Alkali salt stress causes fast leaf apoplastic alkalinization together with shifts in ion and metabolite composition and transcription of key genes during the early adaptive response of Vicia faba L

Sagervanshi, Amit; Geilfus, Christoph‐Martin; Kaiser, Hartmut; Mühling, Karl H.

doi:10.1016/j.plantsci.2022.111253

Cited by 7 publications

(9 citation statements)

References 69 publications

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“…Therefore, previously reported contamination data obtained with traditional destructive AWF extraction methods are valid references to validate the new AWF extraction method, e.g. our own studies on V. faba (Shahzad et al, 2013;Sagervanshi et al, 2022) and common bean (O'Leary et al, 2016).…”

Section: /41mentioning

confidence: 63%

“…In the apoplast, K + concentration is significantly lower being about 10 mM [e.g. 3 to 12 mM in V. faba (Lohaus et al, 2001;Sagervanshi et al, 2022); to our knowledge, no data available for Arabidopsis]. Figure 2 shows information about both symplastic marker, K + concentration and G6PDH-activity, in leaf AWFs that were collected from controland salt-treated V. faba and Arabidopsis leaves.…”

Section: Non-destructively Sampled Awfs Show Minimal Symplastic Conta...mentioning

confidence: 99%

“…Contamination markers such as K + (Lohaus et al, 2001) and the activity of symplastic enzymes such as malate dehydrogenase (MDH) and glucose-6-phosphate dehydrogenase (G6PDH) are frequently used (Baker et al, 2012;Boudart et al, 2005;O'Leary et al, 2014). AWFs that were collected from non-stressed plant leaves using the traditional 'infiltrationcentrifugation' technique showed K + concentrations between about 3 to 12 mM K + in V. faba (Lohaus et al, 2001;Shahzad et al, 2013;Sagervanshi et al, 2022), pea, spinach (Speer and Kaiser, 1991), and sunflower (Nikolic and Römheld, 2003), while up to about 19 mM K + was reported for common bean (O'Leary et al, 2016). In comparison, the AWFs gained from V. faba using the new method had at least 65% lower K + concentration compared to the traditional method (i.e.…”

Section: A New Opportunity For the Study Of The Leaf Apoplast Arises ...mentioning

confidence: 99%

“…These include insights into abiotic stress responses (Shahzad et al, 2013;Poschenrieder et al, 2008;Sagervanshi et al, 2022) plant-pathogen interactions, e.g. in terms of metabolite availability (Yamada et al, 2016;O'Leary et al, 2016), activation of plant defense responses (Wang et al, 2021) or recruitment of antimicrobial substances via extracellular vesicles (Rutter and Innes, 2017).…”

Section: Introductionmentioning

confidence: 99%

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In plantaexploitation of leaf apoplastic compounds: a window of opportunity for spatiotemporal studies of apoplastic metabolites, hormones and physiology

Franzisky

Sölter

Xue

et al. 2023

Preprint

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Processes in the leaf apoplast are relevant for development, cell wall rheological properties, plant nutrition, sink-source portioning, microbe-host plant-interactions or intercellular information exchange and signaling and are therefore regulated or influenced by the composition of the leaf apoplastic solute. In contrast to the traditional methods for the extraction of apoplastic solutes that are more or less destructive, we propose a new method that allows extraction of leaf apoplastic solutes (i) non-invasively and, thus, (ii) over time. Moreover, the method has (iii) a high spatial resolution that allows identification of solute-microdomains in the leaf apoplast. The method was established for Arabidopsis thaliana and Vicia faba leaves but should also be applicable to other plants species with similar leaf morphologies. It is based on the infiltration of an aqueous extraction solution into the apoplast followed by its recovery seconds later, both through the stomata. By this, the apoplast (and its solutes) of an identical leaf can be sampled on successive days with negligible symplastic contamination. A spatiotemporal mapping of leaf apoplastic ion and metabolite patterns within the identical leaf opens a window of opportunity for understanding apoplast biology. As for example, the existence of apoplastic abscisic acid gradients within a leaf in response to salinity was witnessed in this study, as was the unsuspected accumulation of kaempferol glycosides in the leaf apoplast. The presented method is relevant for plant developmental biologists, phytopathologists, plant physiologists, plant nutritionists and others that need to integrate apoplast biology into their research approaches.

