Algal polysaccharide’s potential to combat respiratory infections caused by Klebsiella pneumoniae and Serratia marcescens biofilms

Vishwakarma, Jyoti; Waghela, Bhumika; Falcao, Berness; Vavilala, Sirisha L.

doi:10.1007/s12010-021-03632-7

Cited by 12 publications

(7 citation statements)

References 77 publications

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“…The supernatant was gently discarded and the pellet was air dried. The weights of the pellets of both untreated and Ts-AgNPs-treated samples were measured and the results were represented as a graph of the total weight of EPS against the concentration of Ts-AgNPs [ 32 ].…”

Section: Methodsmentioning

confidence: 99%

Tryptone-stabilized silver nanoparticles’ potential to mitigate planktonic and biofilm growth forms of Serratia marcescens

et al. 2022

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Several microbial pathogens are capable of forming biofilms. These microbial communities pose a serious challenge to the healthcare sector as they are quite difficult to combat. Given the challenges associated with the antibiotic-based management of biofilms, the research focus has now been shifted towards finding alternate treatment strategies that can replace or complement the antibacterial properties of antibiotics. The field of nanotechnology offers several novel and revolutionary approaches to eradicate biofilm-forming microbes. In this study, we evaluated the antibacterial and antibiofilm efficacy of in-house synthesized, tryptone-stabilized silver nanoparticles (Ts-AgNPs) against the superbug Serratia marcescens . The nanoparticles were of spherical morphology with an average hydrodynamic diameter of 170 nm and considerable colloidal stability with a Zeta potential of − 24 ± 6.15 mV. Ts-AgNPs showed strong antibacterial activities with a minimum inhibitory concentration (MIC 50 ) of 2.5 µg/mL and minimum bactericidal concentration (MBC) of 12.5 µg/mL against S. marcescens . The nanoparticles altered the cell surface hydrophobicity and inhibited biofilm formation. The Ts-AgNPs were also effective in distorting pre-existing biofilms by degrading the extracellular DNA (eDNA) component of the extracellular polymeric substance (EPS) layer. Furthermore, reduction in quorum-sensing (QS)-induced virulence factors produced by S. marcescens indicated that Ts-AgNPs attenuated the QS pathway. Together, these findings suggest that Ts-AgNPs are an important anti-planktonic and antibiofilm agent that can be explored for both the prevention and treatment of infections caused by S. marcescens . Graphical abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00775-022-01977-w.

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Section: Methodsmentioning

confidence: 99%

Tryptone-stabilized silver nanoparticles’ potential to mitigate planktonic and biofilm growth forms of Serratia marcescens

et al. 2022

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“…The sulphated polysaccharides produced by green algae Chlamydomonas reinhardtii (Cr-SPs) also showed antibacterial and antibiofilm potential against K. pneumoniae (MTCC no. 432) (Vishwakarma et al, 2022). Cr-SPs treatment eradicated more than 50% of preformed biofilm at 0.5 mg/mL and removed 100% biofilm at 4 mg/mL and 8 mg/mL by reducing EPS production and eDNA content of the bacteria.…”

Section: Microbial and Their Metabolitesmentioning

confidence: 99%

Relationship between biofilm formation and antibiotic resistance of Klebsiella pneumoniae and updates on antibiofilm therapeutic strategies

Li,

Gao,

et al. 2024

Front. Cell. Infect. Microbiol.

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Klebsiella pneumoniae is a Gram-negative bacterium within the Enterobacteriaceae family that can cause multiple systemic infections, such as respiratory, blood, liver abscesses and urinary systems. Antibiotic resistance is a global health threat and K. pneumoniae warrants special attention due to its resistance to most modern day antibiotics. Biofilm formation is a critical obstruction that enhances the antibiotic resistance of K. pneumoniae. However, knowledge on the molecular mechanisms of biofilm formation and its relation with antibiotic resistance in K. pneumoniae is limited. Understanding the molecular mechanisms of biofilm formation and its correlation with antibiotic resistance is crucial for providing insight for the design of new drugs to control and treat biofilm-related infections. In this review, we summarize recent advances in genes contributing to the biofilm formation of K. pneumoniae, new progress on the relationship between biofilm formation and antibiotic resistance, and new therapeutic strategies targeting biofilms. Finally, we discuss future research directions that target biofilm formation and antibiotic resistance of this priority pathogen.

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“…In this experiment, eDNA was isolated from control and treated cells by a modified protocol as mentioned in Vishwakarma and Vavilala 2020. Purified e-DNA was quantified spectrophotometrically by using the absorbance ratio of A260/ A280 [68,69].…”

Section: Extracellular Dna (Edna) Quantificationmentioning

confidence: 99%

Exploring the efficacy of tryptone-stabilized silver nanoparticles against respiratory tract infection-causing bacteria: a study on planktonic and biofilm forms

Pandey,

Pradhan,

Meher

et al. 2024

Biomed. Mater.

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Respiratory tract infections are a common cause of mortality and morbidity in the human population. The overuse of antibiotics to overcome such infections has led to antibiotic resistance. The emergence of Multidrug resistant (MDR) bacteria is necessitating the development of novel therapeutic techniques in order to avoid a major global clinical threat. Our study aims to investigate the potential of Tryptone stabilised silver nanoparticles (Ts-AgNPs) on planktonic and biofilms produced by Klebsiella pneumoniae and Pseudomonas aeruginosa. The MIC50 of Ts-AgNPs was found to be as low as 1.7 μg/mL and 2.7 μg/mL for K. pneumoniae and P.aeruginosa respectively. Ts-AgNPs ability to alter redox environment by producing intracellular ROS, time-kill curves showing substantial decrease in the bacterial growth and significantly reduced colony forming units further validate its antimicrobial effect. The biofilm inhibition and eradication ability of Ts-AgNPs was found to be as high as 93% and 97% in both the tested organisms. A significant decrease in the eDNA and EPS quantity in Ts-AgNPs treated cells proved its ability to successfully distort the matrix and matured biofilms. Interestingly Ts-AgNPs also attenuated QS-induced virulence factors production,. This study paves way to develop Ts-AgNPs as novel antibiotics against respiratory tract infections causing bacterial biofilms.

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Algal polysaccharide’s potential to combat respiratory infections caused by Klebsiella pneumoniae and Serratia marcescens biofilms

Cited by 12 publications

References 77 publications

Tryptone-stabilized silver nanoparticles’ potential to mitigate planktonic and biofilm growth forms of Serratia marcescens

Tryptone-stabilized silver nanoparticles’ potential to mitigate planktonic and biofilm growth forms of Serratia marcescens

Relationship between biofilm formation and antibiotic resistance of Klebsiella pneumoniae and updates on antibiofilm therapeutic strategies

Exploring the efficacy of tryptone-stabilized silver nanoparticles against respiratory tract infection-causing bacteria: a study on planktonic and biofilm forms

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