Alicyclobacillus consociatus sp. nov., isolated from a human clinical specimen

Abstract

A Gram-stain-positive, aerobic organism, isolated from a blood sample from a 51-year-old woman, was studied for its taxonomic position. Based on 16S rRNA gene sequence similarity comparisons, strain CCUG 53762T was grouped into the genus Alicyclobacillus, most closely related to the type strain of Alicyclobacillus pohliae (94.7 %). The 16S rRNA gene sequence similarity to other species of the genus Alicyclobacillus was ¡91 % and similarity to species of the genus Tumebacillus was 91.3-93 %. The occurrence of menaquinone MK-7 as the major respiratory quinone, meso-diaminopimelic acid as the diagnostic diamino acid of the cell wall and the fatty acid profile supported the allocation of the strain to the genus Alicyclobacillus. Major fatty acids were iso-and anteiso-branched fatty acids. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unknown phospholipids. The absence of the iso-branched fatty acids iso-C 16 : 0 and iso-C 17 : 0 allowed differentiation of strain CCUG 53762 T from A. pohliae CIP 109385T . In addition, the results of physiological and biochemical tests also allowed phenotypic differentiation of strain CCUG 53762T from this most closely related species. The G+C content of the DNA was 47 mol%.Strain CCUG 53762 T therefore represents a novel species of the genus Alicyclobacillus, for which we propose the name Alicyclobacillus consociatus sp. nov., with CCUG 53762 T (5CCM Goto et al., 2002Goto et al., , 2007. The genus Alicyclobacillus is remarkably heterogeneous. Initially described as a genus characterized chemotaxonomically by the presence of large amounts of alicyclic fatty acids (Wisotzkey et al. 1992), many species have since been described, among them Alicyclobacillus aeris, Alicyclobacillus ferrooxydans, Alicyclobacillus pomorum, Alicyclobacillus macrosporangiidus, Alicyclobacillus contaminans and Alicyclobacillus pohliae (Goto et al., 2007;Guo et al., 2009;Imperio et al., 2008;Jiang et al., 2008), in which these characteristic fatty acids have not been reported.In 2007, an endospore-forming organism was isolated from a blood sample from a 51-year-old woman on blood agar at 37 u C. Unfortunately, no other information of the clinical relevance of the strain is available. The strain was isolated from a blood culture, but it is not clear, and no proof has been documented, whether the strain really was the causal agent of an infection.The organism was subcultivated on R2A agar (Oxoid) at 30 u C for 24 h. Gram-staining was performed according to the Hucker method as described by Gerhardt et al. (1994). Cell morphology was observed under a light microscope (Zeiss) at a magnification of 61000 using cells that had been grown for 3 days at 25 uC on R2A agar. Cells of strain CCUG 53762 T were rod-shaped (0.8-1.0 mm wide and 2-5 mm long, sometimes forming filamentous cells) and (rarely) formed terminal spherical endospores, grew strictly aerobically and formed beige, circular and convex colonies on the surface of R2A agar. Cells were no...

Spoilage by Alicyclobacillus Bacteria in Juice and Beverage Products: Chemical, Physical, and Combined Control Methods

“…Up to now 20 species, 2 subspecies, and 2 genomic species (a genomic species is a possible new species without a formal name, because the data were not sufficient to support the proposal of a new species or subspecies when it was published) of Alicyclobacillus have been identified, including 1 species newly reported in May 2013 (Glaeser and others ). The first isolating sources and some important physiological and biochemical characteristics of all species in the genus Alicyclobacillus are summarized in Table .…”

“…All these species are Gram‐positive (or variable, usually in old cultures) and aerobic, except A. sendaiensis (Gram‐negative) (Tsuruoka and others ) and A. pohliae (facultatively anaerobic sometimes). Many species cannot live under 30 or 40 °C, but A. acidiphilus (Matsubara and others ), A. acidoterrestris (Wisotzkey and others ), A. consociatus (Glaeser and others ), A. disulfidooxidans (Dufresne and others ), A. fastidiosus (Goto and others ), A. ferrooxydans (Jiang and others ), and A. tolerans (Karavaiko and others ) can grow under 30 °C, and A. disulfidooxidans can even grow at 4 °C. Most species can live above 50 °C (up to 70 °C), but A. consociatus , A. disulfidooxidans , and A. ferrooxydans cannot grow at temperatures above 50 °C.…”

Mesonia hippocampi sp. nov., isolated from the brood pouch of a diseased Barbour's Seahorse (Hippocampus barbouri)

“…For further genotypic analyses, high molecular mass genomic DNA was extracted as described by Pitcher et al (1989). The genomic DNA G+C content was determined by the DNA melting temperature method established by Gonzalez & Saiz-Jimenez (2002) as described previously (Glaeser et al , 2013). The genomic DNA G+C content determined for 96_Hippo_TS_3/13 T was 30 mol%, which was in the range of G+C content reported for type strains of members of the genus Mesonia (30–42.1 %; Lee et al , 2012).…”

Characterization of the N2O-producing soil bacterium Rhizobium azooxidifex sp. nov.

“…For chemotaxonomic characterization of the isolated strain, the G+C content of the genomic DNA was determined as described previously (Glaeser et al, 2013). The DNA melting temperature was used according to the method established by Gonzalez & Saiz-Jimenez (2002).…”