ALDH isozymes downregulation affects cell growth, cell motility and gene expression in lung cancer cells

Moreb, Jan S.; Baker, Henry V.; Chang, Lung‐Ji; Amaya, Maria Jimena; López, María Cecilia; Ostmark, Blanca; Chou, Wayne

doi:10.1186/1476-4598-7-87

Cited by 140 publications

(124 citation statements)

References 64 publications

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“…Moreb et al 35 recently reported that the knock-down of ALDH1A1 and ALDH3A1 resulted in decreased proliferative activity of lung adenocarcinoma cell lines. Since the level of ALDH did not affect the proliferative activity of Hodgkin lymphoma cell lines in the present study, the effect of ALDH may differ among cancer cell types.…”

Section: Relationship Between Ros-low and Aldh-high Populationsmentioning

confidence: 99%

Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells

Ikeda

Mamat

Tian

et al. 2012

Laboratory Investigation

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Tumor cells with tumorigenic potential might be limited to a small population of cells, called cancer-initiating cells (CICs). CICs efficiently form colonies in vitro, yield both CIC and non-CIC populations, maintain reactive oxygen species (ROS) at low levels, show high aldehyde dehydrogenase (ALDH) activity, and are mostly in a quiescent state of the cell cycle. CICs of Hodgkin lymphoma (HL) are small in size, with low levels of ROS. The relationship between ROS level and ALDH activity in CICs was examined in HL cell lines. ROS-low and ALDH-high populations formed colonies in semi-solid cultures more efficiently than ROS-high and ALDH-low populations. ALDH-high populations yielded both ALDH-low and -high populations, whereas ALDH-low populations rarely yielded an ALDH-high population. The number of cells in a quiescent state was significantly greater in ROS-low than in ROS-high cells, whereas that of ALDH-high and ALDH-low cells was comparable to each other. These findings show that ALDH-high and ROS-low cells share CIC-like potential, but they differ in their cell cycle status, suggesting that CICs are comprised of cells with heterogeneous characteristics. . CICs are present in several tumors, such as leukemia, breast, brain, and colon cancers. 1-8 CICs yield both CICs and non-CICs, whereas non-CICs rarely yield CICs. 1,5 CICs efficiently efflux anti-tumor chemicals, form colonies in semi-solid culture, and degrade reactive oxygen species (ROS) that are generated upon radiation-induced apoptosis. [1][2][3][4][5][6][7][8][9] Recent studies have pointed to high aldehyde dehydrogenase (ALDH) activity as a candidate CIC characteristic. [10][11][12][13][14][15][16][17][18][19][20] ALDH oxidizes retinol to retinoic acid in the early stages of stem cell differentiation. 11 High ALDH activity is detected in both physiological stem cells, such as hematopoietic and neural stem cells, 21,22 and CICs of human multiple myeloma, acute myeloid leukemia, and cancers of the lung and breast, 10,[12][13][14][15][16][17] indicating that ALDH activity may be a common marker for both normal and malignant stem cell populations. To date, information on ALDH activity in CICs of malignant lymphomas is extremely limited.The cellular origin of Hodgkin lymphoma (HL) had been controversial; however, recent molecular biology studies have revealed that HL is mostly a neoplasia of B lymphocytes. 23,24 HL can be diagnosed by the presence of multinucleated ReedSternberg (RS) cells and mononuclear Hodgkin cells intermingled with small lymphocytes and various inflammatory cells. They are regarded as pathognomonic for HL and share the expression of some cell markers with Hodgkin cells, such as CD15 and CD30, although the proliferative potential of RS cells was reported to be lower than that of Hodgkin cells. 25 To date, markers that can differentiate Hodgkin cells from RS cells have not been established.We recently reported that HL cell lines, L1236 and L428, were composed of two types of cells: cells with a single nucleus (S) that resemble Hodgk...

