Alcaloïdes des Annonacées, 77. Alcaloïdes de Duguetia spixiana

Debourges, D.; Roblot, François; Hocquemiller, Reynald; Cavé, André

doi:10.1021/np50052a015

Cited by 19 publications

(13 citation statements)

References 13 publications

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“…The presence of the alporphine alkaloids in the Annonaceae family is constant in species of the genus Annona with reports of the isolation and identification in leaves. [18] In this way, the following compounds were found in the chemical identification of the A. coriacea Mart. hydroalcoholic extract [Table 2].…”

Section: Resultsmentioning

confidence: 99%

Chemical characterization and cytoprotective effect of the hydroethanol extract from Annona coriacea Mart. (Araticum)

Júnior¹,

Coutinho²,

Boris³

et al. 2016

Phcog Res

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Introduction:Annona coriacea Mart. (araticum) is a widely distributed tree in the cerrado. Its value is attributed principally to the consumption of its fruit which possesses a large nutritive potential. The objective was to identify the chemical profile and evaluate the antimicrobial and cytoprotective activity of the hydroethanol extract of A. coriacea Mart. (HEAC) leaves against the toxicity of mercury chloride.Materials and Methods:The characterization of components was carried out using high-performance liquid chromatography (HPLC). The minimum inhibitory concentration (MIC) was determined by microdilution method in broth with strains of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. For evaluation of the modulatory and cytoprotective activity of aminoglycoside antibiotics (gentamicin and amikacin) and mercury chloride (HgCl2), the substances were associated with the HEAC at subinhibitory concentrations (MIC/8).Results and Discussion:The HPLC analysis revealed the presence of flavonoids such as Luteolin (1.84%) and Quercetin (1.19%) in elevated concentrations. The HEAC presented an MIC ≥512 μg/mL and significant antagonistic action in aminoglycosides modulation, and it also showed cytoprotective activity to S. aureus (significance P < 0.0001) and E. coli (significance P < 0.05) bacteria against the mercury chloride heavy metal with significance, this action being attributed to the chelating properties of the flavonoids found in the chemical identification.Conclusions:The results acquired in this study show that the HEAC presents cytoprotective activity over the tested strains in vitro and can also present antagonistic effect when associated with aminoglycosides, reinforcing the necessity of taking caution when combining natural and pharmaceutical products.SUMMARY The hydroalcoholic extract of A. coriacea Mart. presents in vitro cytoprotective activity against the toxic effect of Hg. Abbreviations Used: HPLC-DAD: High-performance liquid chromatography with a diode array detector; MIC: Minimum inhibitory concentration; DMSO: Dimethyl sulfoxide

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Section: Resultsmentioning

confidence: 99%

Chemical characterization and cytoprotective effect of the hydroethanol extract from Annona coriacea Mart. (Araticum)

Júnior¹,

Coutinho²,

Boris³

et al. 2016

Phcog Res

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“…Uma informação importante que pode ser obtida no espectro de RMN 1 H para esse tipo de alcalóide 7-oxigenado é a definição da estereoquímica cis ou trans dos hidrogênios H-6a e H-7. Na espécie Duguetia spixiana , , dentre outras espécies da família Annonaceae 5 , os alcalóides 7-oxigenados apresentam configuração trans e o sinal do espectro é constituído de dupletos com constantes de acoplamento de 9 a 12 Hz (mais comum, 12 Hz) 11,12 . Na estereoquímica cis, o J observado foi de 2 a 3 Hz, como descrito para alcalóides isolados do gênero Stephania (Menispermaceae) 5 .…”

Section: Resultsunclassified

Estudo fitoquímico das cascas do caule de Duguetia glabriuscula - Annonaceae, biomonitorado pelo ensaio de toxicidade frente a Artemia salina leach

Siqueira¹,

Ziminiani²,

Resende³

et al. 2001

Quím. Nova

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“…Estudos anteriores apresentaram rendimentos de alcalóides totais para caule de Duguetia spixiana (Annonaceae) em 0,3 % e 0,2 % (DEBOURGES et al,1987;, contudo para Annona foi encontrado dados de folha com rendimento de 0,5 % de alcalóides totais (FISCHER et al, 2004 ocorrer subseqüentes perdas de radicais metilas ou metoxilas para a formação de um fragmento com número par de elétrons (OHASHI et al,1963).…”

Section: Extração De Alcalóides Totais (At)unclassified

Avaliação da atividade antibacteriana e citotóxica dos alcalóides isoquinolínicos de Annona hypoglauca Mart

Rinaldi¹

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Annona hypoglauca Mart. was collected in the flooded areas of the Amazonian Forest near Manaus (Brazil). The alkaloids were obtained from the stems crude extract by acid-base partitioning and the remaining alkaloid-free extract was partitioned with organic solvents of different polarity. The GC/MS analysis of the total alkaloids allowed the identification of seven aporphine alkaloids (actinophanine, anonaine, glaucine, isoboldine, isodomesticine, nornuciferine and roemerine) and possibly two proberberine alkaloids (scoulerine and caseadine). The total alkaloids were fractionated by column chromatography and further purified by preparative thin-layer chromatography allowing the isolation of two aporphine alkaloids: actinodaphnine and isoboldine. The structures of these natural products were defined based on their spectral data, including 1 H NMR, 13 C NMR,

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Alcaloïdes des Annonacées, 77. Alcaloïdes de Duguetia spixiana

Cited by 19 publications

References 13 publications

Chemical characterization and cytoprotective effect of the hydroethanol extract from Annona coriacea Mart. (Araticum)

Chemical characterization and cytoprotective effect of the hydroethanol extract from Annona coriacea Mart. (Araticum)

Estudo fitoquímico das cascas do caule de Duguetia glabriuscula - Annonaceae, biomonitorado pelo ensaio de toxicidade frente a Artemia salina leach

Avaliação da atividade antibacteriana e citotóxica dos alcalóides isoquinolínicos de Annona hypoglauca Mart

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