Alginate-based microcapsules generated with the coaxial electrospray method for clinical application

Barron, Catherine; He, Jia‐Qiang

doi:10.1080/09205063.2017.1318030

Cited by 20 publications

(11 citation statements)

References 34 publications

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“…Barron and He reported that the most prominent and successful method in clinical application was the coaxial electrospray method whose system consisted of two syringe pumps for pushing the cell-containing fluid and the alginate solution, leading to a microcapsule composed of an aqueous core fluid with living cells surrounded by an alginate hydrogel shell [34]. Basta et al.…”

Section: Discussionmentioning

confidence: 99%

The intraperitoneal space is more favorable than the subcutaneous one for transplanting alginate fiber containing iPS-derived islet-like cells

Fukuda

Yabe

Nishida

et al. 2019

Regenerative Therapy

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Introduction Although immunosuppressants are required for current islet transplantation for type 1 diabetic patients, many papers have already reported encapsulation devices for islets to avoid immunological attack. The aim of this study is to determine the optimal number of cells and optimal transplantation site for human iPS-derived islet-like cells encapsulated in alginate fiber using diabetic model mice. Methods We used a suspension culture system for inducing islet-like cells from human iPS cells throughout the islet differentiation process. Islet-like spheroids were encapsulated in the alginate fiber, and cell transplantation experiments were performed with STZ-induced diabetic NOD/SCID mice. We compared the efficacy of transplanted cells between intraperitoneal and subcutaneous administration of alginate fibers by measuring blood glucose and human C-peptide levels serially in mice. Grafts were analyzed histologically, and gene expression in pancreatic β cells was also compared. Results We demonstrated the reversal of hyperglycemia in diabetic model mice after intraperitoneal administration of these fibers, but not with subcutaneous ones. Intraperitoneal fibers were easily retrieved without any adhesion. Although we detected human c-peptide in mice plasma after subcutaneous administration of these fibers, these fibers became encased by fibrous tissue. Conclusions These results suggest that the intraperitoneal space is favorable for islet-like cells derived from human iPS cells when encapsulated in alginate fiber.

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Section: Discussionmentioning

confidence: 99%

The intraperitoneal space is more favorable than the subcutaneous one for transplanting alginate fiber containing iPS-derived islet-like cells

Fukuda

Yabe

Nishida

et al. 2019

Regenerative Therapy

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“…On the other hand, EHDA has been increasingly used for high throughput production of particles in micro/nano scale with uniform size and morphology. In this method, a voltage is applied to cut the droplets from the needle tip before they fall as a result of gravity [15]. This technique is of great interest due to its easiness to operate, versatility, cytocompatibility, high efficiency, and the ability to work in a sterile environment [15–17].…”

Section: Introductionmentioning

confidence: 99%

Cell encapsulation in liquified compartments: Protocol optimization and challenges

Correia

Ghasemzadeh-Hasankolaei

Mano

2019

PLoS ONE

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Cell encapsulation is a widely used technique in the field of Tissue Engineering and Regenerative Medicine (TERM). However, for the particular case of liquefied compartmentalised systems, only a limited number of studies have been reported in the literature. We have been exploring a unique cell encapsulation system composed by liquefied and multilayered capsules. This system transfigured the concept of 3D scaffolds for TERM, and was already successfully applied for bone and cartilage regeneration. Due to a number of appealing features, we envisage that it can be applied in many other fields, including in advanced therapies or as disease models for drug discovery. In this review, we intend to highlight the advantages of this new system, while discussing the methodology, and sharing the protocol optimization and results. The different liquefied systems for cell encapsulation reported in the literature will be also discussed, considering the different encapsulation matrixes as core templates, the types of membranes, and the core liquefaction treatments.

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“…Usually, the setup for collecting the particles produced by electrospraying is a hard surface located on the counter electrode. Nevertheless, different authors have published an alternative “soft” collecting approach where the droplets or particles are caught in liquid medium [ 222 , 223 , 224 , 225 ]. One of the benefits of this collecting method is the prevention of possible particle aggregation or film formation on the hard collector [ 223 ].…”

Section: Pharmaceutical Applicationsmentioning

confidence: 99%

“…Due to the difference in surface tension of the various collection liquids, the impaction force and thus degree of deformation changes, resulting in a variety of different particle shapes [ 225 ]. Another application of a liquid collector is described by Barron et al, who reviewed multiple applications of alginate-based microencapsulation by the coaxial electrospray method [ 224 ]. A coaxial nozzle allows for two different fluids to be electrosprayed, where the core fluid is encapsulated by the outer shell fluid.…”

Section: Pharmaceutical Applicationsmentioning

confidence: 99%

“…In this case, an alginate solution is found in the shell and the core is composed of an aqueous fluid containing living cells. When the alginate solution reaches the liquid collector containing a salt solution (e.g., CaCl 2 ), immediate gelling of the alginate will take place, producing core-shell microcapsules with an alginate hydrogel shell [ 224 ]. Nikoo et al utilized a similar alginate system, but operated in the dripping mode with a single nozzle for the generation of calcium alginate microhydrogels and investigated various process and formulation parameters on the final characteristics of the particles [ 226 ].…”

Section: Pharmaceutical Applicationsmentioning

confidence: 99%

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Unraveling Particle Formation: From Single Droplet Drying to Spray Drying and Electrospraying

et al. 2020

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Spray drying and electrospraying are well-established drying processes that already have proven their value in the pharmaceutical field. However, there is currently still a lack of knowledge on the fundamentals of the particle formation process, thereby hampering fast and cost-effective particle engineering. To get a better understanding of how functional particles are formed with respect to process and formulation parameters, it is indispensable to offer a comprehensive overview of critical aspects of the droplet drying and particle formation process. This review therefore closely relates single droplet drying to pharmaceutical applications. Although excellent reviews exist of the different aspects, there is, to the best of our knowledge, no single review that describes all steps that one should consider when trying to engineer a certain type of particle morphology. The findings presented in this article have strengthened the predictive value of single droplet drying for pharmaceutical drying applications like spray drying and electrospraying. Continuous follow-up of the particle formation process in single droplet drying experiments hence allows optimization of manufacturing processes and particle engineering approaches and acceleration of process development.

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Alginate-based microcapsules generated with the coaxial electrospray method for clinical application

Cited by 20 publications

References 34 publications

The intraperitoneal space is more favorable than the subcutaneous one for transplanting alginate fiber containing iPS-derived islet-like cells

The intraperitoneal space is more favorable than the subcutaneous one for transplanting alginate fiber containing iPS-derived islet-like cells

Cell encapsulation in liquified compartments: Protocol optimization and challenges

Unraveling Particle Formation: From Single Droplet Drying to Spray Drying and Electrospraying

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