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Section: Relationship Between Ros-low and Aldh-high Populationsmentioning

confidence: 99%

Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells

Ikeda

Mamat

Tian

et al. 2012

Laboratory Investigation

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“…ALDH isozymes including ALDH1A1, are important for multiple biological activities including drug resistance [25][26]. We have demonstrated that the uptake of TPT in cells exposed to an ALDH inhibitor (disulfiram) is enhanced (unpublished; data not shown).…”

Section: Extended Modelmentioning

confidence: 84%

Kinetic modelling of the role of the aldehyde dehydrogenase enzyme and the breast cancer resistance protein in drug resistance and transport

Atari

Chappell

Errington

et al. 2011

Computer Methods and Programs in Biomedicine

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Article Title: Kinetic modelling of the role of the aldehyde dehydrogenase enzyme and the breast cancer resistance protein in drug resistance and transport Year of publication: 2010Link to publication: http://www.cmpbjournal.com/home Link to published article: http://dx.doi. org/10.1016/j.cmpb.2010.06.008 Copyright statement: This is the author's version of a work that was accepted for publication in Computer Methods and Programs in Biomedicine. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Computer Methods and Programs in Biomedicine, [Vol.104, (No. Verification of the proposed model is achieved using scanning-laser microscopy data from live human breast cancer cells. Before estimating the unknown model parameters from the experimental in vitro data it is essential to determine parameter uniqueness (or otherwise) from this imposed output structure. This is formally performed as a structural identifiability analysis, which demonstrates that all of the unknown model parameters are uniquely determined by the output structure corresponding to the experiment.

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“…4) In regard to ALDH, results reported to date appear to be complicated. Its enzymatic activity has been demonstrated in SCLC and NSCLC cells by the Aldefluor assay, as well as by a spectrophotometrical assay [92][93][94][95][96][97]. On the other hand, an immunohistochemical analysis using antibodies against ALDH1A1 and ALDH3A1 in tissue sections of surgical specimens of lung cancer demonstrated the expression of these ALDH isozymes in NSCLC cases, but not in SCLC cases, suggesting that the ALDH protein expression was limited to CSCs in NSCLC [96].…”

Section: Brief Summary Of Lung Csc Markers and Potential Problemsmentioning

confidence: 99%

“…One important issue to be addressed is which ALDH isozymes are responsible for the ALDH activity used to identify stem cell progenitors. Several studies have demonstrated that ALDH activity is needed for the differentiation of primitive progenitors into mature cells, thus fulfilling one of the defining characteristics of multipotent stem cells, and some lines of evidence suggest that ALDH1A1 is an important marker of hematopoietic stem cell progenitors [92]. In fact, ALDH1A1 is one of the enzymes involved in the production of retinoic acid from retinol, and retinoic acid is considered significantly important in maintaining a balance between hematopoietic stem cell selfrenewal and differentiation [92].…”

Section: Brief Summary Of Lung Csc Markers and Potential Problemsmentioning

confidence: 99%

See 1 more Smart Citation

Cancer Stem Cells in Lung Cancer: Distinct Differences between Small Cell and Non-Small Cell Lung Carcinomas

Okihara¹,

Nagahara²,

Katayama³

et al. 2011

Cancer Stem Cells Theories and Practice

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ALDH isozymes downregulation affects cell growth, cell motility and gene expression in lung cancer cells

Abstract: Background: Aldehyde dehydrogenase isozymes ALDH1A1 and ALDH3A1 are highly expressed in non small cell lung cancer. Neither the mechanisms nor the biologic significance for such over expression have been studied.

Cited by 140 publications

References 64 publications

Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells

Reactive oxygen species and aldehyde dehydrogenase activity in Hodgkin lymphoma cells

Kinetic modelling of the role of the aldehyde dehydrogenase enzyme and the breast cancer resistance protein in drug resistance and transport

Cancer Stem Cells in Lung Cancer: Distinct Differences between Small Cell and Non-Small Cell Lung Carcinomas

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