Antidiabetic and antioxidant potency evaluation of different fractions obtained fromCucumis prophetarumfruit

Abstract

Context: Cucumis prophetarum Linn. (Cucurbitaceae) fruit is used for inflammatory-related problems and is proved to be possessing anticancer and hepatoprotective effects. Objective: The present investigation was to study the effect of different fractions of C. prophetarum on antidiabetic and antioxidant activity. Materials and methods: Aqueous crude extract (CE) of C. prophetarum fruits was fractionated into water soluble fraction 1 (F1), chloroform fraction 2 (F2), basic fraction 3 (F3), and neutral fraction 4 (F4) by acid-base extraction. CE and its fractions at different doses (0.02-0.1 mg/mL) were subjected to antidiabetic (a-amylase and a-glucosidase inhibition assays) and antioxidant (DPPH, superoxide radical scavenging (SO) and metal chelation) evaluation. Results: F1 exhibited effective antidiabetic activity (p50.05) with an IC 50 value of 20.6 and 59.9 mg/mL. The activity decreased in the order of CE4F44F34F2, according to a-amylase assay, which were the same, with the exception of the rank order of F4 and CE, as the a-glucosidase assay. Furthermore, F1 (IC 50 ¼ 73 mg/mL) showed better reducing ability than CE4F44F24F3 (IC 50 ¼ 78-272 mg/mL), according to the DPPH assay. In SO and metal chelation assays, F1 showed the highest activity (IC 50 ¼ 101 and 147 mg/mL), respectively; the activity decreased in the order of CE4F44F34F2 (IC 50 ¼ 126-469 mg/mL) for SO and 194-944 mg/mL for metal chelation assay. Conclusion:The results indicate that F1 possesses potent in vitro antidiabetic and antioxidant activities.

Isolation of anticancer constituents from Cucumis prophetarum var. prophetarum through bioassay-guided fractionation

“…The presence of a singlet methyl signal at δ H 2.02 in 1 H NMR spectra and two carbon signals at 21.9 and 170.3 in 13 C NMR spectra indicated the presence of an acetate moiety at C-25. The above data indicated the presence of a cucurbitacin tetracyclic triterpene skeleton; a comparison of the data with those published [ 10 , 12 – 14 ] indicated that structure 2 was characterized as cucurbitacin B. Further confirmation of compound 2 was achieved by comparison with an authentic sample of cucurbitacin B from our lab.…”

“…Cucurbitacin (B, E, I, O, P and Q1); dihydrocucurbitacin (D and E), isocucurbitacin (B, D and E) and dihydroisocucurbitacin (D and E) have been reported to be isolated from Cucumis prophetarum L. Cucurbitacin B and Dihydrocucurbitacin B isolated from Cucumis prophetarum L., were studied for their cytotoxic activity towards human cancer cell lines, mouse embryonic fibroblast (NIH3T3) and virally transformed form (KA3IT) cells [ 9 ]. Recently the antidiabetic and antioxidant activity of the different fractions of fruits of Cucumis prophetarum L. has been reported [ 10 ].…”

High‐performance thin‐layer chromatography coupled attenuated total reflectance‐Fourier‐transform infrared and NMR spectroscopy‐based identification of α‐amylase inhibitor from the aerial part of Asparagus racemosus Willd

“…IC 50 is calculated as antioxidant or α ‐amylase inhibition concentration required to reduce the DPPH or α ‐amylase concentration by 50%. Consequently, the lower IC 50 value means the higher antioxidant or α ‐amylase inhibitory activity 38–40 . The correlation study confirmed the activity of plant extracts through chemical derivatisation.…”

“…Consequently, the lower IC 50 value means the higher antioxidant or α-amylase inhibitory activity. [38][39][40] The correlation study confirmed the activity of plant extracts through chemical derivatisation.…”

Phytochemical Investigation on the Root Extraction of Cucumis Prophetarum L.

“…Moreover, as cited by Abdulrhman Alsayari et al, 2018 the antidiabetic and antioxidant activity of the different fractions of fruits of Cucumis prophetarum L has been reported [16].…”