Albumin from heated human plasma. I. Preparation and electrophoretic properties

An Economical Method for the Recovery of Albumin from Outdated Packed Cells using Polyethylene Glycol and Heat-Fractionation

“…The heat-caprylate fractionation method of Hoch and Chanutin [5] as modified by Schneider et al [7] of the German Red Cross Blood Transfusion Service, has sim plified the isolation of albumin from plasma. Wye and Kim [10] point out that the Ca nadian Red Cross Blood Transfusion Ser vice routinely uses a heat-caprylate fraction ation method to improve their yields and render pyrogenic albumin pyrogen free.…”

“…The NBTS routinely produces albumin of >99% purity from plasma by pooling Cohn fractions IV and V and heat-fractionating by a modified method of Hoch and Chanutin [5] using sodium caprylate and Nacetyl tryptophan as stabilizers [Dr. V. de Villiers, personal commun.]. An 11 kg batch of pooled crude albumin paste prepared as described above from packed cells, was fractionated by this meth od.…”

Catabolic Half-Lives and Antigenic Relationships of Native, Altered and Commercially Prepared Human Albumins in Rabbits

“…Thus, the differences in t*/2S duced from heated plasma by the procedure of Hoch and Chanutin [9]. Schneider et al…”

Precipitation Methods in Plasma Fractionation

“…Several years ago Chanutin and co-workers [14,31] observed the precipitation of plasma proteins by short-chain (C6-C12) fatty acids at pH 4.2. They showed that there were differences in the precipitating capacities of the different organic acids and that, as far as caprylic acid was concerned, y-globulin was precipi tated less than the a-or ^-globulins [14], Our studies have been limited to caprylic acid as this liquid can be easily handled and its use for many years as a stabilizer of intravenously-injected albumin has demonstrated its safety.…”