Alkaline pH shock enhanced production of validamycin A in fermentation of Streptomyces hygroscopicus

Jiang, Jing; Sun, Ya-Fang; Tang, Xi; He, Chaonan; Shao, Yang; Tang, Ya‐Jie; Zhou, Wen-Wen

doi:10.1016/j.biortech.2017.10.012

Cited by 30 publications

(15 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fluorescence intensities were measured with a fluorescence spectrophotometer (Hitachi F-7000, Japan) at an excitation wavelength of 488 nm and an emission wavelength of 535 nm. The pHi calibration was performed with 10.0 μM nigericin (Sigma-Aldrich) and a set of high K + phosphate buffer (6.0–8.0). − Three biological repeats were performed for pH measurement.…”

Section: Methodsmentioning

confidence: 99%

Proteomic Investigation of the Antibacterial Mechanism of trans-Cinnamaldehyde against Escherichia coli

Yin

Yang

et al. 2021

J. Proteome Res.

View full text Add to dashboard Cite

Trans-Cinnamaldehyde (TC) is a widely used food additive, known for its sterilization, disinfection, and antiseptic properties. However, its antibacterial mechanism is not completely understood. In this study, quantitative proteomics was performed to investigate differentially expressed proteins (DEPs) in Escherichia coli in response to TC treatment. Bioinformatics analysis suggested aldehyde toxicity, acid stress, oxidative stress, interference of carbohydrate metabolism, energy metabolism, and protein translation as the bactericidal mechanism. E. coli BW25113ΔyqhD, ΔgldA, ΔbetB, ΔtktB, ΔgadA, ΔgadB, ΔgadC, and Δrmf were used to investigate the functions of DEPs through biochemical methods. The present study revealed that TC exerts its antibacterial effects by inducing the toxicity of its aldehyde group producing acid stress. These findings will contribute to the application of TC in the antibacterial field.

show abstract

Section: Methodsmentioning

confidence: 99%

Proteomic Investigation of the Antibacterial Mechanism of trans-Cinnamaldehyde against Escherichia coli

Yin

Yang

et al. 2021

J. Proteome Res.

View full text Add to dashboard Cite

show abstract

“…In addition to the carbon source, which plays a direct role in pseudo-oligosaccharide biosynthesis, nitrogen concentration (59), temperature (60, 61), alkaline pH shock (62), and intracellular reactive oxygen species (ROS) (63–65) can affect validamycin production in S. hygroscopicus 5008. In contrast to the normal growth and sporulation temperature of actinomycetes, the optimal temperature for validamycin A production is 35-37 °C.…”

Section: Effects Of Medium and Growth Condition On Pseudo-oligosacchamentioning

confidence: 99%

“…A sudden change of pH can also increase validamycin production. An alkaline pH shock using an NaOH solution (pH 8) at 20 h of incubation was found to increase validamycin A production by 27% (62). This change in pH also increased the sugar consumption rate and the cell weight after 96 h of incubation.…”

Section: Effects Of Medium and Growth Condition On Pseudo-oligosacchamentioning

confidence: 99%

Biosynthesis and metabolic engineering of pseudo-oligosaccharides

Alanzi

Demessie

Mahmud

2018

Emerging Topics in Life Sciences

View full text Add to dashboard Cite

Pseudo-oligosaccharides are microbial-derived secondary metabolites whose chemical structures contain pseudosugars (glycomimetics). Due to their high resemblance to the molecules of life (carbohydrates), most pseudo-oligosaccharides show significant biological activities. Some of them have been used as drugs to treat human and plant diseases. Because of their significant economic value, efforts have been put into understanding their biosynthesis, optimizing their fermentation conditions, and engineering their metabolic pathways to obtain better production yields. A number of unusual enzymes participating in diverse biosynthetic pathways to pseudo-oligosaccharides have been reported. Various methods and conditions to improve the production yields of the target compounds and eliminate byproducts have also been developed. This review article describes recent studies on the biosynthesis, fermentation optimization, and metabolic engineering of high-value pseudo-oligosaccharides.

show abstract

“…Similarly, pH serves as a reference to monitor and prevent corrosion in pipes and boilers [4,5]. In fermentation processes, continuous quantification of the pH is relevant to avoid the generation of unwanted and harmful by-products [6][7][8]. In the brewing industry, the pH serves to determine aging, increments in hop hardness, and bittering concentrations [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Cost-Effective and Portable Instrumentation to Enable Accurate pH Measurements for Global Industry 4.0 and Vertical Farming Applications

et al. 2022

View full text Add to dashboard Cite

Global Vertical Farming (VF) applications with characteristic Industry 4.0 connectivity will become more and more relevant as the challenges of food supply continue to increase worldwide. In this work, a cost-effective and portable instrument that enables accurate pH measurements for VF applications is presented. We demonstrate that by performing a well-designed calibration of the sensor, a near Nernstian response, 57.56 [mV/pH], ensues. The system is compared to a ten-fold more expensive laboratory gold standard, and is shown to be accurate in determining the pH of substances in the 2–14 range. The instrument yields precise pH results with an average absolute deviation of 0.06 pH units and a standard deviation of 0.03 pH units. The performance of the instrument is ADC-limited, with a minimum detectable value of 0.028 pH units, and a typical absolute accuracy of ±0.062 pH units. By meticulously designing bias and amplification circuitry of the signal conditioning stage, and by optimizing the signal acquisition section of the instrument, a (minimum) four-fold improvement in performance is expected.

show abstract

Alkaline pH shock enhanced production of validamycin A in fermentation of Streptomyces hygroscopicus

Cited by 30 publications

References 29 publications

Proteomic Investigation of the Antibacterial Mechanism of trans-Cinnamaldehyde against Escherichia coli

Proteomic Investigation of the Antibacterial Mechanism of trans-Cinnamaldehyde against Escherichia coli

Biosynthesis and metabolic engineering of pseudo-oligosaccharides

Cost-Effective and Portable Instrumentation to Enable Accurate pH Measurements for Global Industry 4.0 and Vertical Farming Applications

Contact Info

Product

Resources

About