Alginate–lavender nanofibers with antibacterial and anti-inflammatory activity to effectively promote burn healing

Abstract

This is an Accepted Manuscript, which has been through the Royal Society of Chemistry peer review process and has been accepted for publication.Accepted Manuscripts are published online shortly after acceptance, before technical editing, formatting and proof reading. Using this free service, authors can make their results available to the community, in citable form, before we publish the edited article. We will replace this Accepted Manuscript with the edited and formatted Advance Article as soon as it is available.You can find more information about Accepted Manuscripts in the Information for Authors. One of the current challenges in wound care is the development of multifunctional dressings that can both protect the wound from external agents and promote the regeneration of the new tissue. Here, we show the combined use of two naturally derived compounds, sodium alginate and lavender essential oil, for the production of bioactive nanofibrous dressings by electrospinning, and their efficacy for the treatment of skin burns induced by midrange ultraviolet radiation (UVB). We demonstrate that the engineered dressings reduce the risk of microbial infection of the burn, since they stop the growth of Staphylococcus aureus. Furthermore, they are able to control and reduce the inflammatory response that is induced in human foreskin fibroblasts by lipopolysaccharides, and in rodents by UVB exposure. In particular, we report a remarkable reduction of pro-inflammatory cytokines when fibroblasts or animals are treated with the alginate-based nanofibers. The down-regulation of cytokines production and the absence of erythema on the skin of the treated animals confirm that the here described dressings are promising as advanced biomedical devices for burn management.

Electrospun nanofibrous materials for wound healing applications

“…Hajiali et al [58] incorporated lavender oil (LO) into sodium alginate and PEO (SA-PEO/LO) nanofibers and demonstrated their efficiency in antibacterial and treatment of skin burns induced by midrange ultraviolet radiation (UVB). The LO release measurement indicated that therefore, the produced alginate-based nanofibers were active for more than 2 days and able to release the antibacterial and antiinflammatory agent.…”

Electrospinning of natural polymers for advanced wound care: towards responsive and adaptive dressings

“…Thus, the solidification of the filament takes place and a network of randomly distributed non-woven fibres is collected on the grounded target (Figure 2b and 2c). 41,42 Thanks to its high flexibility in terms of materials that can be processed and types of fibrous scaffolds that can be produced, the electrospinning technology has attracted enormous research and industrial interests in the last years. Natural and synthetic polymers, their blends and nano-composites can be easily structured by ES, resulting in fibres with engineered physicochemical properties.…”

“…For the cure of UV-induced skin burns, instead, nanofibers of alginate-PEO and lavender essential oil have been used. 41 The composite mats exhibited antibacterial activity against S. aureus and anti-inflammatory properties, being able to reduce the production of pro-inflammatory cytokines both in vitro and in vivo. The skin of the treated animals recovered faster than in the case of untreated ones, and no appearance of erythema was detected.…”

Fumarate-loaded electrospun nanofibers with anti-inflammatory activity for fast recovery of mild skin burns

“…[8,9] Typically, electrospun nanofibers produced forburn treatments are loaded with active agents, such as natural compounds, essential oils or drugs. [10,11]Derivatives of fumaric acid, such as monomethyl fumarate (MMF), dimethyl fumarate (DMF) and polypropylene fumarate (PPF), are also attractive materials in wound healing for their anti-inflammatory activity. MMF and DMF as well as PPF, have been already proposed for biomedical purposes.…”

“…MMF and DMF as well as PPF, have been already proposed for biomedical purposes. [10,11] In particular, MMF and DMF can be used for the treatment of human immunodeficiency virus (HIV) because they induce a modulation of immuno and antioxidant responses. [14] This property has been studied by Gill and Kolson [14] in order to limit the HIV disease progression.…”