Aligned electrospun fiber film loaded with multi-enzyme mimetic iridium nanozymes for wound healing

Abstract

A film with elaborate microstructures that offers biomimetic properties and multi functionalities is highly desired in wound healing. Here, we develop an aligned hydrogel fiber film integrated with multi-active constituents to promote wound healing. Such fiber films are designed and constructed by photo-crosslinking the methacrylate gelatin (GelMA) doped with silver nanoparticles (Ag NPs) and iridium nanoparticles coated with polyvinylpyrrolidone (PVP-Ir NPs) in the precursor solution using electrospinning. The nature of GelMA hydrogel and the aligned arrangement of nanofibers endow the film with high-water content, self-degradability, improved bionic characteristics, oriented cell growth, and improved cell proliferation and migration. Moreover, the encapsulated nanozymes and Ag NPs offer the fiber film with superior reactive oxygen species (ROS) scavenging and antibacterial capability. The infected wound model shows that the multi-active hydrogel fiber film can reduce inflammation by killing bacteria and decomposing ROS, which accelerates the growth of new blood vessels and granulation tissue. Benefitting from these features, the versatile aligned GelMA fiber film demonstrates the clinically translational potential for wound healing.

A hybrid nanoassembly for ultrasound-inducible cytosolic siRNA delivery and cancer sono-gene therapy

“…Moreover, whether this nanoassembly can be used to deliver other genetic drugs such as CRISPR/Cas9 [34] warrants further investigations. It will also be interesting to explore the combination of sonosensitizers with alternative biomaterials [35] for ultrasound-enhanced drug delivery.…”

Sequential Regulation of Local Reactive Oxygen Species by Ir@Cu/Zn-MOF Nanoparticles for Promoting Infected Wound Healing

“…In the experiments, the poly(N-vinylpyrrolidone)-coated iridium(iii) nanozymes (Ir-PVP) were synthetic based on the previous reports with minor adjustments. 25 Iridium trichloride (IrCl 3 ) was selected to react with PVP through stirring, rotary evaporation, and drying to frame the black production. TEM, XRD, and FT-IR results displayed the successful preparation of Ir-PVP (Figure S1).…”

“…22,23 In addition to its unique antioxidant, the Ir(III) complex has good activity against bacteria and low cytotoxicity against mammalian cells, making it also a green antibacterial material. 24,25 In this study, we successfully incorporated Cu 2+ and Ir-PVP into Zn-MOF to fabricate a core−shell Ir@Cu/Zn-MOF. This novel structure enables the simultaneous implementation of synergistic dual-ion antibacterial therapy and maintenance of redox homeostasis, resulting in infected wound treatment (Figure 1).…”

“…Iridium (Ir) nanoparticles have been used in anticancers, antibacterials, and treatment of acute kidney injury due to their diverse mimetic enzymatic properties, including catalase (CAT), peroxidase (POD), and oxidase (SOD). , It has been reported that synthetic polyvinylpyrrolidone-coated iridium nanozymes (Ir-PVP NPs) have multienzyme simulation properties and effectively remove multiple toxic reactive nitrogen and oxygen substances (RONS), including H 2 O 2 , •O 2– , •OH, and DPPH. , In addition to its unique antioxidant, the Ir­(III) complex has good activity against bacteria and low cytotoxicity against mammalian cells, making it also a green antibacterial material. , …”

Protective effect of bioactive iridium nanozymes on high altitude-related hypoxia-induced kidney injury in mice

“…Therefore, they have been widely used in biological detection and disease treatment in recent years. Iridium nanoparticles (Ir-NPs) possess both SOD- and CAT-like activities ( Liang et al, 2022 ), and are effective reactive oxygen/nitrogen species scavengers in treating metastatic breast tumors, wound healing, and acute kidney injury ( Zhang et al, 2021a ; Wang et al, 2022b ; Wu et al, 2022 ). However, the role of Ir-NPs in acute altitude kidney injury treatment has not yet been reported.…”