Alkaline phosphatase activities of cultured human periodontal ligament cells.

Ohshima, Mitsuhiro; Kuwata, Fumiyuki; Otsuka, Kichibee; Saito, Ryozo; Sato, Kenzo; Shioji, Shogo; Suzuki, Kantaro

doi:10.2334/josnusd1959.30.208

Cited by 33 publications

(18 citation statements)

References 9 publications

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“…Many investigators have reported that the ALPase activities of these cells increase at an initial differentiation stage and that they are suitable for one of the initial differentiation markers. 17 On the other hand, using DMEM-A, HPLF did not construct aggregates on CPEC at 4 days, and ALPase activity did not increase. In order to increase ALPase activity on CPEC, it is necessary that HPLF forms aggregate and contact each other.…”

Section: Discussionmentioning

confidence: 99%

“…Inside HPLF, aggregate cells contacted each other in a manner similar to contact inhibition conditions in confluence. In general, the ALPase activities of HPLF and osteoblasts are increased when these cells grow to confluence 17,18 and, at that time, cell proliferation is stopped and the cells enter a differentiation phase. Many investigators have reported that the ALPase activities of these cells increase at an initial differentiation stage and that they are suitable for one of the initial differentiation markers.…”

Section: Discussionmentioning

confidence: 99%

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Effects of polyelectrolyte complex (PEC) on human periodontal ligament fibroblast (HPLF) function. II. Enhancement of HPLF differentiation and aggregation on PEC by L-ascorbic acid and dexamethasone

Hamano

Teramoto

Iizuka

et al. 1998

J. Biomed. Mater. Res.

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In addition to many types of extra cellular matrix (ECM) in vivo, cells are stimulated by many types of vitamins, hormones, growth factors, etc. In this paper the effects of L-ascorbic acid 2-phosphate (Asc-2P) and dexamethasone (Dex) on proliferation and differentiation of human periodontal ligament fibroblast (HPLF) using polyelectrolyte complex (PEC) as a matrix in vitro will be discussed. The PEC was composed of chitosan as a polycation, with carboxymethyl (CPEC) or sulfated chitin (SPEC). Asc-2P (0.2 mM) inhibited the growth of HPLF on CPEC, but promoted the growth on SPEC. Moreover, the aggregation of HPLF on CPEC was inhibited by Asc-2P, but that on SPEC was induced in the presence of Asc-2P and Dex. Although Asc-2P reduced an increase in alkaline phosphatase (ALPase) activity of HPLF on CPEC as well, it induced a twofold increase in ALPase activities on SPEC and TCD. Furthermore, in the medium containing Asc-2P and 100 mM of Dex, cell growth was inhibited, but ALPase activity was promoted on both SPEC and TCD to form many aggregates on SPEC. ALPase activity increased by twofold over that of HPLF cultured in the medium containing only Asc-2P. Therefore, it is suggested that the cell functions of HPLF are controlled by the combination of PEC and additives.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of polyelectrolyte complex (PEC) on human periodontal ligament fibroblast (HPLF) function. II. Enhancement of HPLF differentiation and aggregation on PEC by L-ascorbic acid and dexamethasone

Hamano

Teramoto

Iizuka

et al. 1998

J. Biomed. Mater. Res.

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“…Recognized therapeutic strategies, such as guided tissue regeneration (Caton et al 1992) and coronally positioned flaps designed to exclude epithelial cells from regenerating areas (Martin et al 1988), provide indirect evidence for the importance of mesenchymal cells in this process (PDL fibroblasts, cementoblasts, or osteoblasts). Since, PDL fibroblasts express alkaline phosphatase (Groeneveld et al 1995;Yamaguchi et al 1996), a marker for developing bone cells that is not expressed by gingival fibroblasts (Giannopoulou and Cimasoni 1996;Ohshima et al 1988), the source for the cells that repair bone may overlap those that repair the ligament. Several studies have presented evidence that PDL fibroblasts are capable of differentiating into osteoblasts (Bernick et al 1989;Reddi 1981;Urist et al 1983), are chemotactically responsive to appropriate growth factors (Nishimura and Terranova 1996), and participate in defect repair at surgical sites (Narayanan and Page 1983a,b).…”

