Albumin-hyaluronic acid colloidal nanocarriers: Effect of human and bovine serum albumin for intestinal ibuprofen release enhancement

Kovács, Alexandra N.; Katona, Gábor; Juhász, Ádám; Balogh, György Tibor; Csapó, Edit

doi:10.1016/j.molliq.2022.118614

Cited by 9 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The surface charge of polymer-modified lipids can be crucial for the encapsulation of the active ingredient, so we investigated this characteristic in this type of drug carriers. A PCD-04 particle charge detector (Mütek Analytic GmbH, Herrsching, Germany) was applied to determine quantitatively the different charge neutralization points of the liposome-based colloids [ 41 , 42 , 43 ]. The specific surface charge of the WSLP was determined using an anionic surfactant (SDS).…”

Section: Methodsmentioning

confidence: 99%

“…The effective permeability ( P e ), membrane retention (MR), and flux (Flux) of the samples were calculated using Equations (1)–(3):

where P e is the effective permeability coefficient (cm/s), A is the filter area (0.24 cm 2 ), V D and V A are the volumes in the donor (0.15 cm 3 ) and acceptor phase (0.3 cm 3 ), t is the incubation time (s), τ SS is the time to reach the steady state (s), C A ( t ) is the concentration of the compound in the acceptor phase at time point t (mol/cm 3 ), c D ( t ) is the concentration of the compound in the donor phase at time point t (mol/cm 3 ), c D (0) is the concentration of the compound in the donor phase at time point zero (mol/cm 3 ), and S is the solubility of KYNA in the donor phase determined by previously presented method [ 41 ].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Lipid-Based Nanocarriers for Delivery of Neuroprotective Kynurenic Acid: Preparation, Characterization, and BBB Transport

Juhász,

Ungor,

Varga

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Encapsulation possibilities of an extensively investigated neuroprotective drug (kynurenic acid, KYNA) are studied via lipid-based nanocarriers to increase the blood–brain barrier (BBB) specific permeability. The outcomes of various preparation conditions such as stirring and sonication time, concentration of the lipid carriers and the drug, and the drug-to-lipid ratio are examined. Considering the experimentally determined encapsulation efficiency, hydrodynamic diameter, and ζ-potential values, the initial lipid and drug concentration as well as the stirring and sonication time of the preparation were optimized. The average hydrodynamic diameter of the prepared asolectin-(LIP) and water-soluble lipopolymer (WSLP)-based liposomes was found to be ca. 25 and 60 nm under physiological conditions. The physicochemical characterization of the colloidal carriers proves that the preparation of the drug-loaded liposomes was a successful process, and secondary interactions were indicated between the drug molecule and the polymer residues around the WSLP membrane. Dissolution profiles of the active molecule under physiological conditions were registered, and the release of the unformulated and encapsulated drug is very similar. In addition to this outcome, the in vitro polar brain lipid extract (porcine)-based permeability test proved the achievement of two- or fourfold higher BBB specific penetration and lipid membrane retention for KYNA in the liposomal carriers relative to the unformatted drug.

show abstract

Section: Methodsmentioning

confidence: 99%

“…The effective permeability ( P e ), membrane retention (MR), and flux (Flux) of the samples were calculated using Equations (1)–(3):

Section: Methodsmentioning

confidence: 99%

Lipid-Based Nanocarriers for Delivery of Neuroprotective Kynurenic Acid: Preparation, Characterization, and BBB Transport

Juhász,

Ungor,

Varga

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…In 1934, Meyer et al from Columbia University in the United States successfully isolated hyaluronic acid from the vitreous body of the bull's eye [6], and since then, its extraction from the eyes of animals, specifically from vitreous and cartilage tissues, has been established. Alexandra et al demonstrated the effective suitability of serum albumin-hyaluronic acid conjugates as potential colloidal particles for administration [7]. As a significant participant in human physiological processes, the level of hyaluronic acid in serum can reflect the function and damage of epidermal cells in the liver [8].…”

Section: Introductionmentioning

confidence: 99%

Microfluidic technology combined with density functional theory for investigating terahertz spectral characteristics of hyaluronic acid and its constituent monomers

Yan,

Zhao,

Hou

et al. 2024

Phys. Scr.

