Alkaline phosphatase inhibition based electrochemical sensors for the detection of pesticides

Mazzei, Franco; Botrè, Francesco; Montilla, Simona; Pilloton, Roberto; Podestà, Elisabetta; Botrê, Claudio

doi:10.1016/j.jelechem.2004.08.004

Cited by 85 publications

(34 citation statements)

References 19 publications

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“…In the past we used polyazetidine prepolymer (PAP) to fabricate first generation biosensors (Mascini and Mazzei, 1987;Botrè et al, 1992;Mazzei et al, 1996Mazzei et al, , 2004. In the present work we describe the employment of PAP as immobilizing agent for redox proteins on electrodes with a view to characterizing their electrocatalytic properties in both direct and mediated electron transfer phenomena.…”

Section: Introductionmentioning

confidence: 99%

Polyazetidine-based immobilization of redox proteins for electron-transfer-based biosensors

Frasconi

Favero

Fusco

et al. 2009

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 99%

Polyazetidine-based immobilization of redox proteins for electron-transfer-based biosensors

Frasconi

Favero

Fusco

et al. 2009

Biosensors and Bioelectronics

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“…Also, enzymes based biosensors are often constructed and applied either in amperometric or voltammetric modes [1,2]. Solid surface electrodes, such as carbon paste electrode [3,4], either bare or modified with the adsorption of molecules, have also been designed with different modifications in order to establish electroactivity for pesticides detection [5 -9].…”

Section: Introductionmentioning

confidence: 99%

Study of the Electrochemical Behavior and Sensitive Detection of Pesticides Using Microelectrodes Allied to Square-Wave Voltammetry

Souza

Machado

2006

Electroanalysis

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This work describes the application of gold and carbon fiber microelectrodes allied to square-wave voltammetry for the study of the electrochemical behavior of the organophosphorous insecticides (methyl parathion and dichlorvos) and bipyridilium herbicides (paraquat and diquat), and the development of the sensitive methodology for their analytical determinations in natural water samples. The microelectrodes were lab-made constructed and their electrochemical behavior was characterized by measuring the electrochemical response with a solution of potassium ferricyanide. The experimental and voltammetric conditions to obtain the best analytical signal, in terms of intensities and profile of the peak voltammetric, for four pesticides were optimized and the results were used to evaluate the type of the electrochemical redox process and to appraise the number of electrons covered in each reduction process that occurred for pesticides and also, to propose a possible redox mechanism for a reduction process of pesticides at microelectrodes. Analytical curves were constructed and presented the linear relationships between the peak currents and the concentration of pesticides, for this, the detection limits for pure water (laboratory samples) for four pesticides were calculated and presented values under 15 mg L À1 , lower than maximum limit for drinking water (100 mg L À1 ) permitted by Brazilian Council for groundwater, indicating that the methodology could be employed to analyze those pesticides in natural water samples.

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“…[15][16][17][18][19][20] Como analitos, têm sido investigadas distintas classes de inibidores enzimáticos potenciais como, por exemplo, metais pesados, pesticidas organofosforados e carbamatos, além de sulfitos, peróxidos, tiuréias, e outros. [21][22][23][24] O presente artigo visa uma abordagem no que diz respeito aos processos de inibição, os tipos de biossensores, os procedimentos de confecção mais utilizados (transdutores, mediadores, processos de imobilização), as enzimas mais empregadas e os analitos estudados. Será dada ênfase aos biossensores enzimáticos com transdução eletroquímica, visto que são os mais empregados na literatura.…”

Section: Introductionunclassified

Biossensores baseados no processo de inibição enzimática

Marques¹,

Yamanaka²

2008

Quím. Nova

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Recebido em 3/5/07; aceito em 30/11/07; publicado na web em 4/9/08 BIOSENSORS BASED ON THE ENZYMATIC INHIBITION PROCESS. Fast, selective, reproducible and reliable detections have been carried out by using enzymatic biosensors in several areas. The enzymatic biosensors based on the inhibition represent an important role in analytical chemistry. Enzymes like cholinesterases, peroxidases, tyrosinases, etc. have been immobilized on electrochemical and optical transducers and the enzymatic activity decreasing in the presence of the inhibitor is related with its concentrations. This article presents a review on the enzymes used on the construction of these sensors, emphasizing the respective applications.

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Alkaline phosphatase inhibition based electrochemical sensors for the detection of pesticides

Cited by 85 publications

References 19 publications

Polyazetidine-based immobilization of redox proteins for electron-transfer-based biosensors

Polyazetidine-based immobilization of redox proteins for electron-transfer-based biosensors

Study of the Electrochemical Behavior and Sensitive Detection of Pesticides Using Microelectrodes Allied to Square-Wave Voltammetry

Biossensores baseados no processo de inibição enzimática

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