Section: Introductionmentioning

confidence: 98%

A simple cell motility assay demonstrates differential motility of human periodontal ligament fibroblasts, gingival fibroblasts, and pre-osteoblasts

et al. 2007

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During periodontal regeneration, multiple cell types can invade the wound site, thereby leading to repair. Cell motility requires interactions mediated by integrin receptors for the extracellular matrix (ECM), which might be useful in guiding specific cell populations into the periodontal defect. Our data demonstrate that fibroblasts exhibit differential motility when grown on ECM proteins. Specifically, gingival fibroblasts are twice as motile as periodontal ligament fibroblasts, whereas osteoblasts are essentially non-motile. Collagens promote the greatest motility of gingival fibroblasts in the following order: collagen III>collagen V>collagen I. Differences in motility do not correlate with cell proliferation or integrin expression. Osteoblasts display greater attachment to collagens than does either fibroblast population, but lower motility. Gingival fibroblast motility on collagen I is generally mediated by alpha2 integrins, whereas motility on collagen III involves alpha1 integrins. Other integrins (alpha10 or alpha11) may also contribute to gingival fibroblast motility. Thus, ECM proteins do indeed differentially promote the cell motility of periodontal cells. Because of their greater motility, gingival fibroblasts have more of a potential to invade periodontal wound sites and to contribute to regeneration. This finding may explain the formation of disorganized connective tissue masses rather than the occurrence of the true regeneration of the periodontium.

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“…Recognized therapeutic strategies, such as guided tissue regeneration (Caton et al 1992) and coronally positioned flaps designed to exclude epithelial cells from regenerating areas (Martin et al 1988), provide indirect evidence for the importance of mesenchymal cells in this process (PDL fibroblasts, cementoblasts, or osteoblasts). Since PDL fibroblasts express alkaline phosphatase (Groeneveld et al 1995;Yamaguchi et al 1996), a marker for developing bone cells not expressed by gingival fibroblasts (Giannopoulou and Cimasoni 1996;Ohshima et al 1988), the source for the cells that repair the ligament may overlap those that repair bone. Several studies have presented evidence that PDL fibroblasts are capable of differentiating into osteoblasts (Bernick et al 1989;Reddi 1981;Urist et al 1983), are chemotactically responsive to appropriate growth factors (Nishimura and Terranova 1996), and participate in defect repair at surgical sites (Narayanan and Page 1983).…”

Section: Introductionmentioning

confidence: 98%

Use of microarrays to find novel regulators of periodontal ligament fibroblast differentiation

Lallier

Spencer

2006

Cell Tissue Res

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Periodontal regeneration requires the coordinated movement and differentiation of several cell types in order to re-establish the cementum, periodontal ligament (PDL), and alveolar bone. Cells in culture are often used as model systems for mature tissues, although they may represent expanded progenitor cell populations. Comparison of transcript expression between fresh PDL tissue and PDL cell isolates by MicroArray analysis has revealed numerous molecular differences. Several transcripts (including alkaline phosphatase, bone sialoprotein, periostin, and fibromodulin) are expressed at higher levels in fresh PDL than in cultured PDL cells. In contrast, PDL cells in culture selectively express a variety of growth factors. Several of these growth factors alter PDL fibroblast behavior. Two members of the transforming growth factor beta family of growth factors, namely, bone morphogenic protein-7 (BMP7) and growth differentiation factor-5 (GDF5), reduce cell proliferation and Stro-1 expression (a bone marrow stromal stem cell marker), whereas only BMP7 induces alkaline phosphatase activity. In contrast, fibroblast growth factor-5 induces enhanced cell proliferation and Stro-1 expression, while repressing alkaline phosphatase activity. The stimulation of PDL cells to differentiate (either by BMP7 or GDF5) inhibits cell motility. Thus, PDL cells in culture are regulated by several factors that differentially stimulate a mineralized (cementoblast-like) fate, a non-mineralized fate (mature fibroblasts), or the propagation of a more naive phenotype (potential progenitors).

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Alkaline phosphatase activities of cultured human periodontal ligament cells.

Cited by 33 publications

References 9 publications

Effects of polyelectrolyte complex (PEC) on human periodontal ligament fibroblast (HPLF) function. II. Enhancement of HPLF differentiation and aggregation on PEC by L-ascorbic acid and dexamethasone

Effects of polyelectrolyte complex (PEC) on human periodontal ligament fibroblast (HPLF) function. II. Enhancement of HPLF differentiation and aggregation on PEC by L-ascorbic acid and dexamethasone

A simple cell motility assay demonstrates differential motility of human periodontal ligament fibroblasts, gingival fibroblasts, and pre-osteoblasts

Use of microarrays to find novel regulators of periodontal ligament fibroblast differentiation

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