Alginate Encapsulation of Begonia Microshoots for Short‐Term Storage and Distribution

Abstract

Synthetic seeds were formed from shoot tips of two in vitro grown Begonia cultivars using 3% sodium alginate in Murashige and Skoog medium (MS) salt solution as the gel matrix and 100 mM calcium chloride for complexation. Synthetic seed formation was achieved by releasing the sodium alginate/explant combination into 100 mM calcium chloride (CaCl2 ·H2O) solution for 30 or 45 min. Both control and encapsulated shoots were transferred into sterile Petri dishes and stored at 4°C or 22°C for 0, 2, 4, 6, or 8 weeks. Conversion of synthetic seeds into plantlets for both storage environments was assessed in MS medium or peat-based substrate. No significant difference was found between the 30 and 45 min CaCl2 ·H2O treatments or the two cultivars. Encapsulation of explants improved survival rate over time irrespective of the medium type or storage environment. Survival rates of 88, 53, 28, and 11% for encapsulated microshoots versus 73, 13, 0, and 0% for control explants were achieved in microshoots stored for 2, 4, 6, and 8 weeks, respectively. The best results were obtained when synthetic seeds were stored at 4°C and germinated on MS medium. Regenerated plantlets were successfully established in potting soil.

Encapsulation of Shoot Tips and Nodal Segments for in Vitro Storage of “Kober 5BB” Grapevine Rootstock

“…In cryopreservation, this technique has been used for different species [32][33][34]. Moreover, various studies have described encapsulation for short and medium conservation (from 1 month to 1 year) without losing explant viability [35][36][37][38] and for increasing the interval between subcultures by reducing growth.…”

In Vitro Assessment of Antioxidant Effect of Begonia Rex Putz. Leaf Extract on Oxidative Stress Biomarkers In The Equine Erythrocytes Model

“…The first living plant in Begonia was introduced to Europe during the eighteenth century, and thereafter over 400 natural species have been introduced for horticulture and many cultivars have been developed (Tebbitt, 2005). Begonias are among the most popular ornamental plants in the world thanks to their large, showy, and long-lasting multicolor flowers, ranging from white to pink, red, and yellow (Sakhanokho et al, 2013;Twyford et al, 2014). They are used as garden plants and potted plants, in hanging baskets, and as greenhouse flowers, as well as potherbs or leaf vegetables in many parts of the world.…”

“…They are used as garden plants and potted plants, in hanging baskets, and as greenhouse flowers, as well as potherbs or leaf vegetables in many parts of the world. The roots and tubers of some species have been reported to possess antimicrobial activities and are used to treat various ailments (Sakhanokho et al, 2013).…”

Encapsulation and Germination of Synthetic Seeds of Chrysanthemum

“…In a decade later, encapsulation of nodal segments with Caalginate to develop artificial seeds has been investigated in many plant species [1,[4][5][6][7]. It also developing in many horticulture plants research published such as Begonia [8], strawberry [9], Chrysanthemum [10], Oxalis [11] etc. The research on encapsulation technology is currently mainly carried out with a focus on the development of its technology, constrains and commercial prospects for the future [12].…”