Alkaline Comet Assay for Genotoxic Effect Detection in Neotropical Fish Prochilodus lineatus (Pisces, Curimatidae)

Simoniello, María Fernanda; Gigena, F.; Poletta, Gisela Laura; Loteste, Alicia Elena; Kleinsorge, E. C.; Campana, Mirta; Scagnetti, J.; Parma, Maria Julieta E.

doi:10.1007/s00128-009-9771-z

Cited by 40 publications

(37 citation statements)

References 13 publications

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“…Their relationship may be useful as integrated environmental diagnostic tools and in developing control strategies and pollution prevention measures (Pathiratne et al 2008). The use of fish in freshwater and marine environments as bioindicators of environmental pollution has become more common in aquatic monitoring programs since the practice has proved useful in identifying environmental problems, mainly on the evaluation of the presence of potentially teratogenic and carcinogenic toxins (Simoniello et al 2009). Genotoxicity and endocrine disruption are the most worrying effects for their ecological implications in aquatic ecosystems.…”

Section: Introductionmentioning

confidence: 99%

Exposure to persistent organic pollutants (POPs) and DNA damage as an indicator of environmental stress in fish of different feeding habits of Coatzacoalcos, Veracruz, Mexico

González-Mille

Ilizaliturri-Hernández

Espinosa-Reyes

et al. 2010

Ecotoxicology

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The region of Coatzacoalcos, Veracruz hosts one of the largest and most important industrial areas of Mexico and Latin America. Industrial development and rapid population growth, have triggered a severe impact on aquatic ecosystems of the region. The aim of this study was to determine the levels of POPs in sediment and in muscle tissue of five fish species from different trophic levels in downstream residents of the Coatzacoalcos River, and their integration with DNA damage in the fish, evaluated with the comet assay in whole blood as a biological indicator of stress, in order to obtain a baseline of the ecological condition of the region. The compounds detected in sediment and in muscle tissue were hexachlorobenzene (HCB), α-, β-, γ-hexachlorocyclohexane (HCH), dichlorodiphenyltrichloroethane (DDT), dichlorodiphenyldichloroethylene (DDE), mirex and polychlorinated biphenyls (PCBs). Sediment concentrations of these pollutants (except for mirex) exceeded the values of protection provided by international guidelines, suggesting a potential risk to aquatic life in the region. DNA damage recorded in the fish species is evidence of exposure to a mix of genotoxic pollutants, which combined with exposure to POPs, reflects the degree of environmental stress of aquatic organisms in the region. The results of this study show the importance of determining the presence of contaminants in the environment, the bioaccumulation in tissues and their effects on exposed organisms, providing an integrated approach in assessing the health of aquatic ecosystems.

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Section: Introductionmentioning

confidence: 99%

Exposure to persistent organic pollutants (POPs) and DNA damage as an indicator of environmental stress in fish of different feeding habits of Coatzacoalcos, Veracruz, Mexico

González-Mille

Ilizaliturri-Hernández

Espinosa-Reyes

et al. 2010

Ecotoxicology

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“…Results obtained by Campana et al (1999) demonstrated the genotoxic effects of lambda-cyhalothrin on erythrocytes of Cheirodon interruptus interruptus. Simoniello et al (2009) reported that cypermethrin showed a signifi cantly higher level of DNA damage in the fi sh species Prochilodus lineatus after in vivo exposure to different concentrations of cypermethrin.…”

Section: Resultsmentioning

confidence: 99%

“…Information on the genotoxic effects of pyrethroid insecticides on fi sh species is limited. However, some studies have demonstrated that synthetic pyrethroids are genotoxic to fi sh (Campana et al, 1999;Çavas and Ergene-Gözükara, 2003;Simoniello et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

In vivo Genotoxicity of the Pyrethroid Pesticide β-Cyfluthrin Using the Comet Assay in the Fish Bryconamericus iheringii

Marinowic

Mergener

Pollo

et al. 2012

Zeitschrift Für Naturforschung C

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Environmental pollution by pesticide residues is a major environmental concern due to the extensive use of these substances in agriculture. The insecticide β-cyfl uthrin is a synthetic pyrethroid widely used in agricultural and other domestic activities. The aim of the present study was to assess the genotoxic effects of a sublethal exposure of the fi sh Bryconamericus iheringii (Characidae) to a commercial formulation of β-cyfl uthrin using the comet assay. Fish were exposed to sublethal concentrations (4.2 and 5.6 µg/L) of β-cyfl uthrin under static conditions during 24-and 48-h exposure periods. Fish in tap water were used as negative controls. Results obtained by the comet assay revealed genotoxic effects of the pyrethroid in the higher concentration and at the longer exposure period. The mean DNA damage index of fi sh exposed to 5.6 µg/L β-cyfl uthrin for 48 h was signifi cantly higher (145.9  51.8) than in the control group (69.3  39.5). These fi ndings indicate that native fi sh species might be at risk for genotoxic damage in waters contaminated with β-cyfl uthrin.

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“…Disturbances in the DNA molecule such as adducts, single, and double-strand breaks may cause permanent damage that induces MN, being then an excellent biomarker to evaluate any permanent DNA damage (Vanzella et al, 2007). Increased MN frequencies in blood cells of the Neotropical fish Prochilodus lineatus exposed to diesel water soluble fraction under acute and subchronic conditions, demonstrated that MN is an efficient endpoint for detecting mutagenic compounds in water (Simoniello et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Lack of genotoxicity in Astyanax bimaculatus and Oreochromis niloticus of 17α-methyltestosterone used in fish hatcheries to produce male monosex populations

Rivero-Wendt

Miranda-Vilela²,

Ferreira³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. 17α-Methyltestosterone (MT) is widely used in fish hatcheries of many countries to produce male monosex populations. Its genotoxic risk to fish species is not well known and studies in other in vivo models are still inconclusive. MT was tested for genotoxicity in the fish species Oreochromis niloticus (tilapia), a target species, and Astyanax bimaculatus (lambari), a native non-target species. Genotoxicity was evaluated by the micronucleus test (MN), nuclear abnormalities (NA), and comet assay using peripheral erythrocytes of both species after a 96-h exposure to MT at concentrations of 0.01, 0.1, and 1.0 mg/L in the water. At the lowest exposure level of 0.01 These effects were not observed in the comet assay. Chromatographic analysis of water samples collected from aquariums at the beginning and end of each experiment showed that MT was consumed during the 96-h exposure. At the highest level of exposure (1.0 mg/L), 81.69% of the hormone was consumed during the exposure period. The chromatogram showed that at the lowest concentration level of 0.01 mg/L, 99.56% MT was consumed by the end of the exposure period. Thus, exposure to MT did not cause genotoxicity in either fish species.

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Alkaline Comet Assay for Genotoxic Effect Detection in Neotropical Fish Prochilodus lineatus (Pisces, Curimatidae)

Cited by 40 publications

References 13 publications

Exposure to persistent organic pollutants (POPs) and DNA damage as an indicator of environmental stress in fish of different feeding habits of Coatzacoalcos, Veracruz, Mexico

Exposure to persistent organic pollutants (POPs) and DNA damage as an indicator of environmental stress in fish of different feeding habits of Coatzacoalcos, Veracruz, Mexico

In vivo Genotoxicity of the Pyrethroid Pesticide β-Cyfluthrin Using the Comet Assay in the Fish Bryconamericus iheringii

Lack of genotoxicity in Astyanax bimaculatus and Oreochromis niloticus of 17α-methyltestosterone used in fish hatcheries to produce male monosex populations

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