Alkaline and acid phosphatases of the silkworm, Bombyx mori L.

Sridhara, S.; Bhat, Jayashree S.

doi:10.1016/0022-1910(63)90012-x

Cited by 58 publications

(33 citation statements)

References 17 publications

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“…To establish a method for isolation of E. mundtii from mulberry leaves phylosphere, which may contain multiple microbial florae other than E. mundtii, we attempted to find growth conditions specific for E. mundtii. Since we have demonstrated that E. mundtii proliferated in intestine of silkworm under alkaline conditions, and corroborated by previous reports (19,20), we hypothesized that E. mundtii was resistant to alkaline conditions. To test this, we compared the growth of E. mundtii and Staphylococcus aureus or Pseudomonas aeruginosa (common environmental bacteria) on agar BHI plates with various concentrations of Sodium Hydroxide (NaOH).…”

Section: E Mundtii Isolation From Mulberry Leavessupporting

confidence: 67%

Identification and methods for prevention of Enterococcus mundtii infection in silkworm larvae, Bombyx mori, reared on artificial diet

Nwibo

Matsumoto

Sekimizu

2015

DD&T

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Section: E Mundtii Isolation From Mulberry Leavessupporting

confidence: 67%

Identification and methods for prevention of Enterococcus mundtii infection in silkworm larvae, Bombyx mori, reared on artificial diet

Nwibo

Matsumoto

Sekimizu

2015

DD&T

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“…The decrease in the activity of acid and alkaline phosphatases leads to increment in acid soluble phosphorus (Sridhara and Bhat, 1963). Additionally, Zaidi et al (1985) reported that the elevation of phosphorus level might be due to the inhibition of certain enzymes because of necrosis or lysis of certain body structures after infection.…”

Section: Changes In Nitrogen (N) and Phosphorus (P) Contentsmentioning

confidence: 99%

Metabolic profiles of pathogen-challenged mulberry silkworm, Bombyx mori L. as a tool for disease diagnosis

Taha¹,

Ismail²,

Abdel-Gawad³

2018

Biochem Biotechnol Res

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Metabolic profiles were detected in healthy and flacherie (inoculated with Bacillus sp.) and grasserie (inoculated with polyhedral bodies)-diseased Bombyx mori larvae. Free amino acids were separated and quantitatively determined by using hydrolysis method. Further, nitrogen (N) percentage was estimated with micro-Kieldahl method. Whereas, phosphor-molybdate method was applied for phosphorus (P) analysis. Results revealed that distinct differences in the amounts of eighteen individual amino acids in healthy and diseased silkworm 5 th larval instar were found. The highest amino acids concentration was recorded in healthy larvae (224.05 mg/g tissue), while the lowest concentration was found in Gr-diseased larvae (177.77 mg/g tissue). Fl-diseased larvae inoculated with Bacillus sp. showed elevation in certain amino acids comparing with Gr-diseased larvae inoculated with of Nuclear Polyhedrosis Virus (NPV) occluded bodies. N and P percentages in Fl and Gr diseases recorded (N = 2.57%, P = 0.29%), (N = 3.86%, P = 0.32%) respectively, compared with healthy larvae (N = 1.63%, P = 0.23%). The present study reveals that a decrease in amino acids in fifth-larval stage implies an increase in N and P percentages in both bacterial and viral infected larvae. It is concluded that the determination of biochemical responses in silkworm against infections may be considered as bio-analytical markers, which are useful for early disease diagnosis and developing disease resistant breeds.

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“…During the last two decades work on biochemical characterization of phosphatases in insects has been done by Drilhon and Busnel (1945), Fitzgerald (1949), Rockstein and Herron (1951), Rockstein and Levine (1951), Denuce (1952), Rockstein (1956), Barker and Alexander (1958), Lambremont (1959), Fisk (1961, 1962), Hodgson (1963), Sridhara and Bhat (1963), Huddleston (1965), McCaman, Smith, andCock (1965), Raychaudri and Butz (1965), Hirano and Gilbert (1967), and Naqvi, Ashrafi, and Qadri (1967).…”

Section: Introduotionmentioning

confidence: 99%

Acid Phosphatase Activity in the Digestive System of the Desert Locust Schistocerca Gregaria (Forskal)

Ashrafi¹,

Qadri²

1968

Aust. Jnl. Of Bio. Sci.

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SummaryAcid phosphatase activity was determined biochemically in a homogenate of the digestive tract of the desert locust, S. gregaria. p-Nitrophenol was used as colorimetric standard and disodium p.nitrophenyl phosphate as substrate.The enzyme was found to have a linear relationship between concentration and activity, maximum activity at pH 4· 4, a Michaelis constant of 3·0 X 10-4M, and a temperature coefficient (QlO) of 2 ·10 between 20 and 30°C. Zero-order kinetics were maintained for 35 min at 40°C. Magnesium, calcium, and potassium ions and magnesium and phenylmercuric acetates slightly activated the enzyme, while ferrous ion, sodium fluoride, disodium hydrogen phosphate, and disodium hydrogen arsenate inhibited the enzyme. The magnesium ion concentration required for maximum activation was found to be O· 025M. INTRODUOTIONPhosphomonoesterases or phosphoric monoester hydrolases (3.l.3, Thompson 1962) are widely present in animal and plant tissues. During the last two decades work on biochemical characterization of phosphatases in insects has been done by Drilhon and Busnel (1945), Fitzgerald (1949), Rockstein and Herron (1951), Rockstein and Levine (1951), Denuce (1952), Rockstein (1956), Barker and Alexander (1958), Lambremont (1959), Fisk (1961, 1962), Hodgson (1963), Sridhara and Bhat (1963), Huddleston (1965), McCaman, Smith, andCock (1965), Raychaudri and Butz (1965), Hirano and Gilbert (1967), and Naqvi, Ashrafi, and Qadri (1967).In general these authors have studied the phosphomonoesterases in homogenates of holometabolous insects, whereas the present work deals with the biochemical characterization of acid phosphatase in the alimentary canal of a hemimetabolous insect, the desert locust, Schistocerca gregaria (Forskal). II. MATERIALS AND METHODS Enzyme SourceAdult locusts (28 days old) which had been fed on 6% glucose solution for 1 day prior to the experiment were selected as the source of enzymes. Locusts were dissected in cold doubledistilled demineralized water and the alimentary canal was transferred to a test tube containing 5 ml chilled double-distilled demineralized water. It was ground for exactly 3 min in a TeflonPyrex tissue grinder. The homogenate was filtered through a 2-mm layer of glass fibre in a

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Alkaline and acid phosphatases of the silkworm, Bombyx mori L.

Cited by 58 publications

References 17 publications

Identification and methods for prevention of <i>Enterococcus mundtii</i> infection in silkworm larvae, <i>Bombyx mori</i>, reared on artificial diet

Identification and methods for prevention of <i>Enterococcus mundtii</i> infection in silkworm larvae, <i>Bombyx mori</i>, reared on artificial diet

Metabolic profiles of pathogen-challenged mulberry silkworm, Bombyx mori L. as a tool for disease diagnosis

Acid Phosphatase Activity in the Digestive System of the Desert Locust Schistocerca Gregaria (Forskal)

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