Mesonia sediminis sp. nov., isolated from a sea cucumber culture pond

Abstract

A yellow-pigmented, Gram-stain negative and facultatively anaerobic bacterium, designated MF326T , was isolated from a sample of sediment collected from a sea cucumber culture pond in Rongcheng, China (122°14 0 34 00 E 36°54 0 36 00 N). Cells of strain MF326T were found to be catalase negative and oxidase positive. Optimal growth was found to occur at 30°C and pH 7.0-7.5 in the presence of 2.0-3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MF326 T is a member of the genus Mesonia and exhibits the high sequence similarity (94.3 %) with the type strain of Mesonia ostreae, followed by Mesonia algae (93.9 %).The dominant fatty acids of strain MF326T were identified as iso-C 15:0 , an unidentified fatty acid with an equivalent chain-length of 13.565 and anteiso-C 15:0 . The major polar lipids were found to be two unidentified lipids and phosphatidylethanolamine. The major respiratory quinone was found to be MK-6 and the genomic DNA G?C content was determined to be 40.7 mol%. On the basis of the phylogenetic analysis and differential phenotypic characteristics, it is concluded that strain MF326 T (=KCTC 42255 T =MCCC 1H00125 T ) should be assigned to the genus Mesonia as the type strain of a novel species, for which the name Mesonia sediminis sp. nov. is proposed.

Paramesonia marina gen. nov., sp. nov., isolated from deep-sea water of the Indian Ocean

“…The respiratory quinone of strain K7 T was determined to be MK-6 (100%). This was consistent with the species of the genus Mesonia [16,[19][20][21][22][23][24][25]. Polar lipids were extracted using a chloroform/methanol system and analysed using one-and two-dimensional TLC, as described previously [46].…”

“…Considering the 16S rRNA gene similarity of lower than 94.4 % to those of the close genera, the isolate cannot Table 2. Differential characteristics of strain K7 T and some phylogenetically related genera Strains: 1, K7 T ; 2, Mesonia, data from eight species (Nedashkovskaya et al [19,23], Choi et al [21], Kolberg et al [22], Sung et al [16], Lee et al [20], Kang and Lee [24], Wang et al [25] and this study); 3, Salinimicrobium, data from eight species (Lim et al [52], Zhang et al [14], Lee et al [48], Nedashkovskaya et al [53], Subhash et al [54], Kim et al [55] and Chen et al [56]); 4, Salegentibacter, data from ten species (Nedashkovskaya et al [49,57,58], Siamphan and Kim [15], Xia et al [59], Ivanova et al [60], Yoon et al [61,62], McCammon and Bowman [63] and Liang et al [64]); 5, Zunongwangia data from four species (Shao et al [13], Fidalgo et al [65], Rameshkumar et al [50] and Qin et al [66]). 6, Leeuwenhoekiella, data from six species (Nedashkovskaya et al [47,67], Pinhassi et al [68], Liu et al [17], Si et al [69] and this study); Major quinone and major polar lipids of all species were MK-6 and phosphatidylethanolamine, respectively.…”

“…Strains of the genus Mesonia Nedashkovskaya et al 2003 [19] emend. Lee et al 2012 [20] described as moderately halophilic require NaCl or sea water for growth and, according to those studies, all of the type strains of the genus Mesonia were isolated from diverse marine environments [19][20][21][22][23][24][25]. The species of Mesonia were characterized as above and also include strictly aerobic bac-teria that do not form endospores.…”

“…The G+C content of the genomic DNA is in the range 30-42.1 mol% [16]. At the time of writing, the genus Mesonia was comprised of eight species [16,[19][20][21][22][23][24][25]. All the type strains of the genus Mesonia were isolated from marine environments.…”

Genome Sequencing of Mesonia algae K4-1 Reveals Its Adaptation to the Arctic Ocean

“…Currently, a total of 8 species of Mesonia have been validly described (Nedashkovskaya et al, 2003, 2006; Kang and Lee, 2010; Lee et al, 2012; Choi et al, 2015; Kolberg et al, 2015; Wang et al, 2015; Sung et al, 2017). All species isolated from the ocean and some additional species can hydrolyze gelatin (Nedashkovskaya et al, 2003; Sung et al, 2017), casein (Nedashkovskaya et al, 2003; Choi et al, 2015; Sung et al, 2017), fucoidan (Urvantseva et al, 2006), Tween 80 (Nedashkovskaya et al, 2006; Kang and Lee, 2010; Choi et al, 2015; Wang et al, 2015). Earlier studies found that some strains of the genus can form biofilms (Jeong et al, 2016) and are multiresistant (Miranda et al, 2015).…”