Alkaline phosphatase, cytokeratin 7, cytokeratin 8 in the diagnosis of primary lung adenocarcinoma from 148 pleura fluids specimens.

Abstract

Adenocarcinomas are the most common cause of malignancy in pleura fluids. Usual primary sites include the lung, breast, gastrointestinal tract, and genitourinary tracts. Predicting the site of origin of an adenocarcinoma can be difficult due to overlapping morphologic characteristics. We investigated the use of alkaline phosphatase (AP), Cytokeratin7 (CK7) Cytokeratin8 (CK8) to distinguish adenocarcinomas of lung in 148 body cavity fluid samples. Overall results for primary lung adenocarcinomas, demonstrated CK8 reactivity in 106 (72%) of 148 cases. 95 primary lung carcinoma samples (65%) were positive for CK7. AP was expressed in 81% of primary lung adenocarcinomas. Positive immunoreactivity for AP was characterized by a red, diffusely apical cytoplasmic staining in tumor cells that ocurred singly or in groups. There was a significant difference between AP, CK 7 and CK 8 expressions in primary lung adenocarcinomas (P=0.02; Chisquared test). The sensitivity of AP, CK8, CK7 as a marker for primary lung adenocarcinomas were 82%, 72%, 64%, respectively. Thus the AP positive staining largely confirmed the cytologic diagnosis of lung adenocarcinoma.

Detection of alkaline phosphatase in canine cells previously stained with Wright–Giemsa and its utility in differentiating osteosarcoma from other mesenchymal tumors

“…ALP activity has been noted in germ cell tumors, such as seminomas, non germ cell line tumors, such as pulmonary, ovarian, and uterine carcinomas, and tumors of myogenic origin. ALP activity has been shown to be highly specific for human primary adenocarcinomas of lung origin; thus, the expression of ALP by cells from a bronchoalveolar carcinoma in a dog could be an expected finding. The anaplastic sarcoma in our study that expressed ALP also expressed vimentin, but was not immunoreactive for CD18, Melan A, actin, desmin or cytokeratin; bone origin could not be ruled out, and reasons for ALP expression were speculative.…”

Newly Identified Biomarkers for Detecting Circulating Tumor Cells in Lung Adenocarcinoma

“…CK7, a member of the CK family, has been reported in breast cancer, renal carcinoma, bladder cancer, and 97.0%-100.0% of lung adenocarcinoma cases, including primary tissues, metastatic organs, and cavity liquid (Camilo et al 2006;Serpil et al 2009;Dukic et al 2011). Immuno histochemical examination of CK7 and CK20 has been used diagnostically for discrimination of primary lung adenocarcinoma and lung metastasis of colorectal cancer (Ikeda et al 2006).…”

Proteomic and ultrastructural analysis of Clara cell and type II alveolar epithelial cell-type lung cancer cells

“…Among them, UCHL1 was not verified by western blot, and CK19, CK8, and PRDX2 were clearly differentially expressed between the two cell lines; however, there was no strong-or-nothing expression pattern. Previous studies also have revealed high expression levels of CK19 and CK8 in almost all non-small cell lung cancer cells (16)(17)(18)(19)(20), so CK19 and CK8 appeared to be suitable as biomarkers for non-small cell lung cancer cells but not for Clara cell-type lung cancer cells and were excluded from further study. ERO1L was uniquely expressed at a high level in NCI-H358 cells but not in A549 cells.…”