical method.…”

Section: Introductionmentioning

confidence: 99%

Studies on the dielectric properties of CdS nanoparticles

Sagadevan

2013

Appl Nanosci

150

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CdS is one of the most important II-VI semiconductors with applications in solar cells, optoelectronics and electronic devices. CdS nanoparticles were synthesized by the wet chemical method. The crystal structure and grain size of the particles were determined by X-ray diffraction. The optical properties were studied by the ultraviolet-visible absorption spectrum. The dielectric properties of CdS nanoparticles were studied in the frequency range of 50 Hz-5 MHz at different temperatures. The frequency dependence of the dielectric constant and dielectric loss is found to decrease with an increase in the frequency at different temperatures. The dielectric properties of CdS nanoparticles are found to be significantly enhanced specially in the low frequency range due to confinement. Further, electronic properties, such as valence electron plasma energy, average energy gap or Penn gap, Fermi energy and electronic polarizability of the CdS nanoparticles were calculated. The AC electrical conductivity measurements reveal that the conduction depends on both the frequency and the temperatures.

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Section: Introductionmentioning

confidence: 99%

Studies on the dielectric properties of CdS nanoparticles

Sagadevan

2013

Appl Nanosci

150

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“…6,[19][20][21][22][23][24] During conjugation of bare CdTe nanoparticles to BSA, it was concluded that encapsulation was necessary to prevent particle growth, photoinduced decomposition, and conjugate aggregation. 13 Amorphous silica shells have been shown to provide some prime advantages necessary for bioconjugation of QDs. First, the silica shell prevents flocculation of particles, prevents species from adsorbing onto the surface, and helps to maintain the photoluminescence.…”

Section: Introductionmentioning

confidence: 99%

Silica-Coated CdTe Quantum Dots Functionalized with Thiols for Bioconjugation to IgG Proteins

et al. 2006

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Quantum dots (QDs) have been increasingly used in biolabeling recently as their advantages over molecular fluorophores have become clear. For bioapplications QDs must be water-soluble and buffer stable, making their synthesis challenging and time-consuming. A simple aqueous synthesis of silica-capped, highly fluorescent CdTe quantum dots has been developed. CdTe QDs are advantageous as the emission can be tuned to the near-infrared where tissue absorption is at a minimum, while the silica shell can prevent the leakage of toxic Cd 2+ and provide a surface for easy conjugation to biomolecules such as proteins. The presence of a silica shell of 2-5 nm in thickness has been confirmed by transmission electron microscopy and atomic force microscopy measurements. Photoluminescence studies show that the silica shell results in greatly increased photostability in Tris-borate-ethylenediaminetetraacetate and phosphate-buffered saline buffers. To further improve their biocompatibility, the silica-capped QDs have been functionalized with poly(ethylene glycol) and thiol-terminated biolinkers. Through the use of these linkers, antibody proteins were successfully conjugated as confirmed by agarose gel electrophoresis. Streptavidin-maleimide and biotinylated polystyrene microbeads confirmed the bioactivity and conjugation specificity of the thiolated QDs. These functionalized, silica-capped QDs are ideal labels, easily synthesized, robust, safe, and readily conjugated to biomolecules while maintaining bioactivity. They are potentially useful for a number of applications in biolabeling and imaging.

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“…In a typical synthesis [26][27], 5 mmol of CdCl 2 .2.5H 2 O was dissolved in 110 mL of water, and 12 mmol of TGA were added under stirring, followed by adjusting the pH to 11 by dropwise addition of 1 M NaOH solution. The mixed solution was placed in a three-necked flask deaerated by N 2 bubbling for 30 min.…”

Section: Preparation and Characterization Of Mqdsmentioning

confidence: 99%

Real time PCR based on Fluorescent Quenching of Mercaptoacetic Acid-Modified CdTe Quantum Dots for Ultrasensitive Specific Detection of Nucleic Acids

Cui¹,

Li²,

Huang³

et al. 2010

Nano BioMed ENG

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A ultra-sensitive, highly specific, real-time polymerase chain reaction system based on mercaptoacetic acid-modified CdTe nanocrystals(mQDs) is reported. With the addition of 3 nm mQDs into the PCR reagent, the photoluminescent(PL) intensities of mQDs decreased gradually as the DNA templates and PCR cycles increased, in an approximate negative linear relation to the DNA concentration logarithm or cycles, the PL peaks exhibited red-shifts synchronously. Mg 2+ ions decreased the PL intensity of mQDs in a dose-dependent means, and Taq DNA polymerase enhanced the PL intensity of mQDs in a dose-dependent means. Real-time PCR based on mQDs showed an increased sensitivity at least 103 fold higher than that based on SYBR Green I. The specificity of PCR was enhanced in the PCR reagent with less than 1.33mg/mL mQDs. The potential mechanism is also discussed. This novel PCR system based on mQDs has great potential in applications such as ultra-sensitive specific DNA or RNA detection, dynamic molecular imaging, and photoelectric biosensors.

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Albumin−CdTe Nanoparticle Bioconjugates: Preparation, Structure, and Interunit Energy Transfer with Antenna Effect

Cited by 418 publications

References 43 publications

Studies on the dielectric properties of CdS nanoparticles

Studies on the dielectric properties of CdS nanoparticles

Silica-Coated CdTe Quantum Dots Functionalized with Thiols for Bioconjugation to IgG Proteins

Real time PCR based on Fluorescent Quenching of Mercaptoacetic Acid-Modified CdTe Quantum Dots for Ultrasensitive Specific Detection of Nucleic Acids

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