Alginate Lyase Promotes Diffusion of Aminoglycosides through the Extracellular Polysaccharide of Mucoid<i>Pseudomonas aeruginosa</i>

Hatch, Richard A.; Schiller, Neal L.

doi:10.1128/aac.42.4.974

Cited by 159 publications

(99 citation statements)

References 41 publications

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“…Pseudomonas aeruginosa biofilms are particularly resistant to antibiotic treatment owing to the production and deposition of alginate in the matrix. For example, a 2% solution of alginate from a P. aeruginosa biofilm significantly inhibited the diffusion of gentamicin and tobramycin [31]. Alginate is a polyanionic exopolysaccaride and tends to concentrate divalent cations thus reducing the antimicrobial efficacy of aminoglycosides and tetracyclines [32].…”

Section: Discussionmentioning

confidence: 99%

In vitro susceptibility of established biofilms composed of a clinical wound isolate of Pseudomonas aeruginosa treated with lactoferrin and xylitol

Ammons

Ward²,

Fisher

et al. 2009

International Journal of Antimicrobial Agents

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The medical impact of bacterial biofilms has increased with the recognition of biofilms as a major contributor to chronic wounds such as diabetic foot ulcers, venous leg ulcers and pressure ulcers. Traditional methods of treatment have proven ineffective, therefore this article presents in vitro evidence to support the use of novel antimicrobials in the treatment of Pseudomonas aeruginosa biofilm. An in vitro biofilm model with a clinical isolate of P. aeruginosa was subjected to treatment with either lactoferrin or xylitol alone or in combination. Combined lactoferrin and xylitol treatment disrupted the structure of the P. aeruginosa biofilm and resulted in a >2log reduction in viability. In situ analysis indicated that while xylitol treatment appeared to disrupt the biofilm structure, lactoferrin treatment resulted in a greater than two-fold increase in the number of permeabilised bacterial cells. The findings presented here indicated that combined treatment with lactoferrin and xylitol significantly decreases the viability of established P. aeruginosa biofilms in vitro and that the antimicrobial mechanism of this treatment includes both biofilm structural disruption and permeablisation of bacterial membranes.

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Section: Discussionmentioning

confidence: 99%

In vitro susceptibility of established biofilms composed of a clinical wound isolate of Pseudomonas aeruginosa treated with lactoferrin and xylitol

Ammons

Ward²,

Fisher

et al. 2009

International Journal of Antimicrobial Agents

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“…Effect of shear stress on the formation of bacterial biofilm in a microfluidic channel Aeri Park 1 , Heon-Ho Jeong 1 , Jintae Lee 2 , Keun Pil Kim 3 & Chang-Soo Lee 1 als, temperature, and flow rate 12,13 .…”

Section: Original Researchmentioning

confidence: 99%

Effect of shear stress on the formation of bacterial biofilm in a microfluidic channel

et al. 2011

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Biofilms form an irregular network matrix that is surrounded by extracellular polymeric substrate (EPS). The architecture of biofilm plays an important role in protecting bacteria under physical, chemical, and biological stress. The shear stress is one of the major factors to construct stable bioflim. The experimental observation of biofilm formation on large-scale water flow has been limited because most of fluid pipe are water and sewer lines. This study presents the biofilm formation in a PDMS-based microfluidic channel which is able to simulate fluid pipes at small scale. We could characterize the hydrodynamics of the growth of single-species bacteria between biofilm formation and the external environmental factors. Particularly, the dynamics of biofilm formation confirms that biofilm, under the optimum shear stress, efficiently form a stable EPS structure which provides a mechanical shield against high-pressure fluidic flow.

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“…It has emerged as a dominant pulmonary pathogen with biofilm-forming capability, resulting in progressive and intractable chronic pulmonary infections, especially in patients with cystic fibrosis [2]. There is also an increasing awareness of the important role of P. aeruginosa biofilms in the contamination of medical biomaterials such as catheters and prostheses [1]. Biofilm infections are difficult to eradicate with antimicrobial treatment, and in vitro susceptibility tests show considerable resistance of biofilm cells to killing.…”

Section: Introductionmentioning

confidence: 99%

“…Pseudomonas aeruginosa is one of the most important opportunistic human pathogens [1]. It has emerged as a dominant pulmonary pathogen with biofilm-forming capability, resulting in progressive and intractable chronic pulmonary infections, especially in patients with cystic fibrosis [2].…”

Section: Introductionmentioning

confidence: 99%

Bactericidal activity of various antibiotics against biofilm-producing Pseudomonas aeruginosa

Ali¹,

Mohammadimehr²,

Alaei³

2006

International Journal of Antimicrobial Agents

121

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Alginate Lyase Promotes Diffusion of Aminoglycosides through the Extracellular Polysaccharide of MucoidPseudomonas aeruginosa

Cited by 159 publications

References 41 publications

In vitro susceptibility of established biofilms composed of a clinical wound isolate of Pseudomonas aeruginosa treated with lactoferrin and xylitol

In vitro susceptibility of established biofilms composed of a clinical wound isolate of Pseudomonas aeruginosa treated with lactoferrin and xylitol

Effect of shear stress on the formation of bacterial biofilm in a microfluidic channel

Bactericidal activity of various antibiotics against biofilm-producing Pseudomonas aeruginosa

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