Algal filtrate: a low cost substitute to synthetic growth regulators for direct organogenesis of embryo culture in Jatropha curcas (Ratanjyot)

Shrivastava, Sarita; Banerjee, Meenakshi

doi:10.1007/s11738-009-0355-7

Cited by 8 publications

(8 citation statements)

References 17 publications

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“…In medium containing cyanobacterial fi ltrate, shoot and root lengths were comparatively higher than the other treatments with synthetic plant growth regulators. The same trend was found for chlorophylls a and b (Shrivastava and Banerjee 2009 ). Somatic embryos were generated using different explants like cotyledonary leaf and leaf callus with different regeneration effi ciencies.…”

Section: Multiple Shoot Bud Inductionsupporting

confidence: 56%

Micropropagation of Plants

Singh

2015

Plant Biology and Biotechnology

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Micropropagation is a rapid multiplication of a selected plant using in vitro culture techniques. In this chapter various aspects of micropropagation have been discussed. The propagation of selected plant through micropropagation would be useful for raising plantation using apical and nodal segment. They are best for micropropagation and mostly result in true to type plants. These segments upon the subsequent subcultures result in a number of multiple shoots. These multiple shoots on elongation allowed to root in vitro. After rooting, they are in vitro hardened and transferred to fi eld. The potential of plant tissue culture is well recognized, as it increases agricultural production and generates rural employment. But the high cost of production on micropropagation is a major bottleneck. Low-cost protocol development can popularize this method. High multiplication rate, use of low-cost chemicals, high rooting and survival percentage, and use of ex vitro rooting method can minimize the expense of any protocol. Presently, both the developing and the developed countries require lowcost technologies to progressively reduce the cost of production. The performance of tissue plants generally better than cutting and seedling. Initially, some morphological changes occur in micropropagated plant, but in the course of time, they minimize. Somaclonal variation caused during callus cultures can be used to generate variants for hybridization. Variation in phenotype of plants produced in tissue culture can be characterized as either temporary or permanent. Temporary variation includes increased branching, greater susceptibility to disease, and lack of uniform response. Micropropagated plants need to be monitored to get long-term data for its

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Section: Multiple Shoot Bud Inductionsupporting

confidence: 56%

Micropropagation of Plants

Singh

2015

Plant Biology and Biotechnology

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“…The development of axillary nodes from explants was also performed on MS medium containing 4.90 M IBA and 13.31 M of BA [22]. Citric acid has been recognized as a very efficient anti-oxidant compound in in vitro culture [23,24]. It prevents the production of polyphenols which often hinder organogenesis and cause explant necrosis.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Micropropagation of Jatropha curcas L. from Bud Aggregates

Mve

Mergeai

Druart

et al. 2013

J. Technol. Innov. Renew. Energy

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Entire plants were regenerated from nodes explants of Jatropha curcas L. following a procedure of bud aggregate induction on MS (Murashige and Skoog) medium supplemented with 25 mg.l -1 citric acid, 12.2 mg.l -1 adenine sulfate, 15 mg.l -1 L-arginine, 2.46 M IBA (indole-3-butyric acid), 30 g.l -1 sucrose and 7 g.l -1 of agar, and enriched with different balances of BA (benzyladenine) and L-glutamine. The histological studies performed on aggregates showed that the buds result from both the development of axillary buds and adventitious budding starting from underlying tissues of the explant. The culture medium containing 6.65 M BA and 25 mg.l -1 L-glutamine gave the best results with an average of 64 buds per aggregate after three weeks for all accessions tested. The buds developed into shoots when placed in an MS medium supplemented with 2.21 M BA, 5.70 M IAA (indole-3-acetic acid) and 15 mg.l -1 L-arginine. These shoots were isolated and then rooted in MS containing 2.46 M of IBA, 2% sucrose and 0.7% agar. The entire process took 13 weeks with a 98% survival rate in terms of plantlets acclimatization. We obtained a multiplication rate of 13 buds per explant and per subculture which is the double of those obtained in other recent works based on the micropropagation of J. curcas from node explants. This protocol is economically more profitable.

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“…The increase was less in A. thaliana grown on CM/1.25 (123% of control). Spent media of Aulosira fertilissima also boosted chlorophyll a and b content in Jatropha curcas, accompanied by an increase of photosynthetic efficiency (Shrivastava and Banerjee, 2009). …”

Section: Resultsmentioning

confidence: 95%

“…Preliminary studies indicate that algal extracellular exudates are indeed able to stimulate the growth of plant cells, even in a phylogenetically distinct higher plant such as Silene vulgaris (Caryophyllaceae) (Hanus-Fajerska et al, 2009) or in Daphne species (Wiszniewska et al, 2013). Previous studies have examined the effects on higher plants of compound(s) released into medium by ubiquitously occurring phytoplankton in suspension cell cultures and organ or embryo cultures (Hanus-Fajerska et al, 2009;Shrivastava and Banerjee, 2009;Gurusaravanan et al, 2013). Our present work is the first demonstration of the effect of Desmodesmus subspicatus-conditioned medium on the growth of whole model plants (Nicotiana tabacum, Arabidopsis thaliana) cultured in vitro.…”

Section: Introductionmentioning

confidence: 99%

Growth Improvement of Nicotiana and Arabidopsis In Vitro by Microalgal Conditioned Media

Zielińska¹,

Matusiak-Mikulin²,

Grabski³

et al. 2015

Acta Biologica Cracoviensia S. Botanica

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Conditioned medium (CM) is a general term describing media in which cells have already been cultivated for some time. Such media, usually clarified by filtration, have been used by plant biotechnologists as additives supporting the growth of cell suspensions, organs and whole plants. This study examined the effect of CM obtained from green alga Desmodesmus subspicatus on the growth and functioning of the photosynthetic apparatus of Nicotiana tabacum and Arabidopsis thaliana in culture in vitro. Plants where cultured on CM diluted 1.25-, 2-and 5-fold with MS medium. The increase in fresh and dry weight was highest in tobacco and Arabidopsis cultured on CM/2 and CM/1.25 media. Those two concentrations also increased the amount of chlorophylls in both plants tested. CM improved parameter PI (reflecting the photosynthetic "vitality" of the organism) and electron transport efficiency, and increased the fraction of active reaction centers. Analysis of chlorophyll fluorescence in vivo suggests that the improvement of these plants grown in the presence of algal CM may result from stimulation of photosynthesis. Algal CM offers a convenient, cheap, universal supplement for stimulating the growth of higher plants in vitro.K Ke ey y w wo or rd ds s: : Algal exudates, in vitro culture, growth improvement, photosynthesis.

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Algal filtrate: a low cost substitute to synthetic growth regulators for direct organogenesis of embryo culture in Jatropha curcas (Ratanjyot)

Cited by 8 publications

References 17 publications

Micropropagation of Plants

Micropropagation of Plants

In Vitro Micropropagation of Jatropha curcas L. from Bud Aggregates

Growth Improvement of Nicotiana and Arabidopsis In Vitro by Microalgal Conditioned Media

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