Mesonia hippocampi sp. nov., isolated from the brood pouch of a diseased Barbour's Seahorse (Hippocampus barbouri)

Abstract

An orange-pigmented, Gram-staining-negative, rod-shaped bacterium, designated 96_Hippo_TS_3/13 T was isolated from the brood pouch of a diseased seahorse male of the species Hippocampus barbouri from the animal facility of the University of Giessen, Germany. Phylogenetic analyses based on the nearly full-length 16S rRNA gene sequence placed strain 96_Hippo_TS_3/13 T into the monophyletic cluster of the genus Mesonia within the family Flavobacteriaceae. However, the strain shared only 92.2-93.8 % sequence similarity to type strains of species of the genus Mesonia, with highest sequence similarity to the type strain of Mesonia aquimarina. Cellular fatty acid analysis showed a Mesonia-typical fatty acid profile including several branched and hydroxyl fatty acids with highest amounts of iso-C 15 : 0 (40.9 %) followed by iso-C 17 : 0 3-OH (14.8 %). In the polyamine pattern, sym-homospermidine was predominant. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. The quinone system contained exclusively menaquinone MK-6. The only identified compound in the polar lipid profile was phosphatidylethanolamine present in major amounts. Additionally, major amounts of an unidentified aminolipid and two unidentified lipids not containing a phosphate group, an amino group or a sugar residue were detected. The genomic G+C content of strain 96_Hippo_TS_3/13 T was 30 mol%. Based on genotypic, chemotaxonomic and physiological characterizations we propose a novel species of the genus Mesonia, Mesonia hippocampi sp. nov., with strain 96_Hippo_TS_3/13 T (5CIP 110839 T 5 LMG 28572 T 5CCM 8557 T ) as the type strain. An emended description of the genus Mesonia is also provided.

Mesonia sediminis sp. nov., isolated from a sea cucumber culture pond

“…The composition of the major fatty acids and polar lipids is the typical pattern which is described for the genus (Kolberg et al 2015), although there were differences in the proportions. pH range for growth 6.0-10.5 5.5-8.5 6.0-9.5 6.5-12.5 5.5-9.5 6.1-9.1 6.0-9.5 Tolerance to NaCl (%) 0.5-7 0-12 1-15 1-10 0.…”

“…phycicola (Kang and Lee 2010), M. ostreae , M. aquimarina (Choi et al 2015) and M. hippocampi (Kolberg et al 2015). Members of the genus Mesonia have been isolated from marine related environments and are described as Gram-stain negative, thin-rod shaped bacteria forming yellow colonies and do not for spores.…”

“…Seawater or Na ? ions are reported to be a prerequisite for growth (Kolberg et al 2015). Some species of the genus Mesonia can produce flexirubin pigments (Kolberg et al 2015).…”

“…ions are reported to be a prerequisite for growth (Kolberg et al 2015). Some species of the genus Mesonia can produce flexirubin pigments (Kolberg et al 2015). MK-6 is the major respiratory quinone and straightand branched-chain unsaturated fatty acids are the predominant fatty acids (Nedashkovskaya and Kim 2011).…”

Paramesonia marina gen. nov., sp. nov., isolated from deep-sea water of the Indian Ocean

“…Strains of the genus Mesonia Nedashkovskaya et al 2003 [19] emend. Lee et al 2012 [20] described as moderately halophilic require NaCl or sea water for growth and, according to those studies, all of the type strains of the genus Mesonia were isolated from diverse marine environments [19][20][21][22][23][24][25]. The species of Mesonia were characterized as above and also include strictly aerobic bac-teria that do not form endospores.…”