ALKALINE PHOSPHATASE AND Β-Glucuronidase ACTIVITY IN THE RAT UTERUS DURING EARLY PREGNANCY

Manning, John P.; Meli, A; Steinetz, Bernard G.

doi:10.1677/joe.0.0350385

Cited by 26 publications

(12 citation statements)

References 15 publications

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“…Although there was about a twofold increase in the activity of this enzyme at the implantation sites between days 7 and 8, the main increase took place in the decidual nodules confirming previous findings (18,19,27) of a 10-fold increase in alkaline phosphatase activity on day 9 of pregnancy in the rat uterus, an increase localized exclusively to the implantation sites presumably containing the decidua. The biochemical findings also support the histochemical demonstra tions of increased alkaline phosphatase activity associated with decidualization (3,15,23).…”

Section: Discussionsupporting

confidence: 89%

Enzymic Activity in Rat Uterus during Early Pregnancy

Malinowska¹,

Fotherby²

1975

Horm Res

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Total protein, RNA and DNA content and the activity of acid and alkaline phosphatases, 5´-nucleotidase and isocitrate dehydrogenase were studied in rat uterus during the first 8 days of pregnancy. Isocitrate dehydrogenase activity showed marked fluctuations from day to day. Nucleotidase and acid phosphatase activities showed a significant increase on day 8. The most marked change in activity was that of alkaline phosphatase which showed a 10-fold increase between days 6 and 8, due largely to an increase in the activity of this enzyme in the decidual nodule. The rise in alkaline phosphatase activity did not occur in rats ovariectomized on days 1, 2 or 4 of pregnancy and was markedly decreased in those ovariectomized on day 6. [³H]-uridine incorporation into RNA showed a significant increase between days 2 and 6 whereas [³H]-thymidine incorporation into DNA showed a significant increase on day 6.

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Section: Discussionsupporting

confidence: 89%

Enzymic Activity in Rat Uterus during Early Pregnancy

Malinowska¹,

Fotherby²

1975

Horm Res

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“…A recent paper compares changes in the pregnant and corresponding sterile horns of the rat up to 8 days p.c. (Manning, Meli & Steinetz, 1966). The authors conclude that there is evidence of enzymic activity which is entirely dependent upon the interaction between the blasto¬ cyst and the hormonally conditioned uterus, and not upon hormonal stimulation alone.…”

Section: Discussionmentioning

confidence: 92%

The Role of the Fertilized Egg: Reactions in the Guinea-Pig Uterus at Ovoimplantation and After Thread Traumatization

Deanesly¹

1967

Reproduction

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Unilaterally sterilized guinea-pigs were mated and the sterile uterine horn traumatized 0 to 7 days later by the passage of two or more threads which were left in situ. The animals were killed 3 to 7 days after traumatization and the endometrial reactions studied histologically. Although deciduomata developed in uteri traumatized 4 to 7 days after mating, those traumatized up to 3 days after mating and killed up to 5 days later showed little reaction and contrasted markedly with the corresponding horns in which fertilized eggs were becoming implanted. This difference, not affected by exogenous progesterone treatment, indicates that the fertilized eggs have a specific capacity to induce changes in the endometrium, while it is non-reactive to other stimuli.Traumatization of both uterine horns did not completely inhibit ovoimplantation or subsequent pregnancy.

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“…The uterine environment for all stages of fetal development and survival to term is important. The activities of uterine acid and alkaline phosphatases associated with implantation (Finn & Hinchliffe, 1964;Manning, Meli & Steinetz, 1966;Murdoch, 1970), embryo growth and survival, uterine carbohydrate metabolism (Fahmy, 1957) and glycogen deposition (Christie, 1966) (Ducommun, 1962) The animals were examined every 2 h for parturition. Immediately after parturition, the uteri were removed, placed on ice and quickly frozen to -20°C until homogenized with ice-cold physiological saline (9 g NaCl/1), centrifuged and the supernatant taken for assay of the activities of alkaline (EC 3.1.3.1) and acid (EC 3.1.3.2) phosphatases (King & King, 1954); glutamic oxaloacetic acid (GOT, EC 2.6.1.1) and glutamic pyruvic acid (GPT, EC 2.6.1.2) transaminases (Reitman & Frankel, 1957) and protein (Lowry, Rosebrough, Farr & Randall, 1951).…”

Section: Introductionmentioning

confidence: 99%

Uterine enzyme changes in thyroidectomized rats at parturition

Rao¹,

Panda²

1981

Reproduction

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Summary. Rats were thyroidectomized at different stages of gestation and killed at parturition. Acid phosphatase of uterine horns was significantly (P < 0\m=.\05)lower in animals thyroidectomized at later stages of pregnancy, whereas alkaline phosphatase was significantly lower in those thyroidectomized earlier as compared to intact pregnant animals. The activities of glutamic oxaloacetic acid and glutamic pyruvic acid transaminases also showed significant alterations in some of the thyroidectomized animals. These 4 enzymes were also studied in the cervix but the changes showed a less distinct pattern.

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ALKALINE PHOSPHATASE AND Β-Glucuronidase ACTIVITY IN THE RAT UTERUS DURING EARLY PREGNANCY

Cited by 26 publications

References 15 publications

Enzymic Activity in Rat Uterus during Early Pregnancy

Enzymic Activity in Rat Uterus during Early Pregnancy

The Role of the Fertilized Egg: Reactions in the Guinea-Pig Uterus at Ovoimplantation and After Thread Traumatization

Uterine enzyme changes in thyroidectomized rats at parturition

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