Alginate-based microencapsulation of retinal pigment epithelial cell line for cell therapy

Wikström, Jonna; Elomaa, Matti; Syväjärvi, Heli; Kuokkanen, Johanna; Yliperttula, Marjo; Honkakoski, Paavo; Urtti, Arto

doi:10.1016/j.biomaterials.2007.10.056

Cited by 61 publications

(43 citation statements)

References 19 publications

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“…Transplantation of encapsulated cells in spherical-shaped devices (microbeads or microcapsules) is an appealing strategy for treatments of a wide variety of diseases such as cancer, diabetes, Parkinson's and other endocrine disorders [1][2][3]. Living cells embedded in microbeads, acting as scaffolds, have also found application in other fields, including cell culture and tissue engineering [4].…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of polysaccharidic microbeads by a microfluidic technique: Application to the encapsulation of Sertoli cells

Capretto¹,

Mazzitelli²,

Luca³

et al. 2010

Acta Biomaterialia

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Section: Introductionmentioning

confidence: 99%

Preparation and characterization of polysaccharidic microbeads by a microfluidic technique: Application to the encapsulation of Sertoli cells

Capretto¹,

Mazzitelli²,

Luca³

et al. 2010

Acta Biomaterialia

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“…Alginate is an unbranched natural copolymer composed of β-D-mannuronate (M) and α-L-guluronate (G), linked together by 1,4 bonds (Garbayo et al, 2002;Bajpai and Sharma, 2004). Due to its relative stability, biocompatibility, adjustable porosity and simplicity of use, alginate is thus a biomaterial of choice when it comes to entrapping cells (Garbayo et al, 2002;Zmora et al, 2002;David et al, 2004a;De Vos et al, 2006;Zimmermann et al, 2007;Wikstrom et al, 2008), to cell therapy (Chang, 2005;Paul et al, 2009) or to being used in medical devices (Ueyama et al, 2002;Orive et al, 2004;Gao et al, 2005;De Vos et al, 2006;Orive et al, 2006;Wikstrom et al, 2008). Some of these studies were conducted with hepatocytes that were either encapsulated within alginate beads (Selden et al, 1999;David et al, 2004b;Gao et al, 2005;Kinasiewicz et al, 2007;Kinasiewicz et al, 2008) or capsules (Canaple et al, 2001;Orive et al, 2004;Haque et al, 2005), or seeded within alginate scaffolds (Zmora et al, 2002;Seo et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Impact of alginate type and bead diameter on mass transfers and the metabolic activities of encapsulated C3A cells in bioartificial liver applications

Gautier

Carpentier²,

Dufresne³

et al. 2011

eCM

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Liver-assist devices have been developed in the last few decades to support patients with liver failure on the road to recovery or transplantation. Fluidised bed bio-artificial livers -where liver cells are encapsulated within alginate beads -appear to be a valuable alternative to hollow fibre devices for improving mass transfers and enhancing treatment efficacy. This approach nevertheless deserves optimization in terms of bead production. The aim of this study was to investigate the impact of alginate type and of two bead diameters (1000 μm and 600 μm) on mass transfers within beads and on the biological functions of encapsulated C3A cells.After assessing the effect of the encapsulation process on bead quality, we investigated cell viability and metabolic activities (ammonia, albumin, α-foetoprotein synthesis and glucose consumption). They were successfully maintained over 48 h within fluidised bed bioreactors, independently of alginate type and bead diameter. Mass transfers were not significantly influenced by the latter parameters. Finally, suggestions are made for improving the entrapment process as a means of enhancing the treatment efficiency of the fluidised bed bioartificial liver.

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“…3 Several studies have sought to understand the relationship between alginate composition and the function of the graft. 4,[17][18][19][20][21][22][23][24] Two common themes emerge in the literature regarding alginate composition and graft performance: the effect of the M=G content of the alginate and the importance of a polycation coating layer. These two variables have been related to gel mechanical stability, viability of encapsulated cells, in vivo biocompatibility, and diffusion through the alginate gel.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4] Encapsulation protects graft cells from potential damage caused by an immune response while allowing the secretion of therapeutic agents from the cells into the surrounding host tissue. Small molecules such as glucose, oxygen, and waste products freely pass through the gel matrix.…”

Section: Introductionmentioning

confidence: 99%

Alginate Composition Effects on a Neural Stem Cell–Seeded Scaffold

Purcell

Singh

Kipke

2009

Tissue Engineering Part C: Methods

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The purpose of this study was to evaluate the effects of alginate composition on the neurotrophic factor release, viability, and proliferation of encapsulated neural stem cells (NSCs), as well as on the mechanical stability of the scaffold itself. Four compositions were tested: a high guluronic acid (68%) and a high mannuronic acid (54%) content alginate, with or without a poly-L-lysine (PLL) coating layer. Enzyme-linked immunosorbent assay was used to quantify the release of brain-derived neurotrophic factor, glial-derived neurotrophic factor, and nerve growth factor from the encapsulated cells. All three factors were detected from encapsulated cells only when a high L-guluronic acid alginate without PLL was used. Additionally, capsules with this composition remained intact more frequently when exposed to solutions of low osmolarity, potentially indicating superior mechanical stability. Alginate beads with a PLL-coated, high D-mannuronic acid composition were the most prone to breakage in the osmotic pressure test, and were too fragile for histology and proliferation assays after 1 week in vitro. NSCs survived and proliferated in the three remaining alginate compositions similarly over the 21-day study course irrespective of scaffold condition. NSC-seeded alginate beads with a high L-guluronic acid, non-PLL-coated composition may be useful in the repair of injured nervous tissue, where the mechanism is the secretion of neuroprotective factors. We verify the neuroprotective effects of medium conditioned by NSCseeded alginate beads on the serum withdrawal-mediated death of PC-12 cells here.

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Alginate-based microencapsulation of retinal pigment epithelial cell line for cell therapy

Cited by 61 publications

References 19 publications

Preparation and characterization of polysaccharidic microbeads by a microfluidic technique: Application to the encapsulation of Sertoli cells

Preparation and characterization of polysaccharidic microbeads by a microfluidic technique: Application to the encapsulation of Sertoli cells

Impact of alginate type and bead diameter on mass transfers and the metabolic activities of encapsulated C3A cells in bioartificial liver applications

Alginate Composition Effects on a Neural Stem Cell–Seeded Scaffold

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