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Section: /41mentioning

confidence: 63%

Section: Non-destructively Sampled Awfs Show Minimal Symplastic Conta...mentioning

confidence: 99%

Section: A New Opportunity For the Study Of The Leaf Apoplast Arises ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In plantaexploitation of leaf apoplastic compounds: a window of opportunity for spatiotemporal studies of apoplastic metabolites, hormones and physiology

Franzisky

Sölter

Xue

et al. 2023

Preprint

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“…Soil salinization is a severe environmental problem affecting global agriculture. The global area of saline-alkali soil is about 831 million hectares, of which 47% (about 397 million hectares) are saline soil and 53% (about 434 million hectares) are alkaline soil [ 1 ]. However, alkali stress with its high pH values is particularly detrimental to plants as compared with salt stress [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Physiological and Metabolic Responses of Leymus chinensis Seedlings to Alkali Stress

Yan

Shi

Chen

et al. 2022

Plants

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To elucidate the physiological and metabolic mechanism of perennial grass responses to alkali stress, we selected Leymus chinensis (L. chinensis), a salt-tolerant perennial rhizomatous species of the family Poaceae as experimental material. We conducted a pot experiment in a greenhouse and measured the biomass, physiological characteristics, metabonomic, and corresponding metabolites. Our results showed that alkali stress significantly inhibited seedling growth and photosynthesis, which caused ion imbalance and carbon deficiency, but the alkali stress significantly increased the nitrogen and ATP contents. The metabolic analysis indicated that alkali stress markedly enhanced the contents of nucleotides, amino acids, and organic acids, but it decreased soluble sugar contents. Pathway enrichment analysis showed that the glutamine synthetase/glutamate synthase (GS/GOGAT) cycle, which was related to nitrogen metabolism, was most significantly affected by alkali stress. The contents of glutamine synthetase (GS) and glutamate synthetase (GOGAT) involved in this pathway were also significantly increased. Our results not only verified the important roles of some amino acids and organic acids in resisting alkali stress, but also further proved that nucleotides and the GS/GOGAT cycle related to nitrogen metabolism played critical roles for seedlings in response to alkali stress.

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Sodium Bicarbonate Tolerance During Seedling Stages of Maize (Zea mays L.) Lines

Tian,

Ding,

Zhang

et al. 2024

Food and Energy Security

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ABSTRACT(1) Soil alkalinization and salinization represent a growing global challenge. Maize (Zea mays L.), with its relatively low tolerance to salt and alkali, is increasingly vulnerable to saline‐alkali stress. Identifying maize genotypes that can withstand salinity and alkalinity is crucial to broaden the base of salt‐alkali‐tolerant maize germplasm. (2) In this study, we screened 65 maize germplasm resources for alkali stress using a 60 mM NaHCO3 solution. We measured fifteen morphological and physiological indices, including seedling height, stem thickness, and leaf area. Various analytical methods—correlation analysis, principal component analysis, subordinate function analysis, cluster analysis, stepwise discriminant analysis, and ridge regression analysis—were used to assess the seedling alkali tolerance of these maize germplasm resources. The physiological indices of six tested maize varieties were analyzed in greater detail. (3) The findings revealed complex correlations among traits, particularly strong negative associations between conductivity and root traits such as length, volume, surface area, diameter, and number of branches. The 15 evaluation indices were reduced to 7 principal components, explaining 77.89% of the variance. By applying affiliation functions and weights, we derived a comprehensive evaluation of maize seedling alkali tolerance. Notably, three germplasms—Liang Yu 99, Bi Xiang 638, and Gan Xin 2818—demonstrated significant comprehensive seedling alkali tolerance. Cluster analysis grouped the 65 maize germplasm resources into four distinct categories (I, II, III, and IV). The results of the cluster analysis were confirmed by multiclass stepwise discriminant analysis, which achieved a correct classification rate of 92.3% for 60 maize genotypes regarding alkalinity tolerance. Using principal component and ridge regression analyses, we formulated a regression equation for alkali tolerance: D‐value = −1.369 + 0.002 * relative root volume + 0.003 * relative number of root forks + 0.006 * relative chlorophyll SPAD + 0.005 * relative stem thickness + 0.005 * relative plant height + 0.001 * relative conductivity + 0.002 * relative dry weight of underground parts. Under sodium bicarbonate stress, morphological indices and germination rates were significantly reduced, germination was inhibited, photosynthetic pigment levels in maize leaves decreased to varying degrees, and the activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) significantly increased. Alkali stress markedly enhanced the antioxidant enzyme activities in maize varieties, with alkali‐resistant varieties exhibiting a greater increase in antioxidant enzyme activities than alkali‐sensitive varieties under such stress. (4) By screening for alkali tolerance in maize seedlings, the identified alkali‐tolerant genotypes can be further utilized as suitable donor parents, thereby enhancing the use of alkali‐tolerant germplasm resources and providing theoretical guidance for maize cultivation in saline‐alkaline environments.

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Alkali salt stress causes fast leaf apoplastic alkalinization together with shifts in ion and metabolite composition and transcription of key genes during the early adaptive response of Vicia faba L

Cited by 7 publications

References 69 publications

In plantaexploitation of leaf apoplastic compounds: a window of opportunity for spatiotemporal studies of apoplastic metabolites, hormones and physiology

In plantaexploitation of leaf apoplastic compounds: a window of opportunity for spatiotemporal studies of apoplastic metabolites, hormones and physiology

Physiological and Metabolic Responses of Leymus chinensis Seedlings to Alkali Stress

Sodium Bicarbonate Tolerance During Seedling Stages of Maize (Zea mays L.) Lines

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