Alfa-glucosidase-inhibiting activity of some Mexican plants used in the treatment of type 2 diabetes

Andrade‐Cetto, Adolfo; Becerra-Jiménez, Jaime; Cárdenas-Vázquez, René

doi:10.1016/j.jep.2007.10.031

Cited by 146 publications

(88 citation statements)

References 16 publications

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“…This was done according to the method of Andrade-Cetto with slight modifications (Andrade-Cetto et al, 2008). The assay was carried out by incubating a solution of starch substrate (2% w/v maltose) 1 mL of 0.2 M Tris buffer pH 8.0 and various concentrations of different test samples for 5 min at 37 C. The reaction was initiated by adding 1 mL of a-glucosidase enzyme (1 U/mL) followed by incubation for 10 min at 37 C. The reaction mixture was heated for 2 min in a boiling water bath to stop the reaction.…”

Section: A-glucosidase Inhibition Assaymentioning

confidence: 99%

Antidiabetic and antioxidant potency evaluation of different fractions obtained fromCucumis prophetarumfruit

Gawli

Lakshmidevi²

2014

Pharmaceutical Biology

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Context: Cucumis prophetarum Linn. (Cucurbitaceae) fruit is used for inflammatory-related problems and is proved to be possessing anticancer and hepatoprotective effects. Objective: The present investigation was to study the effect of different fractions of C. prophetarum on antidiabetic and antioxidant activity. Materials and methods: Aqueous crude extract (CE) of C. prophetarum fruits was fractionated into water soluble fraction 1 (F1), chloroform fraction 2 (F2), basic fraction 3 (F3), and neutral fraction 4 (F4) by acid-base extraction. CE and its fractions at different doses (0.02-0.1 mg/mL) were subjected to antidiabetic (a-amylase and a-glucosidase inhibition assays) and antioxidant (DPPH, superoxide radical scavenging (SO) and metal chelation) evaluation. Results: F1 exhibited effective antidiabetic activity (p50.05) with an IC 50 value of 20.6 and 59.9 mg/mL. The activity decreased in the order of CE4F44F34F2, according to a-amylase assay, which were the same, with the exception of the rank order of F4 and CE, as the a-glucosidase assay. Furthermore, F1 (IC 50 ¼ 73 mg/mL) showed better reducing ability than CE4F44F24F3 (IC 50 ¼ 78-272 mg/mL), according to the DPPH assay. In SO and metal chelation assays, F1 showed the highest activity (IC 50 ¼ 101 and 147 mg/mL), respectively; the activity decreased in the order of CE4F44F34F2 (IC 50 ¼ 126-469 mg/mL) for SO and 194-944 mg/mL for metal chelation assay. Conclusion:The results indicate that F1 possesses potent in vitro antidiabetic and antioxidant activities.

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Section: A-glucosidase Inhibition Assaymentioning

confidence: 99%

Antidiabetic and antioxidant potency evaluation of different fractions obtained fromCucumis prophetarumfruit

Gawli

Lakshmidevi²

2014

Pharmaceutical Biology

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“…In general, the enzyme inhibitory activities of the plant extracts not only depend on the polyphenols quantity but also might depend on the quality [34]. That is the reason due to which ethyl acetate extract shows batter alpha amylase and alpha glucosidase enzyme inhibition than ethanol while ethyl acetate extract having lower total phenolic contents (table 6 and 7).…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Screening and Pharmacological Evaluation of Different Extracts of Plant Casearia Tomentosa Leaves

Tyagi

Shukla

2017

Int J Pharm Pharm Sci

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Objective: In present study different extracts of the leaves of plant Casearia tomentosa was investigated for the phytochemical screening, total phenolic content and various pharmacological activities like antioxidant, antidiabetic, antibacterial.Methods: Leaves of C. tomentosa were successively extracted by soxhlet extraction method using petroleum ether, chloroform, ethyl acetate and ethanol. The preliminary phytochemical investigation of different extracts was carried out according to standard methods. Antioxidant activity evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant potential (FRAP) method. Total phenolic content was evaluated by Folin-ciocalteau method. Antidiabetic activity estimated by α-amylase and α-glucosidase inhibitory assay while antibacterial activity was performed by disk diffusion method. Conclusion:The present study suggests that Casearia tomentosa leaves can be used as a potential source of natural antioxidant and natural antidiabetic, antibacterial agents.

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“…The amount of liberated glucose is measured by glucose oxidase peroxidase method and absorbance was measured at 510 nm. 29,30,31 The results of both were expressed as % inhibition calculated using the formula:…”

Section: Activities α-Amylase Inhibitory Activitymentioning

confidence: 99%

COMPARATIVE INHIBITORY POTENTIAL OF ASHODHITA (RAW) AND SHODHITA CURCUMA LONGA LINN ON Α -AMYLASE AND Α-Glucosidase

Kumar¹,

Thakur²,

Nariya³

et al. 2017

Int. J. Res. Ayurveda Pharm.

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Turmeric (Haridra) is one of the versatile herb for spices, condiment and anti-diabetic activity in Indian System of medicine and tropical country. To compare the in-vitro enzymatic inhibitory effect (α-amylase and α-glycosidase) for supporting purified (shodhita) and raw turmeric samples. Authenticate unprocessed and processed (ayurvedic classic) turmeric powder extracts prepared by maceration with methanol and subjected (in different level concentration) to the anti-hyperglycemic selective enzymatic activity and colour reaction was measured by UV-VIS spectroscopy. This study proves the alteration in the inhibitory effect of raw and shodhita samples of Haridra on α-amylase of IC50 in decreasing order TZ (113.72) < TW (119.22) < TR (121.861) < TT (161.35) < TGM (218.85) and α-glycosidase TZ (121.32) < TW (139.48) < TR (145.29) < TT (185.51) < TGM (276.12) respectively. Purified turmeric samples gives better effect than raw sample. In that way shodhan effect established indirectly synergistic and supraadditive effect of turmeric in context to antidiabetic.

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Alfa-glucosidase-inhibiting activity of some Mexican plants used in the treatment of type 2 diabetes

Cited by 146 publications

References 16 publications

Antidiabetic and antioxidant potency evaluation of different fractions obtained fromCucumis prophetarumfruit

Antidiabetic and antioxidant potency evaluation of different fractions obtained fromCucumis prophetarumfruit

Phytochemical Screening and Pharmacological Evaluation of Different Extracts of Plant Casearia Tomentosa Leaves

COMPARATIVE INHIBITORY POTENTIAL OF ASHODHITA (RAW) AND SHODHITA CURCUMA LONGA LINN ON Α -AMYLASE AND Α-Glucosidase

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