Genetic Analysis of 11 Polymorphic Isozyme Loci in Chestnut Species and Characterization of Chestnut Cultivars by Multi-locus Allozyme Genotypes

Huang, Hongwen; Dane, Fenny; Norton, J. D.

doi:10.21273/jashs.119.4.840

Cited by 31 publications

(15 citation statements)

References 38 publications

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“…Artificial interspecific crosses among chestnuts have been used to introgress disease and/or pest resistance traits in chestnut breeding programs (e.g., Clapper, 1954;Diller and Clapper, 1969). Molecular markers such as allozymes also have been applied in chestnut breeding (Huang et al, 1994). Therefore, these highly polymorphic SSR loci would be more favorable to breeding programs in genus Castanea.…”

Section: Resultsmentioning

confidence: 99%

Microsatellite Loci Transferability in Chestnut

Wang¹,

Kang²,

Huang³

2008

J. Amer. Soc. Hort. Sci.

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Cross-species amplification of 55 microsatellite loci developed in european chestnut (Castanea sativa Mill.) and japanese chestnut (C. crenata Sieb & Zucc.) was tested in three chestnut species from China [C. mollissima Blume, C. seguinii Dode, and C. henryi (Skan.) Rehder & Wilson]. Among all the tested loci, 47 (85.5%), 47 (85.5%), and 44 (80%) were successfully amplified in each of the three Chinese species, respectively. All microsatellite loci tested from C. crenata successfully amplified in the Chinese species, while only 80.5%, 80.5%, and 73.2% of the loci originating from C. sativa amplified in the three Chinese species. The level of polymorphism and mean number of alleles was 58.2% and 4.12 for C. mollissima, 60% and 4.64 for C. seguinii, and 60% and 4.76 for C. henryi, with mean observed heterozygosity ranging from 0.440 to 0.549 and mean expected heterozygosity ranging from 0.506 to 0.615. Transferability of Castanea Mill. microsatellites provides a powerful tool for chestnut breeding programs and conservation genetic studies of Castanea species.

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Section: Resultsmentioning

confidence: 99%

Microsatellite Loci Transferability in Chestnut

Wang¹,

Kang²,

Huang³

2008

J. Amer. Soc. Hort. Sci.

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“…For the characterization of genetic resources within Castanea sp. several methods have been used: morphological or phenological studies, isozyme markers, or random amplified polymorphic DNA (RAPD) markers (Bassi and Marangoni 1984, Huang et al 1994, Gao et al 2001. Simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) analyses have been introduced and widely applied to characterise genetic resources of many plant species (Vos et al 1995, Mukherjee et al 2003 including genus Castanea (Yamamoto et al 1998, Botta et al 1999.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 99%

Characterisation of Chinese elite cultivars and genetic resources of chestnut by AFLP

Ovesná¹,

Kučera²,

Jiang³

et al. 2005

Biologia plant.

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We selected a informative set of twelve amplified fragment length polymorphism (AFLP) primer pairs suitable for evaluation of Chinese chestnut (Castanea mollissima Blume) genotypes. Cluster analysis based on 198 polymorphic AFLP amplified by these 12 primer pairs clearly divided investigated genotypes according to their place of origin. We showed, that genetic basis of modern genotypes is narrow also in the case of this species.

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“…The most frequently employed biochemical markers are isoenzymes Fineschi 1987, 1988;Villani et al 1987Villani et al , 1991Villani et al , 1992Giannini et al 1993;Pereira and Fernandez 1993;Huang et al 1994;Pereira Lorenzo et al 1996b; Podjavor s sek et al…”

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confidence: 99%

Phenotypic and Genotypic Diversity of European Chestnut (Castanea sativa Mill.) in Slovenia – Opportunity for Genetic Improvement

Solar

Podjavoršek²,

Štampar

2005

Genet Resour Crop Evol

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Phenotypic diversity of 244 chestnut trees was investigated. They originate from the Mediterranean and from two continental regions in Slovenia. In 3 years of analyses, length, diameter, thickness and weight of fruits; length and width of hilum, the pellicle intrusion; shape and colour of fruits; and the embryony were dealt with. The continental trees have smaller fruits than the Mediterranean trees; their fruits show greater variability in shape and the pellicle intrusion is stronger. They also exhibit polyembriony more frequently and they rarely have darker stripes. The sample of 46 trees from all three regions was used for the comparison between the phenotypic and genotypic diversity which had been investigated by RAPD analysis. In both cases, the UPGMA method was used for the classification of the trees into groups. Six pomological clusters were established. The clusters I, II, III and V comprise only the trees from the continental part. The cluster IV comprises nine trees of the marron type from the Mediterranean and one KOZ1 tree with equal pomological characteristics but originating from the continental part. The cluster VI is composed of eight trees from the continental part and one RAV3 tree whose fruits are very small and it originates from the Mediterranean. The Jaccard's coefficient of similarity is used in order to evaluate genetic relations. On the RAPD dendrogram four clusters of trees are defined. The continental trees are divided into three clusters and exhibit greater genotypic diversity than the Mediterranean trees which form only one cluster. With the RAPD analyses the differentiation of the trees with regard to their pomological traits is determined in 60-90% of the cases. The outcome of the research point to the fact that the Slovene chestnut is a rich source of genetic diversity and is very suitable for further breeding purposes.

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Genetic Analysis of 11 Polymorphic Isozyme Loci in Chestnut Species and Characterization of Chestnut Cultivars by Multi-locus Allozyme Genotypes

Cited by 31 publications

References 38 publications

Microsatellite Loci Transferability in Chestnut

Microsatellite Loci Transferability in Chestnut

Characterisation of Chinese elite cultivars and genetic resources of chestnut by AFLP

Phenotypic and Genotypic Diversity of European Chestnut (Castanea sativa Mill.) in Slovenia – Opportunity for Genetic Improvement

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