Alcohol dehydrogenase isoenzymes in chickpea cotyledons

Gomes, Joseph; S, Jadrić; Winterhalter, Michael; Brkić, Snježana

doi:10.1016/0031-9422(82)80114-3

Cited by 15 publications

(8 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This could be due to the use of a gel system with a pH different from that used by Malvolti and Fineschi (1987) for European chestnut. Gomes et al (1982) indicated that ADH-2 and ADH-3 activity could be greatly inhibited at pH 6.…”

Section: Methodsmentioning

confidence: 99%

Genetic Analysis of 11 Polymorphic Isozyme Loci in Chestnut Species and Characterization of Chestnut Cultivars by Multi-locus Allozyme Genotypes

Huang¹,

Dane²,

Norton³

1994

jashs

View full text Add to dashboard Cite

Allozyme polymorphism in chestnut (Castanea) species was investigated using isoelectric focusing in thin-layer polyacrylamide slab gels. Genetic analysis of the progenies of intraspecific crosses and interspecific F 2 s and backcrosses (BC 1 s) allowed the verification of 11 polymorphic isozyme loci from 11 enzyme systems. The following loci were defined: Acp, Adh, Est-1, Est-2, Est-5, Me, Prx-1, Prx-2, Prx-3, Skd-3, and Skd-4. All polymorphic loci behaved as single-locus Mendelian genes. Skd showed unique species specificity. Skd-1 and Skd-2 were unique to the American chestnut (C. dentata Borkh.) and the European chestnut (C. sativa Mill.), whereas Skd-3 and Skd-4 were unique to the Chinese chestnut (C. mollissima Bl.) and the Japanese chestnut (C. crenata Sieb.). Linkage analysis revealed linkage for three pairs of loci: Skd-3/Skd-4, Est-1/Est-2, and Est-5/Prx-1. The single-tree progeny method was used successfully for isozyme genetic analysis. Forty-seven chestnut cultivars in six chestnut species were characterized using 12 isozyme loci and can be unambiguously identified by 12 multi-locus genotypes. The interspecific and geographic relationships among species were also discussed.

show abstract

Section: Methodsmentioning

confidence: 99%

Genetic Analysis of 11 Polymorphic Isozyme Loci in Chestnut Species and Characterization of Chestnut Cultivars by Multi-locus Allozyme Genotypes

Huang¹,

Dane²,

Norton³

1994

jashs

View full text Add to dashboard Cite

show abstract

“…Similar to lipoxygenase, alcohol dehydrogenase also has isozymes. Gomes, Jadrić, Winterhalter and Brkić [30] have reported three isozymes in chickpea cotyledon. This could suggest that chickpea and fava bean contain alcohol dehydrogenase which converts aldehydes to alcohols.…”

Section: Presence Of Acid In Chickpea and Fava Bean Suggests Alcohol mentioning

confidence: 99%

“…This is not surprising, as legumes contain alcohol dehydrogenases that act on products of the lipoxygenase pathway described above. For example, three isozymes of alcohol dehydrogenase have been described in chickpea, which catalyse the interconversion of aldehydes, alcohol and acid [30], possibly explaining the abundance of 1-hexanol and 1-pentanol in chickpea. Other alcohols prominent in legumes possibly arising from enzymatic actions include 1-penten-3-ol and 1-octen-3-ol, two compounds which had been described as having undesirable odour.…”

Section: Aldehydes Alcohols Ketones and Terpenes In Studied Legumesmentioning

confidence: 99%

A Comprehensive Characterisation of Volatile and Fatty Acid Profiles of Legume Seeds

Khrisanapant

Kebede

Leong

et al. 2019

Foods

View full text Add to dashboard Cite

Legumes are rich in unsaturated fatty acids, which make them susceptible to (non) enzymatic oxidations leading to undesirable odour formation. This study aimed to characterise the volatile and fatty acid profiles of eleven types of legumes using headspace solid-phase microextraction gas chromatography–mass spectrometry (HS-SPME-GC-MS) and GC coupled with a flame ionisation detector (GC-FID), respectively. Volatile aldehydes, alcohols, ketones, esters, terpenes and hydrocarbons were the chemical groups identified across all the legumes. The lipids comprised palmitic, stearic, oleic, linoleic and α-linolenic acids, with unsaturated fatty acids comprising at least 66.1% to 85.3% of the total lipids for the legumes studied. Multivariate data analysis was used to compare volatile and fatty acid profiles between legumes, which allow discriminant compounds pertinent to specific legumes to be identified. Results showed that soybean, chickpea and lentil had distinct volatile and fatty acid profiles, with discriminating volatiles including lactone, ester and ketone, respectively. While all three Phaseolus cultivars shared similar volatile profiles, 3-methyl-1-butanol was found to be the only volatile differentiating them against the other eight legumes. Overall, this is the first time a multivariate data analysis has been used to characterise the volatile and fatty acid profiles across different legume seeds, while also identifying discriminating compounds specific for certain legume species. Such information can contribute to the creation of legume-based ingredients with specific volatile characteristics while reducing undesirable odours, or potentially inform relevant breeding programs.

show abstract

“…Interestingly, both of these pathways require rapid methanol oxidation. Yet most alcohol dehydrogenases in plants catalyze negligible levels of methanol oxidation (Erikkson, 1968;Gomes et al, 1982;Grondal et al, 1983;Leblova and Perglerova, 1976;Swaisgood and Pattee, 1968); hence, very little is known about the occurrence of methanol oxidative enzymes in plant tissue. Understanding the pathways of methanol assimilation and detoxification in plants will ultimately require the characterization of the pathway of methanol oxidation in photosynthesizing plant tissue.…”

Section: Irreproducibility Of Reports Of Methanol As a Growth Promotermentioning

confidence: 99%

The Role of Methanol in Promoting Plant Growth: A Current Evaluation

McGiffen¹,

Manthey²

1996

HortSci

View full text Add to dashboard Cite

property does not constitute a guarantee or warranty of the product by the U.S. Dept. of Agriculture or the Univ. of California and does not imply its approval to the exclusion of other products that also may be suitable. The cost of publishing this paper was defrayed in part by the payment of page charges. Under postal regulations, this paper therefore must be hereby marked advertisement solely to indicate this fact. 1 To whom reprint requests should be addressed.

show abstract

Alcohol dehydrogenase isoenzymes in chickpea cotyledons

Cited by 15 publications

References 23 publications

Genetic Analysis of 11 Polymorphic Isozyme Loci in Chestnut Species and Characterization of Chestnut Cultivars by Multi-locus Allozyme Genotypes

Genetic Analysis of 11 Polymorphic Isozyme Loci in Chestnut Species and Characterization of Chestnut Cultivars by Multi-locus Allozyme Genotypes

A Comprehensive Characterisation of Volatile and Fatty Acid Profiles of Legume Seeds

The Role of Methanol in Promoting Plant Growth: A Current Evaluation

Contact Info

Product

Resources

About