Alcaligenes latus, a New Species of Hydrogen-Utilizing Bacteria

Palleroni, Norberto J.; Palleroni, Alicia V.

doi:10.1099/00207713-28-3-416

Cited by 77 publications

(31 citation statements)

References 8 publications

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“…6, an initial pH value of 6.5 gave the best results; pH values that differed even slightly from the optimum reduced the culture performance. These results are consistent with Palleroni and Palleroni [14],who recommended a pH range of 6.0 -7.5 for microbial growth. The initial pH value may have affected the bioavailability of some of the trace elements.…”

Section: Influence Of Initial Ph and Temperaturesupporting

confidence: 81%

“…The cells occur singly, in pairs, or in short chains [14,15]. The bacterium is sluggishly motile by means of 5-10 peritrichous flagella [14,15]. Optimal growth temperature is about 35°C.…”

Section: Introductionmentioning

confidence: 99%

“…The bacterial cells are short rods or coccoids, 1.2-2.4 m in diameter and 1.6 -2.4 m in length. The cells occur singly, in pairs, or in short chains [14,15]. The bacterium is sluggishly motile by means of 5-10 peritrichous flagella [14,15].…”

Section: Introductionmentioning

confidence: 99%

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Fermentation optimization for the production of poly(β-hydroxybutyric acid) microbial thermoplastic

Grothe

Moo‐Young

Chisti

1999

Enzyme and Microbial Technology

195

114

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Batch culture of Alcaligenes latus, American Type Culture Collection 29713, was investigated for producing the intracellular bioplastic poly(␤-hydroxybutyric acid) (PHB). A central, composite experimental design was used to optimize the composition of the culture medium for maximizing the productivity of PHB. Investigated were the effects of temperature, the initial culture pH, the ionic strength of the medium, the concentration of trace elements, the type of nitrogen source, and the carbon-to-nitrogen ratio. The optimal temperature for growth and PHB synthesis appeared to be 33°C; however, over the 25-37°C range, the effect of temperature was negligible. An initial pH value of 6.5 gave the best results; pH values that differed even slightly from the optimum reduced the culture performance. Typical culture characteristics were: 0.075/h maximum specific growth rate, 0.38 g/l h maximum specific sucrose consumption rate, and 0.15 g/l h maximum specific PHB production rate. PHB was lost because of hydrolysis in the stationary phase, suggesting critical importance of timing the harvest. Under the best conditions, PHB constituted up to 63% of dry cell mass after 93 h of culture. The average biomass yield coefficient on sucrose was about 0.4 kg/kg. Of the four nitrogen sources-ammonium chloride, ammonium sulfate, ammonium nitrate, and urea-used, only the first two supported the culture satisfactorily. The biomass and PHB showed clear yield maxima at 1.5 g/l ammonium chloride (C:N ratio ϭ 21.5) and 1.4 g/l ammonium sulfate (C:N ratio ϭ 28.3). The yields were higher with ammonium sulfate and were relatively more sensitive to changes in its concentration. Ionic strength had a strong negative effect on PHB productivity. The highest PHB yield occurred at 4 g/l phosphate buffer concentration. Iron appeared to have the potential to enhance the proportion of PHB in the cells.

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Section: Influence Of Initial Ph and Temperaturesupporting

confidence: 81%

“…The cells occur singly, in pairs, or in short chains [14,15]. The bacterium is sluggishly motile by means of 5-10 peritrichous flagella [14,15]. Optimal growth temperature is about 35°C.…”

Section: Introductionmentioning

confidence: 99%

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Fermentation optimization for the production of poly(β-hydroxybutyric acid) microbial thermoplastic

Grothe

Moo‐Young

Chisti

1999

Enzyme and Microbial Technology

195

114

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“…The presence of photosynthetic pigments, described for members of Rubrivivax gelatinosus (Willems et al, 1991), constitutes another distinction between isolate N2-680 T and this species. Members of Alcaligenes latus can grow autotrophically in the presence of hydrogen gas, are able to reduce nitrate and can fix nitrogen (Palleroni & Palleroni, 1978;Busse & Auling, 1992). All these characteristics were absent for isolate N2-680 T .…”

Section: Resultsmentioning

confidence: 99%

“…Data were obtained from Busse & Auling (1992), Kalmbach et al (1999), Malmqvist et al (1994), Moreira et al (2000), Palleroni & Palleroni (1978), Spring et al (1996), Takeda et al (2002) and Willems et al (1991) and Aquabacterium and the species Alcaligenes latus, is the temperature range of growth. Strain N2-680 T differs from species of the genus Leptothrix in the absence of a cell sheath and the inability to produce manganese oxides, as is typical for these organisms (Siering & Ghiorse, 1996).…”

Section: Resultsmentioning

confidence: 99%

Caenibacterium thermophilum gen. nov., sp. nov., isolated from a thermophilic aerobic digester of municipal sludge

Manaia

Nunes

Nogales

2003

International Journal of Systematic and Evolutionary Microbiology

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A bacterial strain, N2-680 T (=DSM 15264 T =LMG 21760 T ), isolated from a thermophilic aerobic digester of municipal sludge, was characterized with respect to its morphology, physiology and taxonomy. Phenotypically, the isolate was a Gram-negative rod with a polar flagellum, catalase-and oxidase-positive, containing cytoplasmic inclusions of poly-b-hydroxybutyrate and had an optimal growth temperature of about 47 6C. Strain N2-680 T was unable to reduce nitrate and could use organic acids, amino acids and carbohydrates as single carbon sources. Chemotaxonomic analysis revealed that ubiquinone 8 was the major respiratory quinone of this organism and that phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids. At 50 6C, the major components in fatty acid methyl ester analysis were C 16 : 0 and cyclo-C 17 : 0 . The highest 16S rDNA sequence identity of isolate N2-680 T was to Leptothrix mobilis and Ideonella dechloratans (95?7 %) and to Rubrivivax gelatinosus and Aquabacterium commune (95?6 %). 16S rDNA sequence similarities to species of two related thermophilic genera, Caldimonas manganoxidans and Tepidimonas ignava, were lower (93?6 and 94?7 %). On the basis of phylogenetic analyses and physiological and chemotaxonomic characteristics, it is proposed that isolate N2-680 T represents a new genus and species, for which the name Caenibacterium thermophilum gen. nov., sp. nov. is proposed.

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Alcaligenaceae

Busse¹,

Auling²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Alcaligenes latus, a New Species of Hydrogen-Utilizing Bacteria

Cited by 77 publications

References 8 publications

Fermentation optimization for the production of poly(β-hydroxybutyric acid) microbial thermoplastic

Fermentation optimization for the production of poly(β-hydroxybutyric acid) microbial thermoplastic

Caenibacterium thermophilum gen. nov., sp. nov., isolated from a thermophilic aerobic digester of municipal sludge

Alcaligenaceae

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