Alendronate-Modified Nanoceria with Multiantioxidant Enzyme-Mimetic Activity for Reactive Oxygen Species/Reactive Nitrogen Species Scavenging from Cigarette Smoke

Abstract

Highly toxic radicals including reactive oxygen species (ROS) and reactive nitrogen species (RNS) in cigarette smoke play an important role in oxidative damage of the lungs, which cannot be efficiently scavenged by current filter techniques. Herein, a novel alendronate-coated nanoceria (CeAL) nanozyme is explored for cigarette filter modification for ROS/RNS scavenging. The CeAL nanozyme with an adjustable oxidation state and high thermal stability exhibits an excellent superoxide dismutase (SOD)-like activity, hydroxyl radical elimination capacity, catalase-mimicking activity, and nitric oxide radical scavenging ability. These synergistic antioxidant abilities make the CeAL nanozyme a lucrative additive for cigarette filters. The filter incorporated with the CeAL nanozyme can efficiently scavenge ROS/RNS in the hot smoke generated by burned commercial cigarettes, resulting in reduction of oxidative stress-induced pulmonary injury and acute inflammation of mice. The developed CeAL nanozyme opens up new opportunities for cigarette filter modification to decrease the toxicity of cigarette smoke and expands the application fields of nanoceria.

ROS-Targeted Depression Therapy via BSA-Incubated Ceria Nanoclusters

“…48 In addition, multiple studies have demonstrated better stability (in a wider range of pH and working temperature) and multiple ROS scavenging ability of nanoceria versus natural enzymes such as SOD and CAT. 28,49 Furthermore, compared with other CeO 2 nanoparticles, 23,32,50 CeO 2 @BSA nanoclusters possessed the smallest average size and better ROS scavenging ability (Supplementary Table 2). All these comparisons indicate excellent catalytic property to remove multiple ROS.…”

Preparation, Characterization and Multiple Biological Properties of Peptide-Modified Cerium Oxide Nanoparticles

“…X-ray photoelectron spectroscopy (XPS) analyses were used to determine the surface Ce 3+ /Ce 4+ ratio on the surface of the CeO 2 @PAA@RGD nanoparticles. Two main peaks, including Ce 3d 3/2 and Ce 3d 5/2, in the Ce 3d spectrum of CeO 2 @PAA@RGD are presented in Figure 1 e. The Ce 3d spectrum was decomposed into six peaks [ 49 ], including four peaks of Ce 4+ and two peaks of Ce 3+ [ 41 ]. The peaks of Ce 3+ are 881.90 and 900.60 eV, while the peaks of Ce 4+ at 885.30, 904.00, 887.10, and 906.30 eV.…”

“…The hydroxyl radicals (·OH) scavenging assay was performed according to the report by Zhou et al [ 41 ]. ·OH was produced by the Fenton reaction: H 2 O 2 + Fe 2+ = ·OH + H 2 O + Fe 3+ .…”

Thermosensitive Hydrogel Incorporating Prussian Blue Nanoparticles Promotes Diabetic Wound Healing via ROS Scavenging and Mitochondrial Function Restoration

“…39 The H 2 O 2 scavenging effect of PBNPs was examined by mixing H 2 O 2 (500 μM) and PBNPs (12.5, 25, 50, 100, and 200 μg/mL), with the residual H 2 O 2 levels in the supernatant being measured using a hydrogen peroxide assay kit and a microplate spectrophotometer by assessing absorbance examined at 562 nm. Hydroxyl radicals (•OH) elimination assay was conducted according to the report by Zhou et al 40 •OH in aqueous solution was generated through the Fenton reaction by adding FeSO 4 (0.4 mM) and H 2 O 2 (0.4 mM) into PBS (0.1 M, pH 7.4) at 37 °C. Then, different concentrations of PBNPs were added into the solution, followed by adding a salicylic acid (SA) indicator (2 mM) to detect the remaining •OH.…”