Aligned Scaffolds with Biomolecular Gradients for Regenerative Medicine

Li, Xiaoran; Chen, Zhenni; Zhang, Haimin; Zhuang, Yan; Shen, He; Chen, Yanyan; Zhao, Yannan; Chen, Bing; Xiao, Zhifeng

doi:10.3390/polym11020341

Cited by 34 publications

(18 citation statements)

References 91 publications

(106 reference statements)

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“…[ 41 ] These observations supported that integrated aligned nanofiber topography and gradient chemokine cues directed MSCs from the cell pool at the periphery to the central region synergistically. [ 42 ]…”

Section: Resultsmentioning

confidence: 99%

Programmable Building of Radially Gradient Nanofibrous Patches Enables Deployment, Bursting Bearing Capability, and Stem Cell Recruitment

Yao

Wang

et al. 2021

Adv Funct Materials

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Fibrous patches capable of withstanding bursting force and recruiting endogenous stem cells are of great demand for wound treatment. A programmable strategy for development of radially gradient nanofibrous patches with rapid deployment property, robust bursting bearing capability, and excellent mesenchymal stem cell (MSC) recruitment capability, is demonstrated. Benefiting from the royal water lily‐like radially branched architecture, the gradient fibrous (GF) patches exhibit fast deployment in aqueous solution (2 s), high bursting strength of 4.6 N, as well as “center‐to‐periphery” gradient immobilization of stromal‐cell‐derived factor 1α (SDF1α). The SDF1α gradient patches direct MSC migration from the periphery to the center along the aligned nanofibers, resulting in a 4.2 times higher migrated cell number and 2.6 times greater maximum migration distance than random fibrous patches with homogenous SDF1α. The gelatin methacryloyl coated GF patches respond to matrix metalloproteinase‐9 for “on‐demand” release of anti‐inflammatory drug diclofenac sodium (DS). Furthermore, repair of the mouse full‐thickness skin incision validates that SDF1α/DS/GF patches are able to provide feasible microenvironment to attenuate inflammation and improve endogenous MSC recruitment, leading to accelerated wound healing. This work may open a new pathway for development of smart tough fibrous patches for stimulating endogenous repair mechanisms during tissue regeneration.

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Section: Resultsmentioning

confidence: 99%

Programmable Building of Radially Gradient Nanofibrous Patches Enables Deployment, Bursting Bearing Capability, and Stem Cell Recruitment

Yao

Wang

et al. 2021

Adv Funct Materials

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“…Aiming for the stiffness of the native human ACL which is documented between 111-396 N/mm [6,63], a higher overall breaking load can be realized with PCL, since more fibers can be used before the adequate stiffness is exceeded. PCL has been extensively investigated in the scope of tendon and ligament tissue engineering in the form of nanofibrous scaffolds [27][28][29][30][31][32]35]. For high-load applications, such as the human ACL, the rotator cuff or the Achilles tendon, a lack of scalability towards application-related strength is limiting the transferability of nanofibrous scaffolds for high-load applications.…”

Section: Discussionmentioning

confidence: 99%

“…PCL is used as a material for tissue engineering of tendons, bone and cartilage in form of nanofibers, foams and 3D printed structures [22]. Existing strategies for tendon and ligament scaffolds are manifold, including numerous studies on nanofibrous and yarn-based structures [25][26][27][28][29][30][31][32]. Given the high mechanical demands associated with tendons and ligaments, textile structures are considered promising to replace anisotropic tissues [26,33].…”

Section: Introductionmentioning

confidence: 99%

Melt-Spun, Cross-Section Modified Polycaprolactone Fibers for Use in Tendon and Ligament Tissue Engineering

Bauer

Emonts

Bonten

et al. 2022

Fibers

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Tissue Engineering is considered a promising route to address existing deficits of autografts and permanent synthetic prostheses for tendons and ligaments. However, the requirements placed on the scaffold material are manifold and include mechanical, biological and degradation-related aspects. In addition, scalable processes and FDA-approved materials should be applied to ensure the transfer into clinical practice. To accommodate these aspects, this work focuses on the high-scale fabrication of high-strength and highly oriented polycaprolactone (PCL) fibers with adjustable cross-sectional geometry and degradation kinetics applying melt spinning technology. Four different fiber cross-sections were investigated to account for potential functionalization and cell growth guidance. Mechanical properties and crystallinity were studied for a 24-week exposure to phosphate-buffered saline (PBS) at 37 °C. PCL fibers were further processed into scaffolds using multistage circular braiding with three different hierarchical structures. One structure was selected based on its morphology and scaled up in thickness to match the requirements for a human anterior cruciate ligament (ACL) replacement. Applying a broad range of draw ratios (up to DR9.25), high-strength PCL fibers with excellent tensile strength (up to 69 cN/tex) could be readily fabricated. The strength retention after 24 weeks in PBS at 37 °C was 83–93%. The following braiding procedure did not affect the scaffolds’ mechanical properties as long as the number of filaments and the braiding angle remained constant. Up-scaled PCL scaffolds resisted loads of up to 4353.88 ± 37.30 N, whilst matching the stiffness of the human ACL (111–396 N/mm). In conclusion, this work demonstrates the fabrication of highly oriented PCL fibers with excellent mechanical properties. The created fibers represent a promising building block that can be further processed into versatile textile implants for tissue engineering and regenerative medicine.

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“…To promote wound healing, small molecular drugs and biomolecules [29,30] including hemostatic, anti-inflammatory and analgesic drugs, as well as growth factors have been loaded into electrospun nanofibers [31]. Notably, the emergence of stimuli-responsive drug delivery system have opened a door for generating intelligent wound dressings [32].…”

Section: Introductionmentioning

confidence: 99%

Electrospun Nanofibrous Materials for Wound Healing

et al. 2020

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Wound dressing materials which are capable of meeting the demands of accelerating wound closure and promoting wound healing process have being highly desired. Electrospun nanofibrous materials show great application potentials for wound healing owing to relatively large surface area, better mimicry of native extracellular matrix, adjustable waterproofness and breathability, and programmable drug delivery process. In this review article, we begin with a discussion of wound healing process and current commercial wound dressing materials. Then, we emphasize on electrospun nanofibrous materials for wound dressing, covering the efforts for controlling fiber alignment and morphology, constructing 3D scaffolds, developing waterproof-breathable membrane, governing drug delivery performance, and regulating stem cell behavior. Finally, we finish with challenges and future prospects of electrospun nanofibrous materials for wound dressings.

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Aligned Scaffolds with Biomolecular Gradients for Regenerative Medicine

Cited by 34 publications

References 91 publications

Programmable Building of Radially Gradient Nanofibrous Patches Enables Deployment, Bursting Bearing Capability, and Stem Cell Recruitment

Programmable Building of Radially Gradient Nanofibrous Patches Enables Deployment, Bursting Bearing Capability, and Stem Cell Recruitment

Melt-Spun, Cross-Section Modified Polycaprolactone Fibers for Use in Tendon and Ligament Tissue Engineering

Electrospun Nanofibrous Materials for Wound Healing

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