View full text Add to dashboard Cite

Terahertz spectroscopy offers a valuable approach for material research, particularly in identifying unique characteristics of biological macromolecules within the terahertz band. Hyaluronic acid, a polysaccharide involved in vital life processes within the human body, holds significant application potential in medicine and healthcare. In this study, we employed a terahertz time-domain spectroscopy system combined with microfluidic chip to investigate the terahertz absorption spectra of hyaluronic acid and its constituent monomers, namely D-glucuronic acid and N-acetylglucosamine. The analysis covered solid state under vacuum and humidity of 19.8% environment, as well as solution state, spanning a frequency range of 0.5~2.5 THz. In addition, using CASTEP software package and PBE density functional, the molecular structures of D-glucuronic acid and N-acetylglucosamine were simulated using periodic boundary conditions, and the vibrational modes of absorption peaks were assigned to determine their sources. The results indicate that terahertz spectroscopy is highly sensitive to the structure and environment of substances, and the absorption peaks of materials in a vacuum are primarily attributed to interactions both between molecules and within molecules.

show abstract

“…These neutralization methods are mainly driven by ionic interactions, and hydrophobic interactions presumably have importance as well [17], but the knowledge of these processes is still incomplete. Numerous HyA-macromolecule complexes have already been formed and studied [18], among which the most common ones are HyA-polymer (chitosan [19][20][21] and PEI [22]) and HyA-protein (zein [23], BSA [24], and HSA [16]) interactions. The obtained complexes are suitable for the delivery of small molecules, but the targeting of nucleic acids is also possible with them [25,26].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Concentration, Temperature, and pH on the Formation of Hyaluronic Acid–Surfactant Nanohydrogels

Serés

Csapó

Varga

et al. 2023

Gels

Self Cite

View full text Add to dashboard Cite

The assembly of colloidal hyaluronic acid (HyA, as a polysaccharide) based hydrogel particles in an aqueous medium is characterized in the present paper, with an emphasis on the particular case of nanohydrogels formed by surfactant-neutralized polysaccharide networks. The structural changes and particle formation process of polysaccharide- and cationic-surfactant-containing systems were induced by the charge neutralization ability and the hydrophobic interactions of cetyltrimethylammonium bromide (CTAB) under different conditions. Based on the rheological, light scattering, ζ-potential, turbidity, and charge titration measurements, it can be concluded that the preparation of the HyA-CTAB particles can be greatly controlled. The results indicate that more available negative charges can be detected on the polymer chain at smaller initial amounts of HyA (cHyA < 0.10 mg/mL), where a molecular solution can be formed. The change in the pH has a negligible effect on the formation process (particle aggregation appears at nCTAB/nHyA,monomer~1.0 in every case), while the temperature dependence of the critical micelle concentration (c.m.c.) of CTAB determines the complete neutralization of the forming nanohydrogels. The results of our measurements confirm that after the appearance of stable colloidal particles, a structural change and aggregation of the polymer particles take place, and finally the complete charge neutralization of the system occurs.

show abstract

Albumin-hyaluronic acid colloidal nanocarriers: Effect of human and bovine serum albumin for intestinal ibuprofen release enhancement

Cited by 9 publications

References 34 publications

Lipid-Based Nanocarriers for Delivery of Neuroprotective Kynurenic Acid: Preparation, Characterization, and BBB Transport

Lipid-Based Nanocarriers for Delivery of Neuroprotective Kynurenic Acid: Preparation, Characterization, and BBB Transport

Microfluidic technology combined with density functional theory for investigating terahertz spectral characteristics of hyaluronic acid and its constituent monomers

The Effect of Concentration, Temperature, and pH on the Formation of Hyaluronic Acid–Surfactant Nanohydrogels

Contact Info

Product

Resources

About