Alginate–chitosan coacervation in production of artificial seeds

Tay, Ling-Fong; Khoh, Leng-Khim; Loh, Chiang‐Shiong; Khor, Eugene

doi:10.1002/bit.260420407

Cited by 35 publications

(11 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another aspect of micropropagation where chitin-based products have proved effective is in the breeding of hybrid varieties. Chitosan has successfully been used as a component of artificial seed coats that are required for the embryo rescue procedures when breeding certain hybrid plant varieties [216].…”

Section: Use Of Chitin-based Treatments In Non-field Agricultural Sysmentioning

confidence: 99%

A Review of the Applications of Chitin and Its Derivatives in Agriculture to Modify Plant-Microbial Interactions and Improve Crop Yields

2013

View full text Add to dashboard Cite

Abstract:In recent decades, a greater knowledge of chitin chemistry, and the increased availability of chitin-containing waste materials from the seafood industry, have led to the testing and development of chitin-containing products for a wide variety of applications in the agriculture industry. A number of modes of action have been proposed for how chitin and its derivatives can improve crop yield. In addition to direct effects on plant nutrition and plant growth stimulation, chitin-derived products have also been shown to be toxic to plant pests and pathogens, induce plant defenses and stimulate the growth and activity of beneficial microbes. A repeating theme of the published studies is that chitin-based treatments augment and amplify the action of beneficial chitinolytic microbes. This article reviews the evidence for claims that chitin-based products can improve crop yields and the current understanding of the modes of action with a focus on plant-microbe interactions.

show abstract

Section: Use Of Chitin-based Treatments In Non-field Agricultural Sysmentioning

confidence: 99%

A Review of the Applications of Chitin and Its Derivatives in Agriculture to Modify Plant-Microbial Interactions and Improve Crop Yields

2013

View full text Add to dashboard Cite

show abstract

“…So far, chitosan and its derivatives have been extensively studied and widely utilized in medicine, wastewater treatment and in biotechnological applications [1–3]. In agriculture, chitosan has been used for agroproduct preservation, seed coating, fertilizer and artificial seeds [4], owing to its antimicrobial activity [5] and plant‐growth‐stimulation properties [1]. Chitosan has been reported to induce the activity of chitinase and enhance seed germination rates and crop yield [6–8] and also to reduce the transpiration of plants [9].…”

Section: Introductionmentioning

confidence: 99%

Biological effect of irradiated chitosan on plants in vitro

Luân

Nagasawa

et al. 2005

Biotech and App Biochem

View full text Add to dashboard Cite

For degradation of chitosan, chitosan with an 80% degree of deacetylation and a weight-average molecular mass (Mw) of approx. 48 kDa was irradiated with gamma-rays at doses up to 200 kGy in a 10% (w/v) solution. The Mw of chitosan was reduced from 48 to 9.1 kDa by irradiation. The characteristics of irradiated chitosan were analysed by using Fourier-transform IR spectroscopy and an elemental analyser. The amino group was found to be stable, whereas the C-O-C group decreased with increase in the dose. The product of chitosan irradiated at 100 kGy with an Mw of approx. 16 kDa showed the strongest growth promotion effect on plants in vitro. For shoot culture, supplementation with irradiated chitosan increased the fresh biomass of shoot clusters (7.2-17.0%) as well as the shoot multiplication rate (17.9-69.0%) for Chrysanthemum morifolium (florist's chrysanthemum), Limonium latifolium (limonium or sea-lavender), Eustoma grandiflorum (lisianthus, tulip gentian or Texas bluebell) and Fragaria ananassa (modern garden strawberry). The optimum concentrations of irradiated chitosan were found to be approx. 70-100 mg/l for chrysanthemum, 50-100 mg/l for lisianthus and 30-100 mg/l for limonium. For the plantlet culture, the optimum concentrations were found to be approx. 100 mg/l for chrysanthemum, 30 mg/l for lisianthus, 40 mg/l for limonium and 50 mg/l for strawberry. Supplementation with optimum concentrations of irradiated chitosan resulted in a significant increase in the fresh biomass (68.1% for chrysanthemum, 48.5% for lisianthus, 53.6% for limonium and 26.4% for strawberry), shoot height (19.4% for chrysanthemum, 16.5% for lisianthus, 33.9% for limonium and 25.9% for strawberry) and root length (40.6% for chrysanthemum, 66.9% for lisianthus, 23.4% for limonium and 22.6% for strawberry). In addition, treatment with irradiated chitosan enhanced the activity of chitosanase in treated plants and also improved the survival ratio and growth of the transferred plantlets acclimatized for 10-30 days under greenhouse conditions.

show abstract

“…Redenbaugh et al (1986) considered the development of a suitable encapsulation matrix as an important criterion for the commercial application of artificial seeds. The de facto approach for obtaining artificial seeds has been the ionotropic gelation of sodium alginate by calcium ions containing somatic embryos (Tay et al, 1993). The advantages of sodium alginate include excellent water solubility at room temperature and the use of divalent metal salts for gelation by complex coacervation, obviating the need for thermal treatment.…”

Section: Introductionmentioning

confidence: 99%

“…The limitations of sodium alginate include the tacky nature of the capsules (Redenbaugh et al, 1988) and rapid dehydration (Dainty et al, 1986), and their high po-rosity results in marked cell and nutrient leakage (Redenbaugh et al, 1988). Artificial seeds made from a single alginate matrix are also susceptible to phosphate chelation because of the reversibility of the gel network and are therefore unstable in buffer solutions and culture media containing high concentrations of electrolytes (Tay et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Two‐coat systems for encapsulation of Spathoglottis plicata (Orchidaceae) seeds and protocorms

Khor

Loh

1998

Biotechnol. Bioeng.

Self Cite

View full text Add to dashboard Cite

Complex coacervation of alginate-chitosan and alginate-gelatin were used to develop two-coat systems for the encapsulation of Spathoglottis plicata seeds and protocorms (top-shaped structures formed after seed germination of orchids). Both the seeds and the protocorms could withstand the encapsulation treatments with high viability. About 54% of seeds and 40% of large protocorms (1.6-2.0 mm) were able to tolerate a 6-h desiccation treatment. However, viability of the small protocorms (0.7-0.9 mm) was greatly reduced if they were desiccated before encapsulation. Encapsulation after desiccation significantly increased the percentage viability of seeds and protocorms. Treatment with abscisic acid (ABA, 10 −5 M) before desiccation and encapsulation resulted in high percentage viability in seeds and large protocorms whereas the small protocorms were found to be less tolerant to the treatments.

show abstract

Alginate–chitosan coacervation in production of artificial seeds

Cited by 35 publications

References 10 publications

A Review of the Applications of Chitin and Its Derivatives in Agriculture to Modify Plant-Microbial Interactions and Improve Crop Yields

A Review of the Applications of Chitin and Its Derivatives in Agriculture to Modify Plant-Microbial Interactions and Improve Crop Yields

Biological effect of irradiated chitosan on plants in vitro

Two‐coat systems for encapsulation of Spathoglottis plicata (Orchidaceae) seeds and protocorms

Contact Info

Product

Resources

About